Beta-N-acetylglucosaminidase in human parotid saliva was separated into two subfractions by diethylaminoethanol cellulose column chromatography. One subfraction of the enzyme was isolated and purified. Disk electrophoresis showed that the purified enzyme was homogeneous. The molecular weight of this enzyme was estimated to be about 153,000 by gel filtration and dodecyl sulfate polyacrylamide gel electrophoresis. N-acetyl-β-galactosaminides also were hydrolyzed by this enzyme at the same site.
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References
1.
Pugh, D.; Leaback, D.H.; and Walker, P.G.: Studies on Glucosaminidase. N-Acetyl-β-glucosaminidase in Rat Kidney, Biochem J65: 464-469, 1957.
2.
Conchie, J.; Findlay, J.; and Levvy, G.A.: Mammalian Glycosidases. Distribution in the Body, Biochem J71: 318-325, 1959.
3.
Findlay, J., and Levvy, G.A.: Purification of β-N-Acetylglucosaminidase from the Pig Epididymis , Biochem J77: 170-175, 1960.
4.
Woollen, J.W. ; Walker, P.G.; and Heyworth , R.: Studies on Glucosaminidase. 6. N-Acetyl-β-glucosaminidase and N-Acetyl-β-galactosaminidase Activities of a Variety of Enzyme Preparations, Biochem J79: 294-298, 1961.
5.
Furiya, S., and Fukuda, A.: Estimation of Serum β-Acetylaminodeoxyglucosidase, J Biochem54: 398-402, 1963.
6.
Weissmann, B. ; Hadjiioannou, S.; and Tornheim, J.: Oligosaccharase Activity of β-N-Acetyl-D-glucosaminidase of Beef Liver, J Biol Chem239: 59-63, 1964.
7.
Caygill, J.C. ; Roston, C.P.J.; and Jevons , F.R.: Purification of β-Acetylglucosaminase and β-Galactosidase from Ram Testis, Biochem J98: 405-409, 1966.
8.
Robinson, D. ; Price, R.G.; and Dance, N.: Separation and Properties of β-Galactosidase, β-Glucosidase, β-Glucuronidase and N-Acetyl-β-glucosaminidase from Rat Kidney, Biochem J102: 525-532, 1967.
9.
Robinson, D. ; Price, R.G.; and Dance, N.: Rat-Urine Glycosidases and Kidney Damage , Biochem J102: 533-538, 1967.
10.
Muramatsu, T.: N-Acetylhexosaminidases from a Gastropod, Turbo cornutus, J Biochem64: 521-531, 1968.
11.
Robinson, D. , and Stirling, J.L.: N-Acetyl-β-glucosaminidases in Human Spleen, Biochem J107: 321-327, 1968.
12.
Koizumi, T.; Suematsu, T.; Iwabori, N.; and Abe, H.: Changes in N-Acetyl-β-glucosaminidase Activity in Hepatic Damage , Biochim Biophys Acta151:628-636, 1968.
13.
Gibson, W.A. , and Fullmer, H.M.: Histochemistry of the Periodontal Ligament. IV. The Glycosidases, J Periodontol40: 470-475, 1969.
14.
Winchester, B.G. : Comparison of the N-Acetylhexosaminidase Components in the Ram Testis and Epididymis by Isoelectric Focusing, Biochem J124:929-934, 1971.
15.
Yoshikawa, J. ; Koide, H.; and Yamanaka, M.: Demonstration of the Multiplicity of N-Acetyl-beta-glucosaminidase by Electrophoresis, Biochem Biophys Res Comm46: 11-15, 1972.
16.
Mega, T.; Ikenaka, T.; and Matsushima, Y.: Studies on N-Acetyl-β-D-glucosaminidase of Aspergillus oryzae. I. Purification and Characterization of N-Acetyl-β-D-glucosaminidase Obtained from Takadiastase , J Biochem68: 109-117, 1970.
17.
Li, S.C. , and Li, Y.T.: Studies on the Glycosidases of Jack Bean Meal. III. Crystallization and Properties of β-N-Acetyl-hexosaminidase , J Biol Chem245: 5153-5160, 1970 .
18.
Menguy, R.; Masters, Y.F.; and Desbaillets, L.: Human Salivary Glycosidases, Proc Soc Exp Biol Med134: 1020-1025, 1970.
19.
Keene, H.J. : Parotid Fluid Collection System with Vacuum-Trap Modification, J Dent Res42: 1041, 1963.
20.
Levvy, G.A., and Conchie, J.: Mammalian Glycosidases and Their Inhibition by Aldonolactons, in Neufeld, E.F., and Ginsburg, V. (eds) : Methods in Enzymology, Vol VIII, New York: Academic Press, 1968, p 580.
21.
Lowry, O.H. ; Rosebrough, N.J.; Farr , A.L.; and Randall, R.J.: Protein Measurement with the Folin Phenol Reagent, J Biol Chem193:265-275, 1951.
22.
Williams, D.E. , and Reisfeld, R.A.: Disc Electrophoresis in Polyacrylamide Gels: Extension to New Conditions of pH and Buffer, Ann NY Acad Sci121: 373-381, 1964.
23.
Andrews, P. : The Gel-Filtration Behaviour of Proteins Related to Their Molecular Weights over a Wide Range, Biochem J96: 595-606, 1965.
24.
Weber, K., and Osborn, M.: The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis, J Biol Chem244: 4406-4412, 1969.
25.
Tiselius, A. ; Hjerten, S.; and Levin, O.: Protein Chromatography on Calcium Phosphate Columns, Arch Biochem Biophys65: 132-155, 1956.
26.
Dixon, M., and Webb, E.C.: Enzymes Catalysing Two Reactions Simultaneously, in Enzymes , 2nd ed, London: Longmans, Green and Co., 1967, p 84.
27.
Dixon, M.: The Determination of Enzyme Inhibitor Constants, Biochem J55: 170-171, 1953.
28.
Borooah, J. ; Leaback, D.H.; and Walker, P.G.: Studies on Glucosaminidase. 2. Substrates for N-Acetyl-β-glucosaminidase, Biochem J78: 106-110, 1961.
29.
Woollen, J.W. ; Heyworth, R.; and Walker, P.G.: Studies on Glucosaminidase. 3. Testicular N-Acetyl-β-glucosaminidase and N-Acetyl-β-galactosaminidase , Biochem78: 111-116, 1961.
30.
Bahl, O.P., and Agrawal, K.M.L.: Glycosidases of Phaseolus vulgaris. I. Isolation and Characterization of β-N-Acetylglucosaminidase , J Biol Chem243: 98-102, 1968.
31.
Bahl, O.P., and Agrawal, K.M.L.: Glycosidases of Aspergillus niger. I. Purification and Characterization of a- and β-Galactosidase and β-N-Acetylglucosaminidase, J Biol Chem244: 2970-2978, 1969.
32.
Frohwein, Y.Z. , and Gatt, S.: Separation of β-N-Acetylglucosaminidase and β-N-Acetylgalactosaminidase from Calf Brain Cytoplasm, Biochim Biophys Acta128: 216-218, 1966.
