Dextranase was prepared from Spicaria violaceae. The in vitro effect of the dextranase on the various polysaccharides produced by oral streptococci was studied. In vivo studies revealed that the dextranase was capable of preventing dental plague deposition in man.
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References
1.
Newbrun, E.: Sucrose, the Arch Criminal of Dental Caries, Odontol Revy18: 373-386, 1967.
2.
Fitzgerald, R.J., and Jordan, H.V.: Polysaccharide-Producing Bacteria and Caries, Harris, R.S. (ed): Art and Science of Dental Caries Research , New York: Academic Press, 1968, pp 79-86.
3.
Guggenheim, B.: Extracellular Polysaccharides and Microbial Plaque, Int Dent J20: 657-678, 1970.
4.
Critchley, P. : The Microbiology of Dental Plaque with Special Reference to Polysaccharide Formation, Dtsch Zahnaerztl Z26: 1155-1161, 1971.
5.
Jeanes, A.; Wilham, C.A.; Jones, R.W.; Tsuchiya, H.M.; and Rist, C.E.: Isomaltose and Isomaltotriose from Enzymic Hydrolyzates of Dextran , J Am Chem Soc75: 5911-5915, 1953 .
6.
Bourne, E.J.; Hutson, D.H.; and Weigel, H.: Studies on Dextrans and Dextranase. 2. The action of Mold Dextranase on Modified Isomaltodextrins and the Effect of Anomalous Linkages on Dextran Hydrolysis, Biochem J85: 158-163, 1962.
7.
Guggenheim, B.: Enzymatic Hydrolysis and Structure of Water-Insoluble Glucan Produced by Glucosyltransferases from a Strain of Streptococcus mutans , Helv Odontol Acta14: 89-108, 1970.
8.
Tsuchiya, H.M. ; Jeanes, A.; Bricker , H.M.; and Wilham, C.A.: Dextran-Degrading Enzymes from Molds, J Bacteriol64:513-519, 1952.
9.
Gibbons, R.J. , and Banghart, S.: Synthesis of Extracellular Dextran by Cariogenic Bacteria and Its Presence in Human Dental Plaque, Arch Oral Biol12: 11-24, 1967.
10.
Guggenheim, B., and Schroeder, H.E.: Biochemical and Morphological Aspects of Extracellular Polysaccharides Produced by Cariogenic Streptococci , Helv Odontol Acta11 : 131-152, 1967.
11.
Bowen, W.H.: Effects of Dextranase on Cariogenic and Non-cariogenic Dextrans, Br Dent124: 347-349, 1968.
12.
Fitzgerald, R.J. ; Spinell, D.M.; and Stoudt, T.H.: Enzymatic Removal of Artificial Plaques , Arch Oral Biol14: 125-128, 1968.
13.
König, K.G. , and Guggenheim, B.: In Vivo Effects of Dextranase on Plaque and Caries, Helv Odontol Acta12: 48-55, 1968.
14.
Tanzer, J.M. ; Wood, W.I.; and Krichevsky , M.I. : Linear Growth Kinetics of Plaque-Forming Streptococci in the Presence of Sucrose, J Gen Microbiol58: 125-133, 1969.
15.
Gehring, V.F. : Ueber die Einwirkung von Dextranase auf Extrazellulär gebildete Streptokokken-Polysaccharide, Dtsch Zahnaerztl Z26:483-489, 1971.
16.
Fitzgerald, R.J.; Keyes, P.H.; Stoudt , T.H.; and Spinell, D.M.: The Effects of a Dextranase Preparation on Plaque and Caries in Hamsters, a Preliminary Repart, JADA76: 301-304, 1968.
17.
Guggenheim, B.; König, K.G. ; Muhlemann, H.R.; and Recolati , B.: Effects of Dextranase on Caries in Rats Harbouring an Indigenous Cariogenic Bacterial Flora, Arch Oral Biol14: 555-558, 1969.
18.
Block, P.L. ; Dooley, C.L.; and Howe, E.E.: The Retardation of Spontaneous Periodontal Disease and the Prevention of Caries in Hamsters with Dextranase, J Periodontol40: 105-110, 1969.
19.
Bowen, W.H. : The Effect of Dextranase on Caries Activity in Monkeys (Macaca irus) , Br Deny J131: 445-449, 1971.
20.
Caldwell, R.C. ; Sandham, H.J.; Mann , W.V.; Finn, S.B.; and Formicola , A.J.: The Effect of a Dextranase Mouthwash on Dental Plaque in Young Adults and Children, JADA82: 124-131, 1971.
21.
Lobene, R.R. : A Clinical Study of the Effect of Dextranase on Human Dental Plaque, JADA82: 132-135, 1971.
22.
Keyes, P.H. ; Hicks, M.A.; Goldman , B.M.; McCabe , R.M.; and Fitzgerald , R.J.: Dispersion of Dextranous Bacterial Plaques on Human Teeth with Dextranase, JADA82: 136-141, 1971.
23.
Zinner D.D. ; Jablon, J.M. ; Aran, A.P.; and Saslaw, M.S.: Experimental Caries Induced in Animals by Streptococci of Human Origin, Proc Soc Exp Biol Med118:766-770, 1965.
24.
Krasse, B.: Human Streptococci and Experimental Caries in Hamsters, Arch Oral Biol11: 429-436, 1966.
25.
Fitzgerald, R.J., and Keyes, P.H.: Demonstration of the Etiologic Role of Streptococci in Experimental Caries in the Hamster, JADA61 : 9-13, 1960.
26.
McCabe, R.M.; Keyes, P.H.; and Howell, A.: An In Vitro Method for Assessing the Plaque Forming Ability of Oral Bacteria , Arch Oral Biol12: 1653-1656, 1967 .
27.
Fitzgerald, R.J. ; Jordan, H.V.; and Stan-Ley , H.R.: Experimental Caries and Gingival Pathologic Changes in the Gnotobiotic Rat, J Dent Res39: 923-935, 1960.
28.
Gibbons, R.J. ; Berman, K.S.; Knoettner , P.; and Kapsimalis , B.: Dental Caries and Alveolar Bone Loss in Gnotobiotic Rats Infected with Capsule Forming Streptococci of Human Origin, Arch Oral Biol11: 549-560, 1966.
29.
Jablon, J.M. , and Zinner, D.D.: Differentiation of Cariogenic Streptococci by Fluorescent Antibody, J Bacteriol92: 1590-1596, 1966.
30.
Zinner, D.Z., and Jablon, J.M.; Human Streptococcal Strains in Experimental Caries, Harris, R.S. (ed) : Art and Science of Dental Caries Research , New York: Academic Press, 1968, pp 87-109.
31.
Wood, J.M., and Critchley, P.: The Extracellular Polysaccharide Produced from Sucrose by a Cariogenic Streptococcus , Arch Oral Biol11: 1039-1042, 1966 .
32.
Hagihara, B. : Crystalline Bacterial Amylase and Proteinase , Ann Rep Sci Works2: 35-80, 1954.
33.
Sugiura, M.; Asano, H.; and Ito, M.: Studies on Dextranase from a Strain of Penicillium, Abstracts of the 90th Annual Congress of the Pharmaceutical Society of Japan 111-143, 1970.
34.
Park, J.T., and Johnson, M.J.: A Submicrodetermination of Glucose, J Biol Chem181: 149-151, 1949.
35.
Ashwell, G.: Methods in Enzymology, Vol III, New York: Academic Press, 1957, pp 73-105.
36.
Dische, Z., and Shettles, L.B.: A Specific Color Reaction of Methylpentoses and Spectrophotometric Micro-Method for Their Determination, J Biol Chem176: 595-603, 1948.
37.
Ghuysen, J.-M.; Tipper, D.J.; and Strom-Inger, J.L. : Enzymes that Degrade Bacterial Cell Walls , in Neufeld, E.F., and Ginsburg, V. (ed) : Methods in Enzymology, Vol VIII, New York: Academic Press , 1966, pp 685-699.
38.
Barry, G.T.; Abbott, V.; and Tsai, T.: Relationship of Colominic Acid (Poly N-Acetylneuraminic Acid) to Bacteria Which Contain Neuraminic Acid, J Gen Microbiol29: 335-352, 1962.
39.
Lowry, O.H. ; Rosebrouch, N.J.; Farr , A.L.; and Randall, R.J.: Protein Measurement with the Folin Phenol Reagent, J Biol Chem193: 265-275, 1951.
40.
Yanagisawa, F.: New Colorimetric Determination of Calcium and Magnesium, J Biochem42: 3-11, 1955.
41.
Lowry, O.H. ; Roberts, N.R.; Leiner , K.Y.; Wu M.L.; and Farr, L.: The Quantitative Histochemistry of Brain. I. Chemical Methods, J Biol Chem207: 1-16, 1954.
42.
Naka, Y.: Purification and Some Properties of Dextranase Obtained from a Strain of Spicaria Species, unpublished manuscript.
43.
Murayama, Y. ; Mihara, T.; Hayashi , H.; Wada, H.; Ebisu , S.; and Yokomizo, I.: Effects of Dextranase from a Strain of Spicaria Species on the Polysaccharides Prepared from Human Dental Plaque, J Jap Org Res Periodont15: 36-41, 1973.
44.
Paunio, I.K. , and Makinen, K.K.: The Phosphate-Liberating Activity of a Mold Neutral Dextranase Preparation, Acta Odontol Scand28:499-504, 1970.
