The teratogenic action of cholesterol in developing rat fetuses was evaluated by the use of 14C-4, 3H-7, and 14C-26 labeled cholesterol. A comparison of radioactivity present in the fetuses indicated that the teratogenic activity of this steroid resides in a metabolite of cholesterol rather than in the intact molecule.
Get full access to this article
View all access options for this article.
References
1.
Buresh, J.J., and Urban, T.J.: The Teratogenic Effect of the Steroid Nucleus in the Rat, J Dent Res43:548-554, 1964.
2.
Buresh, J.J., and Urban, T.J.: Cholesterol Induced Palatal Clefts in the Rat, Arch Oral Biol12:1221-1228, 1967.
3.
Goldwater, W.H., and Stetten, D., JR.: Studies in Fetal Metabolism, J Biol Chem169:723-738, 1947.
4.
Chevallier, F.: Transferets et synthese du cholesterol chez la rat au cours de sa croissance, Biochem Biophys Acta84:316-339, 1964.
5.
Mangold, H.K. : Thin-Layer Chromatography of Lipids, J Am Oil Chem Soc38:708-727, 1961.
6.
Folch, J.; Lees, M.; and Stanley, G.H.S.: A Simple Method for the Isolation and Purification of Total Lipides From Animal Tissue, J Biol Chem226:497-509, 1957.
7.
Kitamura, H. : Epithelial Remnants and Pearls in the Secondary Palate in the Human Abortus: A Contribution to the Study of the Mechanism of Cleft Palate Formations, Cleft Palate J3:240-257, 1966.
8.
Menon, K.M.J. ; Dorfman, R.I.; and For-Chielli , R.: Influence of Gonadotropins on the Cholesterol-Side Chain Cleavage Reaction by Rat Testis Mitochondrial Preparations, Biochem Biophy Acta148:486-494, 1967.
