The taste mechanism was investigated in a primate (Macaca mulatta). Based on the hypothesis that intracellular enzymes contribute to the transduction of tastes to electric impulses by taste cells, a histochemical survey of the activity of several enzymes was made on taste buds from regions of the mouth associated with sweet, salt, sour, and bitter tastes. Considerable differences were noted among the modalities, which confirmed the hypothesis. An exclusively bitter enzyme was identified.
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References
1.
Loven, O.C.: Bidrag till Kannendomen om tungans smakpapiller, Med Arch (Stockholm) 3(9):1, 1867.
2.
Adey, W.R.: The Sense of Smell, in Field, J.; Magoun, H.W.; and Hall, V.E. (eds), Handbook of Physiology, vol I, Washington: American Physiological Society, 1959, p 481.
3.
Baradi, A.F. , and Bourne, G.H.: Localization of Gustatory and Olfactory Enzymes in the Rabbit, and the Problem of Taste and Smell, Nature (Lond) 168: 977, 1951.
4.
Nada, O., and Iwayama, T.: Histochemical Observation on the Phosphatase Activities of the Differentiating Taste Bud, Anat Rec165 (1):61, 1969.
5.
Defendi, V., and Pearson, B.: Quantitative Estimation of Succinic Dehydrogenase Activity in a Single Microscopic Tissue Section, J Histochem Cytochem3:61, 1955.
6.
Farber, E.; Sternberg, W.H.; and Pearce, N.A.M.: Histochemical Localization of Choline Oxidase and D-Amino Acid Oxidase with Tetrazolium Salts and Phenozine Methosulfate, J Histochem Cytochem6: 389, 1958.
7.
Altmann, F.P.: The Cellular Chemistry of Certain Cytoplasmic Oxidative Enzymes and Their Relevance to the Metabolism of Cancers, PhD thesis, University of London, 1968.
8.
Biochemists' Handbook, Long, C. (ed), London: Spon, 1961, p 368.
9.
Hess, R.: Beta-Hydroxybutyric Dehydrogenase Activity in Tumors, Am J Pathol37:583, 1960.
10.
Burstone, M.S. : The Relationship Between Fixation and Techniques for the Histochemical Localization of Hydrolytic enzymes, J Histochem Cytochem6:322, 1958.
11.
Dixon, M., and Webb, E.C.: Enzymes, 2nd ed, London: Longmans, 1964, p 233.
12.
Chiquoine, A.D.: The Distribution of Glucose-6-Phosphatase in the Liver and Kidney of the Mouse, J Histochem Cytochem1: 429, 1953.
13.
Wachstein, M., and Meisel, E.: Histochemistry of Substrate-Specific Phosphatases at a Physiological pH, J Histochem Cytochem4:424, 1956.
14.
Novikoff, A. , and Goldfischer, S.: Nucleotide Diphosphatase Activity in the Golgi Apparatus, Proc Natl Acad Sci47: 802, 1961.
15.
Bourne, G.H. : Alkaline Phosphatase in Taste Buds and Nasal Mucosa, Nature (Lond) 161:444, 1948.
16.
Burstone, M.S. : Histochemical Demonstration of Acid Phosphatases with Naphthol AS-Phosphates, J Natl Cancer Inst19: 523, 1958.
17.
Gomori, G.: Microscopic Histochemistry, Principles and Practice, Chicago : University of Chicago Press, 1952 , p 193.
18.
Pevzner, R.A. : Determination of the Succinic Dehydrogenase Activity in Taste Buds of Some Vertebrate Mammals, Dokl Akad Nauk SSSR155:191, 1964.
19.
Baradi, A.F. , and Bourne, G.H.: New Observations on the Alkaline Phosphatase Reaction in the Papilla Foliata, J Biophys Biochem Cytol5:173, 1959.
20.
Von Bekesy, G.: The Duplexity Theory of Taste, Science145:834, 1964.
21.
Farbman, A.I. : Fine Structure of the Taste Bud, J Ultrastruct Res12:328, 1965.
22.
Nemetschek-Gansler, H., and Ferner, H.: On the Ultrastructure of the Taste Buds, Z Zellforsch Mikrosk Anat63:155, 1964.
23.
Deduve, C.: Lysosomes, a New Group of Cytoplasmic Particles, Hayashi, T. (ed): Subcellular Particles, New York: Ronald, 1959.
24.
Danielli, J.F.: Cytochemistry: A Critical Approach, London: Chapman and Hall, 1953, p 81.
25.
Farber, E.: Principles of Oxidative Enzyme Histochemistry, in Histochemistry, Kansas City: University of Kansas Press, 1964, p 11.
26.
Burstone, M.S. : Histochemical Demonstration of Cytochrome Oxidase with New Amine Reagents, J Histochem Cytochem8:63, 1960.
27.
Altmann, F.P. : The Retention and Estimation of "Soluble" Dehydrogenases in Unfixed Tissue Sections, Biochem J96:53, 1965.
28.
Novikoff, A.; Goldfischer, S.; and Essner, E.: The Importance of Fixation in a Cytochemical Method for the Golgi Apparatus , J Histochem Cytochem9:459, 1961.
29.
Underhay, E.; Holt, S.J.; Beaufay, H.; and Deduge, C.: Intracellular Localization of Esterase in Rat Liver, J Biophys Biochem Cytol2:635, 1956.
30.
West, E.S., and Todd, W.R.: Textbook of Biochemistry, New York: Macmillan, 1961, p 853.
31.
Henkin, R.I. ; Graziadei, P.P.; and Brad-Ley , B.F.: NIH Clinical Staff Conference: The Molecular Basis of Taste and Its Disorders, Ann Intern Med71:791, 1969.
32.
Baradi, A.F. , and Bourne, G.H.: Theory of Taste and Odors, Science113:660, 1951.
