A number of methods for in vitro culture of periodontal ligament fibroblasts were investigated. One successful method is described. Such cells can be maintained readily in subculture. Indications of growth of these cells on sections of enzymatically debrided teeth are reported.
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References
1.
Fleming, H.: Transplantation of Tissue, Baltimore: Williams & Wilkins Co., 1959, pp 272, 289.
2.
Grossman, L.I.: Intential Reimplantation of Teeth, JADA72:1111-1117, 1966.
3.
Nordenram, A.: Autotransplantation of Teeth; A Clinical and Experimental Investigation , Acta Odontol Scand21:70, 1963.
4.
Shulman, L.B., and Kalis, P.J.: A Comparison of Autologous and Allogeneic Tooth Transplants in Rhesus Monkeys , J Oral Surg28:168, 1970.
5.
Dausset, J.; Colombani, J.; Legrand, L.; Feingold, N.; and Rapaport, F.T.: Genetic and Biological Aspects of the HL-A System of Human Histocompatibility , Blood35:591, 1970.
6.
Grupe, H.E.; Ten Cate, A.R.; and Zander, H.A.: A Histochemical and Radiobiological Study of In Vitro and In Vivo Human Epithelial Cell Rest Proliferation, Arch Oral Biol12:1321-1329, 1967.
7.
Merchant, D.J.; Kahn, R.H.; and Murphy, W.H.: Handbook of Cell and Organ Culture, Minneapolis : Burgess Publishing Co., 1964, p 240.
8.
Kollar, E.J., and Baird, G.: Retention of Inductive Potential in Long-Term Cultures of Dental Papillae, abstracted, IADR Program and Abstracts of Papers, No. 336, 1971.
9.
Urist, M.R., and Nogami, H.: Morphogenetic Substratum for Differentiation of Cartilage in Tissue Culture , Nature225:1051, 1970.
