A synthetic medium was developed for further study of intracellular calcification by Bacterionema matruchotii. The medium contained metastable calcium phosphate, six other salts, three purines, two pyrimidines, nine vitamins, pimelic and thioctic acids, vitamin-free casein hydrolysate, and glucose. The vehicle was 0.1 M N-tris (hydroxymethyl) methyl-2-aminoethanesulfonic acid buffer at pH 7.4.
Takazoe, I.: Study on the Intracellular Calcification of Oral Aerobic Leptotrichia, Shikwa Gakuho61:394-401, 1961.
3.
Rizzo, A.A.; Scott, D.B.; and Bladen, H.A.: Calcification of Oral Bacteria, Ann NY Acad Sci109:14-22, 1963.
4.
Ennever, J., and Creamer, H.: Micro-biologic Calcification: Bone Mineral and Bacteria, Cale Tiss Res1:87-93, 1967.
5.
Ennever, J.; Vogel, J.J.; and Takazoe, I.: Calcium Binding by a Lipid Extract of Bacterionema matruchotii, Calc Tiss Res2:296-298, 1968.
6.
Takazoe, I.; Vogel, J.J.; and Ennever, J.: Calcium Hydroxyapatite Nucleation by Lipid Extract of Bacterionema matruchotii , J Dent Res49:395-398, 1970.
7.
Takazoe, I.: A Synthetic Medium for Bacterionema matruchotii, Bull Tokyo Dent Coll10:97-105, 1969.
8.
Reynolds, E.W.: The Use of Lead Citrate at High pH as an Electroopaque Stain in Electron Microscopy , J Cell Biol17 :208-212, 1963.
9.
Good, N.E.; Winget, G.D.; Winter, W.; Connelly, T.N.; Izawa, S.; and Singh, R.M.M.: Hydrogen Ion Buffers for Biological Research, Biochemistry5:467-477, 1966.
10.
Trautz, O.R. ; Lapanta-Le Geros , R.; and Le Geros, J.: Effect of Magnesium on Various Calcium Phosphates. II., abstracted, J Dent Res43:751, 1964.
11.
Mcconnell, D.; Frajola, W.J.; and Deamer, D.W.: Relationship Between the Inorganic Chemistry and Biochemistry of Bone Mineralization, Science133:281-282, 1961.
12.
Mcconnell, D.; Frajola, W.J.; and Deamer, D.W.: Carbonic Anhydrase and the Precipitation of Apatite, Science137: 338, 1962.
