Intracellular protein synthesis and migration in the hamster submandibular gland were studied with light microscope radioautography and histochemistry. The acinar and granular duct cells of the gland had similar rates of protein synthesis and exhibited a polarized migration of nascent protein. Histochemical staining for RNA and protein showed inconsistencies with the radioautographic data.
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References
1.
Schramm, M.: Secretion of Enzymes and Other Macromolecules, Ann Rev Biochem36:307-320, 1967.
2.
Caro, L.C., and Palade, G.E.: Protein Synthesis, Storage and Discharge in the Pancreatic Exocrine Cell: An Autoradiographic Study, J Cell Biol20:473-495, 1964.
3.
Gromet-Elhanan, Z., and Winnick, T.: Microsomes as Sites of a-Amylase Synthesis in the Rat Parotid Gland, Biochim Biophys Acta69:85-96, 1963.
4.
Redman, C.M. ; Siekevitz, P.; and Palade, G.E.: Synthesis and Transfer of Amylase in Pigeon Pancreatic Microsomes, J Biol Chem241:1150-1158, 1966.
5.
Jamieson, J.D. , and Palade, G.E.: Intracellular Transport of Secretory Proteins in the Pancreatic Exocrine CellI. Role of the Peripheral Elements of the Golgi Complex, J Cell Biol34:577-596, 1967.
6.
Jamieson, J.D. , and Palade, G.E.: Intracellular Transport of Secretory Proteins in the Pancreatic Exocrine Cell: II. Transport to Condensing Vacuoles and Zymogen Granules, J Cell Biol34 :597-615, 1967.
7.
Boerner-Patzelt, D.: Die Mundspeicheldrüsen des Goldhamsters, Anat Anz102: 317-332, 1955/1956.
8.
Devi, N.S., and Jacoby, F.: The Submaxillary Gland of the Golden Hamster and Its Postnatal Development , J Anat100: 269-285, 1966.
9.
Jacoby, F., and Leeson, C.R.: The Postnatal Development of the Rat Submaxillary Gland, J Anat93:201-216, 1959.
10.
Messier, B. , and Leblond, C.P.: Preparation of Coated Radioautograms by Dipping Sections in Fluid Emulsion, Proc Soc Exp Biol Med96:7-10, 1957.
11.
Guilford, J.P. : Fundamental Statistics, New York: McGraw Hill Book Co., 1956.
12.
Brachet, J. : The Use of Basic Dyes and Ribonuclease for the Cytochemical Detection of Ribonucleic Acid, J Microsc Sci94:1-10, 1940.
13.
Pearse, A.G.E. : Histochemistry: Theoretical and Applied, 2nd ed, London: J. & A. Churchill Ltd., 1960.
14.
Mazia. D.; Brewer, P.A.; and Alfert, M.: The Cvtochemical Staining and Measurement of Protein with Mercuric Bromphenol Blue, Biol Bull104:57-67, 1953.
15.
Bonhag, P.F. : Histochemical Studies of the Ovarian Nurse Tissues and Oocytes of the Milkweed Bug, J Morph96:381-439, 1955.
16.
Shackleford, J.M., and Schneyer , C.A.: Structural and Functional Aspects of Rodent Salivary Glands Including Two Desert Species, Amer J Anat115:279-308, 1964.
17.
Droz, B., and Warshawsky, H.: Reliability of the Radioautographic Technique for the Detection of Newly Synthesized Protein, J Histochem Cytochem11 :426-435, 1963.
18.
Leblond, C.P. : What Radioautography Has Added to Protein Lore , in LeBlond, C. P., and Warren, K. B. (eds): The Use of Radioautography in Investigating Protein Synthesis, New York: Academic Press, 1965, p 321.
19.
Warshawsky, H. ; Leblond, C.P.; and Droz, B.: Synthesis and Migration of Proteins in the Cells of the Exocrine Pancreas as Revealed by Specific Activity Determination from Radioautographs, J Cell Biol16:1-23, 1963.
20.
Revel, J.P., and Hay, E.D.: An Autoradiographic and Electron Microscope Study of Collagen Synthesis in Differentiating Cartilage, Z Zellforsch61:110-144, 1963.
21.
Ross, R., and Benditt, E.P.: Wound Healing and Collagen Formation, J Cell Biol27:83-106, 1965.
22.
Nadler, N.J. ; Young, B.A.; and Le Blond , C.P.: Elaboration of Thyroglobulin in the Thyroid Follicle, Endocrinol74:333-354, 1964.
23.
Reith, E.J. : Collagen Formation in Developing Molar Teeth of Rats, J Ultrastruct Res21:383-414, 1968.
24.
Shackleford, J.M., and Klapper, C.E.: Structure and Carbohydrate Histochemistry of Mammalian Salivary Glands, Amer J Anat111:25-48, 1962.
25.
Flon, H., and Gerstner, R.: Salivary Glands of the Hamster: I. The Submandibular Gland: A Histochemical Study After Preservation with Various Fixatives, Acta Histochem31:234-253, 1968.
26.
Kunkel, H.G. , and Tiselius, A.: Electrophoresis of Proteins on Filter Paper , J Gen Physiol35:89-118, 1951.
27.
Rasch, E., and Swift, H.: Microphotometric Analysis of the Cytochemical Mil-Ion Reaction, J Histochem Cytochem8: 4-17, 1960.
28.
Kent, P.W.: Structure and Function of Glycoproteins, in Campbell, P. N., and Grevelle, G. D. (eds): Essays in Biochemistry, New York: Academic Press, 1967, pp 105-151.
29.
Shackleford, J.M.: Histochemical Comparison of Mucous Secretions in Rodent, Carnivore, Ungulate, and Primate Major Salivary Glands, Ann N Y Acad Sci106: 572-582, 1963.
30.
Sreebny, L.M., and Meyer, J.: Hormones, Inanition and Salivary Glands, in Sreebny, L. M., and Meyer, J. (eds): Salivary Glands and Their Secretions, New York: Macmillan Co., 1964, pp 83-102.
31.
Ficq, A. , and Brachet, J.: Distribution de l'Acide Ribonucleique et Incorporation de Phénylalanine-214C dans les Proteins, Exp Cell Res11:135-145, 1956.
32.
Allfrey, V.A. ; Daly, M.M.; and Mirsky, A.E.: Synthesis of Protein in the Pancreas, J Gen Physiol37:157-175, 1953/ 1954.
33.
Pease, D.C. : Infolded Basal Plasma Membranes Found in Epithelia Noted for Their Water Transport, J Biophys Biochem Cytol, 4(suppl):203-208, 1956.
