Calcium ions enhanced the adsorption of salivary proteins by hydroxyapatite. Calcium probably acted in the protein-bound form by promoting the aggregation of proteins. Concentrations of calcium far below physiologic levels exerted maximal efJects. Lowering the pH to 5 increased protein adsorption but abolished the effect of calcium. Relevance to the in vivo situation was examined.
Get full access to this article
View all access options for this article.
References
1.
Meckel, A.H. : The Formation and Properties of Organic Films on Teeth, Arch Oral Biol10:585-597, 1965.
2.
Armstrong, W.G. : The Composition of Organic Films Formed on Human Teeth, Caries Res1:89-103, 1967.
3.
McGaughey, C. , and Stowell, E.C.: The Adsorption of Human Salivary Proteins and Porcine Submaxillary Mucin by Hydroxyapatite, Arch Oral Biol12:815-828, 1967.
4.
Itzhaki, R.F. : A Micro-Biuret Method for Estimating Protein , Anal Biochem9 :401-410, 1964.
5.
Harnach, F.A. : The Analysis of Calcium in Unashed Saliva , J Dent Res39 :1069-1071, 1960 .
6.
Ericson, T.: Adsorption to Hydroxylapatite of Proteins and Conjugated Proteins from Human Saliva, Caries Res1:52-58, 1967.
7.
Ericson, T.: Salivary Glycoproteins. Composition and Adsorption to Hydroxylapatite in Relation to the Formation of Dental Pellicles and Calculus, Acta Odont Scand26:3-21, 1968.
8.
Mcgaughey, C., and Stowell, E.C.: Effects of H+ and Cl- on Adsorption of Salivary Proteins by Hydroxyapatite, abstracted, IADR Program and Abstracts of Papers, No. 91, 1970 .
9.
Jenkins, G.N. : The Influence of Environmental Fluids on Enamel Solubility, J Dent Res45 (suppl) :662-671, 1966.
10.
Hay, D.I. : Some observations on Human Saliva Proteins and Their Role in the Formation of Acquired Enamel Pellicle, J Dent Res48 (suppl):806-810, 1969.
11.
Lightfoot, L.; Besic, F.C.; Harnach , F.; and Coolidge, T.B.: Ultrafilterable Calcium in Caries Susceptible and Caries Immune Subjects, J Dent Res40:311-313, 1961.
12.
Brudevold, F.; Groen, P., and McCann , H.: Physio-chemical Aspects of the Enamel-Saliva System, Advances Fluorine Res3:63-78, 1965.
13.
McGaughey, C. , and Stowell, E.C.: The Influence of Calcium and Hydrogen Ions on the Precipitation and Adsorption by Hydroxyapatite of Salivary Proteins, abstracted, IADR Program and Abstracts of Papers, No. 168, 1969.
