Purified acid phosphomonoesterase from human parotid saliva has been characterized as an acid nucleotidase of broad specificity. The enzyme exhibited a pH optimum at 5.8 with 5'-AMP and was not metal dependent. Activity was inhibited greatly by mercuric ions, sodium fluoride, and sodium tartrate. The tartrate inhibition was competitive.
Get full access to this article
View all access options for this article.
References
1.
Nakamura, R. ; Watanabe, T.; Iwamoto, Y.; and Tsunemitsu , A.: Purification and Properties of Acid Phosphomonoesterase from Human Parotid Saliva, J Dent Res49:561-566, 1970.
2.
Brightwell, R., and Tappel, A.L.: Lysosomal Acid Pyrophosphatase and Acid Phosphatase, Arch Biochem Biophys124: 333-343, 1968.
3.
Hafkenscheid, J.C.M.: Properties of an Acid Phosphatase in Escherichia coli, Biochim Biophys Acta167:582-589, 1968.
4.
Arsenis, C., and Touster,O.: Purification and Properties of an Acid Nucleotidase from Rat Liver Lysosomes , J Biol Chem243:5702-5708, 1968 .
5.
Igarashi, M. , and Hollander, V.P.: Acid Phosphatase from Rat Liver, J Biol Chem243:6084-6089, 1968.
6.
Allen, R.J.L. : The Determination of Phosphorus, Biochem J34:858-865, 1940.
7.
Bessey, O.A. ; Lowry, O.H.; and Brock, M.J.: A Method for the Rapid Determination of Alkaline Phosphatase with 5 mm3 of Serum, J Biol Chem164:321-329, 1946.
8.
Lowry, O.H.; Rosebrough, N.J.; Farr, A.L.; and Randall, R.J.: Protein Measurement with the Folin Phenol Reagent, J Biol Chem193:265-275, 1951.
9.
Lisowski, J. : 5'-Ribonucleotide Phosphohydrolase from Bovine Pituitary Gland, Biochim Biophys Acta113:321-331, 1966.
10.
Song, C.S., and Bodansky,O. : Purification of 5'-Nucleotidase from Human Liver, Biochem J101:5c, 1966.
11.
Center, M.S. , and Behal, F.J.: Calf Intestinal 5'-Nucleotidase, Arch Biochem Biophys114:414-421, 1966.
12.
Song, C.S., and Bodansky,O. : Subcellular Localization and Properties of 5'-Nucleotidase in the Rat Liver, J Biol Chem242: 694-699, 1967.
13.
Itoh, R.; Mitsui, A.; and Tsushima, K.: 5'-Nucleotidase of Chicken Liver, Biochim Biophys Acta146:151-159, 1967.
14.
Fritzson, P. : Nucleotidase Activities in the Soluble Fraction of Rat Liver Homogenate, Biochim Biophys Acta178:534-541, 1969.
15.
Brown, E.B. , JR., and Stadtman , E.R.: Nucleotidase of Clostridium propionicum, J Biol Chem235:2928-2934, 1960.
16.
Neu, H.C. : The 5'-Nucleotidase of Escherichia coli, J Biol Chem242:3896-3911, 1967.
17.
Björk, W.: Activation and Stabilization of Snake-Venom 5'-Nucleotidase, Biochim Biophys Acta89:483-494, 1964.
18.
Shuster, L. , and Kaplan, N.O.: A Specific b Nucleotidase, J Biol Chem201:535-546, 1953.
19.
Lionetti, F.J. ; Chauncey, H.H.; and Li-Santi , V.F.: Human Parotid Salivary Acid Phosphomonoesterase, Biochim Biophys Acta24:496-502, 1957.
