An organ culture method is described in which bone matrix was successfully formed.
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References
1.
Pritchard, J.J. : A Cytological and Histochemical Study of Bone and Cartilage Formation in the Rat, J Anat86:259-277, 1952.
2.
McLean, F.C., and Bloom, W.: Calcification and Ossification. Calcification in Normal Growing Bone, Anat Rec78 :333-359, 1940.
3.
Heller-Steinberg, M.: Ground Substance, Bone Salts, and Cellular Activity in Bone Formation and Destruction, Amer J Anat89:347-379, 1951.
4.
LoE, H.: Bone Tissue Formation. A Morphologic and Histochemical Study, Acta Odont Scand17 (suppl):311-427, 1959.
5.
Fell, H.B.; Mellanby, E.; and Pelc, S.R.: Influence of Excess Vitamin A on the Sulfate Metabolism on Bone Rudiments Grown In Vitro, J Physiol134:179-188, 1956.
6.
Heyner, S., and Biggers, J.D. : Cultivation of Isolated Embryonic Rat Tibiae on a Chemically Defined Medium, Nature182: 810-811, 1958.
7.
Sevastikoglou, J.A.: Osteogenesis in Tissue Culture. A Morphologic and Biochemical Study, Acta Orthop Scand, suppl 33, 1958.
8.
Biggers, J.D. ; Gwatkin, R.B.L.; and Heyner , S.: Growth of Embryonic Avian and Mammalian Tibiae on a Relatively Simple Chemically Defined Medium , Exp Cell Res25:41-58, 1961.
9.
Shaw, J.L., and Bassett, C.A.L.: An Improved Method for Evaluating Osteogenesis In Vitro, Anat Rec149:57-66, 1964.
10.
Prasad, G.C. , and Reynolds, J.J.: Effect of Environmental Factors on the Repair of Bone In Vitro, J Bone Joint Surg50B: 401-408, 1968.
11.
Porter, K.: In Vitro Culture of Mucosa, J Dent Res39:67-75, 1960.
12.
Porter, K.: Production of Bone Matrix In Vitro: II. Histochemical Observations on the Role of Acid Mucopolysaccharide in Early Matrix Formation, J Dent Res50:41-47, 1971.
13.
Shaw, J.L., and Bassett, C.A.L.: The Effects of Varying Oxygen Concentrations on Osteogenesis and Embryonic Cartilage In Vitro, J Bone Joint Surg49A:73-80, 1967.
14.
Hairstone, M.A. : Structural Cytology of the Healing WoundI. The Fibroblast, Oral Surg12:1484-1495, 1959 .
15.
McLean, F.C., and Urist, M.R.: Bone: Fundamentals of the Physiology of Skeletal Tissue, 3rd ed, Chicago: University of Chicago Press, 1968, p 5.
16.
McLean, F.C., and Urist, M.R.: Bone: Fundamentals of the Physiology of Skeletal Tissue, 3rd ed, Chicago: University of Chicago Press, 1968, p 5-6.
17.
Leriche, R., and Policard, A.: The Normal and Pathological Physiology of Bone, Its Problems, St. Louis, Mo: C. V. Mosby Co., 1928, p 50 (translated from the French by S. Moore and J. A. Key).
18.
Leriche, R., and Policard, A.: The Normal and Pathological Physiology of Bone, Its Problems, St. Louis, Mo: C. V. Mosby Co., 1928, p 43 (translated from the French by S. Moore and J. A. Key).