Cultures of oral mucosa from 15- to 17-day-old rat fetuses were placed under cellophane membranes on cover glasses of Rose chambers to determine the action of low doses of X radiation on the oral epithelial cells, exclusive of mitotic phenomena. The principal characteristic of the emigrating cells in control cultures was established. The most important changes in surviving cells of the irradiated cultures were the formation of multinucleated giant cells, cellular vacuolization, and movements of the cell membranes (zeiosis and pericyclosis). Even with the low doses, there was a decided inhibition of growth.
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References
1.
Loeb, L.: Über die Entstehung von Bindegewebe, Leucocyten und roten Blutkörperchen aus Epithel und über cine Methode, isolierte Gewebsteile zu züchten, Chicago: M. Stem and Co., 1897.
2.
Harrison, R.G. : Observations on the Living Developing Nerve Fibers. Proc Soc Exp Biol Med4:140-43, 1906-1907.
3.
Glasstone, S. : The Development of Tooth Germs in Vitro, J Anat70:260-66, 1936. 4. Lefkowitz, W.; Mardfin, D.F.; and Bo-Decker, C.F. : Cultivation of Rat Molar Tooth Germs in Carrel Flasks, J Dent Res33:189-200, 1954.
4.
Nizima, M.: Enamel Epithelium in Tissue Culture, Am J Anat99:351-389, 1956.
5.
Nizima, M.: Tissue Culture of an Ameloblastoma, Z Zellforsch46:127-138, 1957. 7. Nizima, M., and Cattoni, M.Dental Papila in Tissue Culture, J Dent Res37 :767-779, 1958.
6.
Shafer, W.G. : Effect of Dilantin Sodium on Growth of Human Fibroblast-like Cell Cultures, Proc Soc Exp Biol Med104 :198-201, 1960.
7.
Shafer, W.G. : Cell Stimulatory Effect on Diphenylhydantoin in Tissue Culture, abstracted, J Dent Res40:680, 1961.
8.
Silverman, S., JR., and Donoghue , J.: A Continuous Culture (WiSh) Derived from a Human Gingival Carcinoma, J Dent Res39:572-577, 1960.
9.
Rose, G.G.; Cattoni, M.; and Pomerat, C.M.: Tissue Culture Study of Human Gingiva : I. The Morphological Diversity of Cells When Using the Cellophane Membrane Techniques, J Dent Res41:997-1012, 1962.
10.
Rose, G.G.; Cattoni, M.; and Pomerat, C.M.: Observations on Oral Tumors in Vitro: I. Zeiosis Versus the Nucleus and Endoplasmic Reticulum in Strain KB, J Dent Res42:38-53, 1963.
11.
Rose, G.G.; Cattoni, M.; and Pomerat, C.M.: Observations on Oral Tumors In Vitro: 2. Karyobiosis in Strain KB Cells, J Dent Res43:580-605, 1964.
12.
Rose, G.G.: A Separable and Multipurpose Tissue Culture Chamber, Texas Rep Biol Med12:1074-1083, 1954 .
13.
Rose, G.G.: Special Uses of the Multipurpose Tissue Culture Chamber, Texas Rep Biol Med15:310-312, 1957.
14.
Rose, G.G.; Pomerat, C.M.; Shindler, T.O.; and Trunnell, J.B.: A Cellophane-Strip Technique for Culturing Tissue in Multipurpose Culture Chambers, J Biophys Biochem Cytol4:761-764, 1958.
15.
Nease, W.J. : Effect of Sodium Diphenylhydantoinate on Tissue Cultures of Human Gingiva, J Periodont36:22-33, 1965.
16.
Rose, G.G.: Observations on Oral Tumors In Vitro: III. Morphological Properties, Longevity of Nonviral Intranuclear Inclusions, J Nat Cancer Inst36:747-773, 1966. 19. Smulow, J.B., and Glickman, I.: An Epithelial-like Cell Line in Continuous Cultures from Normal Adult Human Gingiva, abstracted, IADR Program and Abstracts of Papers, No. 256, 1966.
17.
Kumegawa, M. ; Cattoni, M.; and Rose, G.G.: Electron Microscopic Studies of Oral Cells In Vitro: I. Annulate Lamellae Observed in Strain KB Cells, J Cell Biol34: 897-901, 1967.
18.
Kumegawa, M. ; Cattoni, M.; and Rose, G.G.: Electron Microscopic Studies of Oral Cells In Vitro: II. Subsurface and Intracytoplasmic Confronting Cisternae in Strain KB Cells, J Cell Biol36:443-452, 1968. 22. Powell, R.N.: Gingival Tissue Physiology
19.
In Vitro I. Gingival Organ Culture:J Periodont Res2:290-96, 1967.
20.
Quastel, M.R. , and Pomerat, C.M.: Irradiation of Cells in Tissue Culture , Z Zellforsch59:214-23, 1963.
21.
Dickson, M.; Paul, J.; and Davison, J.N.: The Effect of X-Radiation on Cultured Cells, abstracted, Biochem J70:18P, 1958. 25. Hoffman, R.S., and Wollman, S.H.: Effects of X-Rays on the Migration of Cells From Adult Tissue Explants, Proc Soc Exp Biol Med70:38-40, 1949.
22.
Lasnitzki, I. : The Effect of X-Rays on Cells Cultivated In Vitro, Brit J Radiol13 :279-284, 1940.
23.
Reid, T.R., and Gifford, M.P.: A Quantitative Study of the Effects of X-Radiation on Cells In Vitro , J Nat Cancer Inst13: 431-39, 1952.
24.
Teresamina, T., and Tolmache, L.J.: Changes in X-Ray Sensitivity of HeLa Cells During the Division Cycle, Nature190:1210, 1961 .
25.
Alescio, T. : Response to X-Irradiation of Mouse Embryonic Lung Cultured In Vitro, Exp Cell Res43:459-473, 1966.
26.
Trowell, O.A.: Tissue Culture in Radio-
27.
biology , in Willmer, E.N. (ed), Cells and Tissues in Culture: Methods, Biology and Physiology, Vol 3, New York: Academic Press, Inc., 1966, pp 63-149.
28.
, Costero, I. , and Pomerat, C.M.: Cultivation of Neurons from the Adult Human Cerebral and Cereberal Cortex , Amer J Anat89:405-468, 1951.
29.
Sherwin, R.P. ; Richters, A.; and Richters, V.: The Occurrence of a Cyclosislike Phenomenon in Human Lung Cancer Cells In Vitro, Cancer Res27:152-58, 1967. 33. Rose, G.G.: Phase Contrast Microscopy in Living Cells, J Roy Micr Soc83:97-114, 1964.
30.
Rose, G.G., and Cattoni, M.: Mosaic Patterns of Stromal Cells in Tissue Culture, in Rose, G.G. (ed), Cinemicrography in Cell Biology, New York: Academic Press, Inc., 1963, pp 445-469.
31.
Gallagher, W.N. : Dental Roentgenology Review, New York: William Frederick Press, 1967, p 80.
32.
Jepsen, A., and Theilade, J.: Electron Microscope Identification of Epithelial Cells in Tissue Culture of Oral Mucous Membrane, J Cell Biol32:770-772, 1967.
