Incorporation of 3H Thymidine into Damaged Pulp of Rat Incisors

Abstract

³H thymidine was injected intraperitoneally into six male rats on the first, second, and third days after injury to the pulp of the incisors. Forty minutes later the rats were decapitated; the teeth were fixed in formalin and prepared in the usual manner for histoautoradiography. Histologic preparations were stained with hematoxylin and eosin. No odontoblast nuclei labeled by ³H thymidine were observed on the histoautoradiographic pattern. After pulp injury, the odontoblasts behaved as irreversible postmitotic cells incapable of DNA synthesis.

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