The electron microprobe was used to analyze the calcium and phosphorus concentrations of sound, acid-etched, and remineralized enamel. By use of a weak acetic acid-potassium acetate buffer, it was found that the demineralization probably affected only the first 10 micrometers of the enamel surface. This demineralized enamel was successfully remineralized by the use of a calcifying solution. The changes in calcium and phosphorus concentrations and the Ca/P ratios were determined.
Get full access to this article
View all access options for this article.
References
1.
Koulourides, T., and Pigman, W.: Studies on Rehardening of Artificially Softened Enamel, J Dent Res39:198, 1960.
2.
Koulourides, T.; Cueto, H.; and Pigman, W.: Rehardening of the Softened Enamel Surfaces of Teeth by Solutions of Calcium Phosphates , Nature 189:4760, 226-227, 1961.
3.
Koulourides, T.: Remineralization of Enamel and Dentin, Dent Clin of N Amer485-497, 1962.
4.
Koulourides, T.; Feagin, F.; and Pigman, W.: Remineralization of Dental Enamel by Saliva in Vitro, Annals NY Acad Sci131:685-693, 1965.
5.
Pigman, W.; Cueto, H.; and Baugh, D.: Conditions Affecting the Rehardening of Softened Enamel, J Dent Res43 :1187-1195, 1964.
6.
Pickel, F.D.; Cort, W.; Starr, N.; Bilotti, A.; and Pigman, W.: Evaluation of Enamel Rehardening Agents in Saliva, J Dent Res44:855-859, 1965.
7.
Brudevold, F.; Amdur, B.H.; and Mes-Ser, A.: Factors Involved in Remineralization of Carious Lesions, Arch Oral Biol6:304, 1961.
8.
Wei, S.H.Y.: Remineralization of Enamel and Dentine: A Review, J Dent Child34: 444-451, 1967.
9.
Koulourides, T.: "Dynamics of Tooth Surface-Oral Fluid Equilibrium," in Staple, P. H. (ed) Advances in Oral Biology, Vol 2, New York: Academic Press , 1966, pp 149-169.
10.
Applebaum, E.: The Radiopaque Surface Layer of Enamel and Caries, J Dent Res19:41-46, 1940.
11.
Applebaum, E.: Tissue Changes in Caries, Dent Cosmos77:931-941, 1935.
12.
Amprino, R. , and Camanni, F.: Historadiographic and Autoradiographic Researches of Hard Dental Tissues, Acta Anat28:217-258, 1956.
13.
Lenz, H., and Muhlemann, H.R.: In Vivo and In Vitro Effects of Saliva on Etched or Mechanically Marked Enamel after Certain Periods of Time, Helv Odont Acta7:30-33, 1963.
14.
Lenz, H., and Muhlemann, H.R.: Repair of Etched Enamel Exposed to the Oral Environment, Helv Odont Acta7:47-49, 1963.
15.
Muhlemann, H.R.; Lenz, H.; and Ros-Sinsky , R.: Electron Microscopic Appearance of Rehardened Enamel, Helv Odont Acta8:108-111, 1964.
Mcdougall, W.A. , and Adkins, K.F.: A Method for the in Vitro Study of the Demineralization and Remineralization of the Sub-Surface Enamel, Aust Dent J11: 20-26, 1966.
18.
Boyde, A.; Switsur, V.R.; and Fearn-Head, R.W. : Application of the Scanning Electron Probe X-Ray Microanalyzer to Dental Tissues, J Ultrastruct Res5 :201-207, 1961.
19.
Frazier, P.D. : Electron Probe Analysis of Human Teeth: Some Problems in Sample Preparation, Norelco Reporter8 :25-27, 1966.
20.
Rosser, H.; Boyde, A.; and Stewart, A.D.G.: Preliminary Observations on the Calcium Concentration in Developing Enamel Assessed by Scanning Electron-Probe X-Ray Emission Microanalysis, Arch Oral Biol12:431-440, 1967.
21.
Wei, S.H.Y. , and Koulourides , T.: Electron Microprobe Analyses of Sound, Demineralized and Remineralized Enamel, abstracted IADR Program and Abstracts of Papers, No. 539, 1969.
22.
Wei, S.H.Y. , and Ingram, M.J.: Analyses of the Amalgam-Tooth Interface Using the Electron Microprobe, J Dent Res48: 317-320, 1969.
23.
Brudevold, F. , and Soremark, R.: "Chemistry of the Mineral Phase of Enamel ," in Miles, A.E.W. (ed) Structural and Chemical Organization of Teeth, Vol II, New York: Academic Press, 1967, p 249.
24.
Frank, R.M. ; Capitant, M.; and Goni, J.: Electron Probe Studies of Human Enamel, J Dent Res45:672-682, 1966.
25.
Soremark, R. , and Samsahl, K.: Gammary Spectrometric Analysis of Elements in Normal Human Enamel, Arch Oral Biol6:275-283, 1961.
26.
Besic, F.C. ; Knowles, C.R.; Wiemann , M.R., JR.; and Keller , 0.: Electron Probe Microanalysis of Noncarious Enamel and Dentin and Calcified Tissues in Mottled Teeth, J Dent Res48:131-139, 1969.
27.
Frazier, P.D. : Electron Probe Analysis of Human Teeth. Ca/P Ratios in Incipient Carious Lesions, Arch Oral Biol12: 25-33, 1967.