The concentrations of certain peptide hydrolases and enzymes of the aerobic and anaerobic catabolic cycles in the fibroblasts of bovine dental pulp have been examined as a function of the developmental stage of the tissue. It was shown that the enzyme content does not change uniformly during the aging process. Anaerobic catabolic pathways remained unchanged or increased with cell maturity whereas the citric acid cycle activity decreased. Peptide hydrolases remained essentially constant.
Get full access to this article
View all access options for this article.
References
1.
Fisher, A.K. ; Belding, J.H.; Opinsky, J.S.; and Spinella , D.J.: The Influence of the State of Tooth Development on the Oxygen Quotient of Normal Bovine Dental Pulp, J Dent Res38:208, 1959.
2.
Delbrueck, A. : Untersuchungen ueber Enzyme des Energie-Stoffwechsels im Bindegewege, Klin Wschr40:677, 1962.
3.
Nadler, H.L. : Patterns of Enzyme Development Utilizing Cultivated Human Fetal Cells Derived from Amniotic Fluid, Biochem Genet2:119, 1968.
4.
Schwabe, C., and Kalnitsky, G.: A Peptidohydrolase from Mammalian Fibroblasts (Bovine Dental Pulp), Biochemistry (Wash)5:158, 1966.
5.
Schwabe, C.: Peptide Hydrolases in Mammalian Connective Tissue: I. Survey of Activities and Preliminary Characterization of Certain Peptidases. Biochemistry (Wash)8: 771, 1969.
6.
Schwabe, C.: Peptide Hydrolases in Mammalian Connective Tissue. II. Leucine Aminopeptidase : Purification and Evidence for Subunit Structure, Biochemistry (Wash)8: 783, 1969.
7.
Matheson, A.T. , and Tattri, B.L.: A Modified Yemm and Cocking Ninhydrin Reagent for Peptidase Assay, Canad J Biochem42:95, 1964.
8.
Stolzenbach, F.: Methods in Enzymology, Vol. 9, Wood, W.A. (ed.), New York: Academic Press , 1966, p 278.
9.
Ochoa, S.: Methods in Enzymology, Vol. 1, Colowick, S.P. and Kaplan N.O. (eds.), New York: Academic Press, 1955, p 735.
10.
Massey, V.: Methods in Enzymology, Vol. 1, Colowick, S.P. and Kaplan, N.O. (eds.), New York: Academic Press, 1955, p 729.
11.
Goldberg, E.: Annals of The New York Academy of Sciences, Vol. 121, Article2, 1964, p 500.
12.
Marks, P.A.: Methods in Enzymology, Vol. 9, Wood, W.A. (ed.), New York: Academic Press , 1966, p 134.
13.
Clarke, J.T. : Annals of The New York Academy of Sciences , Vol. 121, Article2, 1964, p 428.
14.
Schwabe, C.: Leucine Aminopeptidase. A Calcium Phosphate Gel Complex, Biochemistry (Wash)8:795, 1969.