A deficiency of ascorbic acid caused a decreased uptake of 3H-proline into guinea pig skin collagen and a further decrease in the 3HO-proline formation; inanition from pair-feeding was not a contributing factor. An anemia developed in the completely deficient animals which was prevented with low levels of ascorbic acid in another group.
Get full access to this article
View all access options for this article.
References
1.
Wolbach, S.B. , and Howe, P.R.: Intracellular Substances in Experimental Scorbutus, Arch Pathol1:1-24, 1926.
2.
Hutton, J.J. ; Tappel, A.L.; and Udenfriend , S.: Cofactor and Substrate Requirements of Collagen Proline Hydroxylase, Arch Biochem Biophys118:231-240, 1967.
3.
Manning, J.M. , and Meister, A.: Conversion of Proline to Collagen Hydroxyproline , Biochem5:1154-1165, 1966 .
4.
Robertson, W.Van B.; Hewitt, J.; and Herman, C.: The Relation of Ascorbic Acid to the Conversion of Proline to Hydroxyproline in the Synthesis of Collagen in Carrageenan Granuloma , J Biol Chem234:105-108, 1959.
5.
Robertson, W.Van B.: The Biochemical Role of Ascorbic Acid in Connective Tissue, Ann N Y Acad Sci92:159-167, 1961.
6.
Gould, B.S. : Ascorbic Acid-Independent and Ascorbic Acid-Dependent Collagen Forming Mechanisms, Ann N Y Acad Sci92:168-174, 1961.
7.
Evans, J.R.; Hughes, R.E.; and Thomas, R.Z.: Nitrogen Metabolism in Guinea Pigs Receiving Low Daily Intakes of Ascorbic Acid, Proc Nutr Soc24:11, 1965.
8.
Roe, J.H.: "Chemical Determinations of Ascorbic, Dehydroascorbic and Diketogulonic Acids," in Glick, David (ed.): Methods of Biochemical Analyses I, New York: Interscience Publishers, Inc., 1954, pp 115-139.
9.
Fitch, S.M.; Harkness, M.L.; and Harkness, R.D.: Extraction of Collagen from Tissues, Nature176:163-164, 1955.
10.
Lowry, O.H.; Rosebrough, N.J.; Farr, A.L.; and Randall, R.J.: Protein Measurement with the Folin-Phenol Reagent, J Biol Chem193:265-275, 1951.
11.
Pashley, D.H. ; Claycomb, C.K.; and Summer, G.W.: Liberation of Hydroxyproline from Gelatin by Acid Hydrolysis, Anal Biochem15:154-156, 1966.
12.
Leach, A.A. : Notes on a Modification of the Neuman and Logan Method for the Determination of the Hydroxyproline, Biochem J74:70-71, 1960.
13.
Chinard, F.P. : Photometric Estimation of Proline and Ornithine , J Biol Chem199:91-95, 1952.
14.
Harfenist, E.J.: The Amino Acid Compositions of Insulins Isolated from Beef, Pork, and Sheep Glands, J Am Chem Soc75:5528-5533, 1953.
15.
Gesehwind, I.I.; Williams, B.S.: and Li, C.H.: Simplification of the Roe and Keuther Procedure for Ascorbic Acid Determination in Adrenal Glands, Acta Endocrinol8:247-250, 1951.
16.
Reid, M.E.: "The Guinea Pig in Research," in Biology, Nutrition, Physiology, Washington, DC: Publication of the Human Factors Research Bureau, Inc., No. 557, 1965, pp 40-44.
17.
Crampton, E.W. ; Collier, B.C.; Woolsey, L.D.; and Farmer, F.A.: A New Procedure for Bioassay of Vitamin C, Science100: 599-601, 1944.
18.
Dunker, C.F. ; Fellers, C.R.; and Esseln, W.B., Jr.: A Comparison of Four Methods for Determining Vitamin C with a 25-Day Weight Response Bioassay, Food Research7:260, 1942.
19.
Hughes , R.E.: Reduction of Dehydroascorbic Acid by Animal Tissues, Nature203:1068-1070, 1964 .
20.
Schneider, W., and Staudinger , J.H.: Reduced Nicotinamide-adenine Dinucleotide-Dependent Reduction of Semidehydroascorbic Acid, Biochim Biophys Acta96 :157-159, 1965.
21.
Clayton, B.E. ; Mcswinney, R.R.; and Prunty, F.I.G.: Metabolism of Dehydroascorbic Acid and the Effect of Adrenocorticotrophic Hormone and Cortisone, Biochem J58:542-552, 1954.
22.
Crampton, E.W. : Growth of Odontoblasts of the Incisor Tooth as a Criterion of the Vitamin C Intake of the Guinea Pig, J Nutr33:491-504, 1947.
23.
Keuther, C.A. ; Telford, I.R.; and Roe, J.H.: The Relation of the Blood Level of Ascorbic Acid to the Tissue Concentration of this Vitamin and to the Histology of the Incisor Teeth in the Guinea Pig, J Nutr28: 347-358, 1944.
24.
Key, K.M. , and Morgan, B.G.E.: The Determination of the Vitamin C Value of Ascorbic Acid, Biochem J27:1030-1035, 1933.
25.
Bourne, G.H. : The Effect of Vitamin C on the Healing of Wounds , Proc Nutr Soc4: 204-210, 1946.
26.
Frederici, H.H.R.; Taylor, H.; Rose, R.; and Pirani, C.L.: The Fine Structure of Capillaries in Experimental Scurvy, Lab Invest15:1442-1458, 1966 .
27.
Robertson, W. Van B., and Hewitt , J.: Augmentation of Collagen Synthesis by Ascorbic Acid In Vitro, Biochim Biophys Acta49:404-406, 1961.
28.
Hutton, J.J.; Tappel, A.L.; and Udenfriend , S.: Requirements for a-Ketoglutarate, Ferrous Ion and Ascorbate by Collagen Proline Hydroxylase, Biochem Biophys Res Commun24:179-184, 1966.
29.
Peterkofsky, B., and Udenfriend , S.: Enzymatic Hydroxylation of Proline in Microsomal Polypeptide Leading to Formation of Collagen, Proc Natl Acad Sci53: 335-342, 1965.
30.
Gould, B.S. , and Woessner , J.F.: Biosynthesis of Collagen. The Influence of Ascorbic Acid on the Proline, Hydroxyproline, Glycine, and Collagen Content of Regenerating Guinea Pig Skin, J Biol Chem226: 289-300, 1957.
31.
Robertson, W.Van B., and Schwartz, B.: Ascorbic Acid and the Formation of Collagen , J Biol Chem201:689-696, 1953.
32.
Robertson, W.Van B.: Concentration of Collagen in Guinea Pig Tissues in Acute and Prolonged Scurvy, J Biol Chem187: 673-677, 1950.
33.
Kirchheiner, B.: Vitamin C Deficiency and Connective Tissue, Hexosamine, Hexuronic Acid and Hydroxyproline of Costal Cartilage and Intact Skin of Guinea Pig, Acta Rheum Scand11:71-80, 1965.
34.
Robertson , W.Van B.: The Effect of Ascorbic Acid Deficiency on the Collagen Concentration of Newly Induced Fibrous Tissue, J Biol Chem196:403-408, 1952.
