Delayed hypersensitivity reactions in guinea pig mucosa were studied by use of tritiated thymidine. No selective accumulation of labeled cells from regional nodes was found in the reaction sites, and no difference was found between test and control animals in the activity of the regional nodes. Cell proliferation in the mucosal reaction began early and the proportion of cells dividing increased with time.
Get full access to this article
View all access options for this article.
References
1.
Gell, P.G.H. , and Hinde, I.T.: The Histology of the Tuberculin Reaction and Its Modification by Cortisone, Brit J Exp Path32:516-529, 1951.
2.
Metataxas, M.M. , and Metataxax-Büh-Ler, M.: Passive Transfer of Local Cutaneous Hypersensitivity to Tuberculin, Proc Soc Exp Biol Med69:163-165, 1948.
3.
Najarian, J.S. , and Feldman, J.D.: Specificity of Passively Transferred Delayed Hypersensitivity, J Exp Med118:341-352, 1963.
4.
McCluskey, R.T. ; Benacerraf, B.; and McCluskey, J.W.: Studies on the Specificity of the Cellular Infiltrate in Delayed Hypersensitivity Reactions, J Immunol90: 466-477, 1963.
5.
Gowans, J.L. , and McGregor, D.D.: The Immunological Activities of Lymphocytes , Prog Allergy9:1-78, 1965.
6.
Bloom, B.R., and Bennett, B.: Mechanism of a Reaction In Vitro Associated with Delayed-Type Hypersensitivity , Science153: 80-82, 1966.
7.
Cronkite, E.P.; Bond, V.P.; Fliedner, T.M.; and Killman, S.V. : The Use of Tritiated Thymidine in the Study of Haemopoietic Cell Proliferation, in Wolstenholme, G.E.W., and O'Connor, Maeve (eds.), Haemopoiesis, London: Ciba Foundation Symposium, 1960, p. 70.
8.
Kosunen, T.V. ; Waksman, B.H.; Flau, M.H.; and Liken, W.S.: Radioautographic Study of Cellular Mechanisms in Delayed Hypersensitivity: I. Delayed Reactions to Tuberculin and Purified Proteins in the Rat and Guinea Pig, Immunology6:276-290, 1963.
9.
Spector, W.G. ; Walters, M.N.I.; and Willoughby , D.A.: The Origin of the Mononuclear Cells in Inflammatory Exudates Induced by Fibrinogen, J Path Bact90:181-192, 1965.
10.
Gillman, T. , and Wright, L.J.: Probable In Vivo Origin of Multinucleated Giant Cells from Circulating Mononuclears, Nature209:263-265, 1966.
11.
Volkman, A. , and GowANs, J.L.: The Production of Macrophages in the Rat , Brit J Exp Path46:50-61, 1965.
12.
Volkman, A. , and GowANs, J.L.: The Origin of Macrophages from Bone Marrow in the Rat, Brit J Exp Path46:62-70, 1965.
13.
Turk, J.L., and Stone, S.H.: Implications of the Cellular Changes in Lymph Nodes During the Development and Inhibition of Delayed Type Hypersensitivity, in Amos, B., and Koprowski, H., (eds.), Cell Bound Antibodies, Philadelphia: Wistar Institute Press, 1963, p 51.
14.
Landsteiner, K., and Chase, M.W.: Experiments on Transfer of Cutaneous Sensitivity to Simple Compounds, Proc Soc Exp Biol Med49:688-690, 1942.
15.
Chase, M.W. : The Cellular Transfer of Cutaneous Hypersensitivity to Tuberculin, Proc Soc Exp Biol Med59:134-135, 1945.
16.
Stavitsky, A.B.: Passive Cellular Transfer of the Tuberculin Type of Hypersensitivity, Proc Soc Exp Biol Med67:225-227, 1948.
17.
Lawrence, H.S. : The Cellular Transfer of Cutaneous Hypersensitivity to Tuberculin in Man, Proc Soc Exp Biol Med71 :516-522, 1949.
18.
Cohen, S.; Mccluskey, R.T.; and Benacerraf, B.: Studies of the Specificity of the Cellular Infiltrate of Delayed Hypersensitivity Reactions, J Immunol98:269-273, 1967.
19.
Turk, J.L., and OoRT, J.: A Histological Study of the Early Stages of the Development of the Tuberculin Reaction After Passive Transfer of Cells Labeled with (3H) Thymidine, Immunology6:140-147, 1963.
20.
Groth, 0.: The Selection of Lymphoid Cells in Allergic Contact Dermatitis of Guinea Pigs , Acta Dermatovener44:21-32, 1964.
21.
Liden, S.: Epithelial-Cell Reutilization of Nuclear Material and Selective Accumulation of Lymphoid Cells in Allergic Contact Dermatitis, Acta Dermatovener47:137-149, 1967.
22.
KosuNEN, T.U.: Origin and Specificity of Mononuclear Cells in Delayed Hypersensitivity Reactions, Nature211:1418-1419, 1966.
23.
Luden, S.: The Mononuclear-Cell Infiltrate in Allergic Contact Dermatitis: III. Selective Accumulation of Cells from Lymph Nodes, Acta Path Microbiol Scand, in press, 1969.
24.
Adams, D.; Williamson, J.J.; and Dolby, A.E.: Delayed Hypersensitivity Reaction in the Oral Mucosa of the Guinea Pig, JDent Res, in press, 1969.
25.
Bray, G.A.: A Simple Efficient Liquid Scintillator for Counting Aqueous Solutions in a Liquid Scintillation Counter, Anal Biochem1:279-285, 1960.
