Ultraviolet light-induced phosphorescence of collagen and globular protein in connective tissue was analyzed by spectral and kinetic studies. Computer resolution of the lifetimes of phosphorescent excited states of each protein, and their contribution to total luminous flux at zero time, constituted a means of simultaneous assessment of protein changes in cartilage, because of age, and because of regrowth of transplanted bone.
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References
1.
Tiede, E. , and Chomse, H.: Uber die Luminescenz die Zahne, Ber Dtsch Chem Ges67B:1988-1993, 1934 .
2.
Wisotzky, J. : Phosphorescence of Teeth Cooled in Liquid Nitrogen , JADA67 :392-396, 1963.
3.
Steele, R.H. , and Szent-Gyorgyi , A.: On Excitation of Biological Substances, Proc Nat Acad Sci43:477-491, 1957.
4.
Armstrong, W.G. , and Horsley, H.J.: Isolation of Fluorescent Components from Ox-Bone, Human Dentine and Gelatine, Nature211:981, 1966.
5.
Hoerman, K.C. , and Mancewicz, S.A.: Phosphorescence of Calcified Tissues, Arch Oral Biol9:517-534, 1964.
6.
Andersen, S.O. : Characterization of A New Type of Cross-Linkage in Resilin, A Rubber-Like Protein, Biochem Biophys Acta69:249-262, 1963.
7.
Teale, F.W.J. : The Ultraviolet Fluorescence of Proteins in Neutral Solution, Biochem J76:381-388, 1960.
8.
Vladmirov , LA, and Litvin, F.F.: Low Temperature Afterglow of Aromatic Amino Acids and Proteins, Biofizka5:127-133, 1960.
9.
Konev , S.V. : Fluorescence and Phosphorescence of Proteins and Nucleic Acids, New York: Plenum Press, 1967.
10.
Debye, P., and Edwards, J.O.: A Note on the Phosphorescence of Protein. Science116:143-144, 1952.
11.
Weber, G.: A Symposium on Light and Life, W. D. McElroy and Bentley Glass (eds.), Baltimore: John Hopkins University Press, 1961.
12.
Longworth, J.N.: Tyrosine Phosphorescence of Proteins, Biochem J81 :23P, 1961.
13.
Hoerman, K.C. , and Balekjian, A.Y.: Some Quantum Aspects of Collagen, Fed Proc25:1016-1021, 1966.
14.
Hoerman, K.C. ; Mancewicz, S.A.; and Balekjian , A.Y.: Low Temperature Afterglow Spectrums of Human Calcified Tissues, J Dent Res45:216, 1966.
15.
Eastoe, J.E.: Biochemists Handbook, Chemical Composition of Bone, Teeth and Cartilage, Cyril Long (ed.), Princeton: D. Van Nostrand Co. Inc.1961, pp 715-726.
16.
Boyd, G.E. : Method of Activation Analysis, Anal Chem21:335-347, 1949.
17.
St. John, P.A., and Winefordner , J.D.: Time Resolved Phosphorimetry as a Method of Simultaneous Analysis of Two-Component Mixtures, Anal Chem39:500-503, 1967.
18.
Boyne, P.J.: Textbook of Oral Surgery, Vol. 3, G. O. Kruger (ed.), St. Louis: C. V. Mosby Co., 1968.
19.
Van Duuren, B.L., and Bardi, C.E.: Reflectance Fluorescence Spectra of Aromatic Compounds in Potassium Bromide Pellets, Anal Chem35:2198-2202. 1963.
