Two micro-modifications are described for determination of hydroxyproline. These methods were used on ribbons of 5-μm histologic sections. Adjacent sections were projected and traced, and tracings were cut and weighed to determine the area of connective tissue. Quantitative chemical concentrations of collagen in histologic sections were determined.
Get full access to this article
View all access options for this article.
References
1.
Levy, M.: Studies on Enzymatic Histochemistry XVII: A Micro-Kjeldahl Estimation, Compt Rend Lab. Carlsberg21:101-110, 1956.
2.
Linderstrom-Lang, K.: "Distribution of Enzymes in Tissues and Cells," in The Harvey Lectures, Baltimore: Williams & Wilkins Co., 1939, pp 214-245.
3.
Linderstrom-Lang, K., and Holter, H.: Verteilung einiger Enzyme in den Schleimhaut-schichten des Magendarmkanales einiger vertebraten, Compt Rend Lab. Carlsberg23:135-148, 1940.
4.
Palmer, A.H., and Levy, M.: Chemistry of the Chick Embryo: III. Distribution of Dipeptidase in the Cephalic Region of the Three Day Embryo, J Biol Chem136:629, 1940.
5.
Stanton, G., and Levy, M.: Studies of Gingival Collagen, IADR Program and Abstracts of Papers, No. 421, 1966, p 145.
6.
Stegemann, H. : Microdetermination of Hydroxyproline with Chloramine-T and p-Dimethylaminobenzaldehyde, Z Physiol Chem311:41-45, 1958.
7.
Stanton, G.: A Study of Human Gingival Collagen, Dissert Abstr28:789B, 1967.
