Levan sucrase and hydrolase were present consistently in samples of dental plaque, but only the hydrolase was extractable with water. When plaque was incubated with sucrose, the levan content of the suspension reached a steady state that depended on the duration of incubation, sucrose concentration, and pH of the medium.
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References
1.
Snyder, M.L. ; Hackedorn, H.M.; Martin, D.O.; and Johnston , D.D.: The Synthesis of Mucinous Polysaccharides from Sucrose by Oral Bacteria, J Dent Res34 :368-379, 1955.
2.
Avigad, G.: "Levan," in WHISTLER, R.L., (ed.) : Methods in Carbohydrate Chemistry, New York: Academic Press, 1965, vol 5, p 162.
3.
Wood, J.M.: The Amount, Distribution, and Metabolism of Soluble Polysaccharides in Human Dental Plaque, Arch Oral Biol12:849-858, 1967.
4.
Manly, R.S.: Retention of Carbohydrate from Sugar Solutions by Salivary Sediment, J Dent Res40:379, 1961.
5.
Manly, R.S., and Dain, J.A.: Conversion of Sucrose to Polysaccharide by Oral Samples, abstracted, J Dent Res40:747, 1961.
6.
Dain, J.A., and Manly, R.S.: The Identity of the Polysaccharide Produced by Oral Samples from Sucrose Solutions, abstracted, J Dent Res40:720, 1961.
7.
Manly, R.S.: Plaque Measurements, Proc NY Acad Sci, in press.
8.
McDougall, W.A.: Dental Plaque: IV. Levan of the Dental Plaque, Aust Dent J9:1-5, 1964.
9.
Wood, J.M., and Critchley, P.: The Extracellular Polysaccharide Produced from Sucrose by a Cariogenic Steptococcus , Arch Oral Biol11:1039-1042, 1966 .
10.
Howell, JR., A., and Jordan, H.V.: Production of an Extracellular Levan by Odontomyces viscosus, Arch Oral Biol12: 571-573, 1967.
11.
Manly, R.S.; Liberfarb, R.; Cormier, A.; and O'Brien, A.: Automated Analysis of Levan Content and Formation Rate in Dental Plaque, read before 1966 Technicon Conference, Ardsley, NY, Technicon Instruments, 1967.
12.
Niven, C.F. , JR. ; Smiley, K.L.; and Sherman, J.M.: The Production of Large Amounts of a Polysaccharide by Streptococcus salivarius, J Bact41:479-484, 1941.
13.
Newbrun, E. , and Baker, S.: PhysicoChemical Characteristics of the Levan Produced by Streptococcus salivarius, Carbohyd Res6:165-170, 1968.
14.
Gibbons, R.J. , and Banghart, S.B.: Synthesis of Extracellular Dextran by Cariogenic Bacteria and Its Presence in Human Dental Plaque, Arch Oral Biol12:11-24, 1967.
15.
Koulourides, T.: Experimental Changes of Enamel Mineral Density, read before Conference on the Art and Science of Dental Caries Research. Massachusetts Institute of Technology. Cambridge, Mass. Sept 26-27. 1966.
16.
Dedonder, R.: ''Levan sucrase from Bacillus subtilis," in COLOWICH, S.P., and KAP-LAN, N .O. (eds.) : Methods in Enzymology, New York: Academic Press, 1966, vol 8, p 500.
17.
Gibbons, R.J. , and Socransky, S.S.: Intracellular Polysaccharide Storage by Organisms in Dental Plaques, Arch Oral Biol7:73-80, 1962.
18.
Vanhoute, J. : Relationship between Carbohydrate Intake and Polysaccharide-Storing Micro-Organisms in Dental Plaque, Arch Oral Biol9:91-93, 1964.
19.
Herscowitz, H.B., and Hammond, B.F.: Presence of Polysaccharide-Forming Coliforms in Human Saliva, J Dent Res44:617, 1965.
