Bacteria were found in histologic sections and cultures of unexposed pulp in 48 of 67 teeth from dogs. Deep Class V cavities had been prepared in these teeth. The dogs had been intravenously inoculated with bacteria of the same immunologic types as those identified in pulps.
Get full access to this article
View all access options for this article.
References
1.
Boling, L.R. , and Robinson, H.B.G.: Vascular Changes in Inflamed Dental Pulp, abstracted, J Dent Res17:310, 1938.
2.
Kakehashi, S. ; Stanley, H.R.; and Fitz-Gerald , R.J.: The Effects of Surgical Exposure of Dental Pulps in Germ-free and Conventional Laboratory Rats, Oral Surg20:340-344, 1965.
3.
Winkler, K.C. , and Van Amerongen , J.: Bacteriologic Results from 4,000 Root Canal Cultures, Oral Surg12:857-875, 1959.
4.
Burnett, G.W. , and Scherp, H.W.: Oral Microbiology and Infectious Disease , Baltimore : Williams & Wilkins Co., 1957, pp 333-335.
5.
Dutton, E.M. , and Cameron, G.C.: Dental Infection; Culture of Pulps from 275 Extracted Vital Teeth, Canad J Public Health23:436, 1932.
Macdonald, J.B. ; Hare, G.D. ; and Wood, A.W.S.: Bacteriological Status of the Pulp Chambers of Intact Teeth Found to be Nonvital Following Trauma, Oral Surg10:318-322, 1957.
8.
Tunnicliff, R., and Hammond, C.: Presence of Bacteria in Pulps of Intact Teeth , J Amer Dent Assoc24:1663-1666, 1937 .
9.
Fox, J.P. : The Localization and Concentration of Blood-Borne Antibodies and Colloidal Dye in Areas of Inflammation of Various Ages, J Immun31:293-308, 1936.
10.
Rigdon, R.H.: Localization of Staphylococci in Areas of Inflammation Produced by Xylene , Arch Surg41:879-887, 1940.
Macintosh, R.B. : Study of Anachoresis in Mice Utilizing Serratia marcescens, abstracted, J Dent Res37:43, 1958.
13.
Cameron, G.C. ; Rae, C.A.; and Murphy, G.N.: Blood Cultures and Focal Infection: An Experimental Study with 100 Healthy Adults, Canad Med Assoc J25:131-134. 1931.
14.
Burket, L.W., and Burn, C.G.: Bacteremias Following Dental Extractions, J Dent Res16:521-530, 1937.
15.
Robinson, H.B.G. , and Boling, L.R.: The Anachoretic Effect in Pulpitis, I. Bacteriologic Studies, J Amer Dent Assoc38 :268-282, 1941.
16.
Boling, L.R. , and Robinson, H.B.G.: The Anachoretic Effect in Pulpitis: II . Histologic Studies, Arch Oral Path33:477-486, 1942.
17.
Burke, G.W. , JR., and Knighton , H.T.: The Incidence of Microorganisms in Inflamed Dental Pulps of Rats Following Bacteremia, J Dent Res39:205-214, 1960.
18.
Smith, L., and Tappe, G.D.: Experimental Pulpitis in Rats, J Dent Res47:17-22, 1962.
19.
Miller, M.E.; Christensen, G.C.; and Evans, H.E.: Anatomy of the Dog, Philadelphia : W. B. Saunders Co., 1964. pp 649-654.
20.
Brown, J.H. , and Brenn, L.: A Method for the Differential Staining of Gram Positive and Gram Negative Bacteria in Tissue Sections, Bull Hopkins Hosp48:69-73, 1931.
21.
Taylor, R.D. : Modification of the Brown and Brenn Gram Stain for the Differential Staining of Gram Positive and Gram Negative Bacteria in Tissue Sections. Amer J Clin Path46:472-474, 1966.
22.
Mitchell, D.F. : The Irritational Qualities of Dental Materials , J Amer Dent Assoc59:954-966, 1959.
23.
Mitchell, D.F. ; Buonocore, M.G.; and Shazer, S.: Pulp Reaction to Silicate Cement and Other Materials: Relation to Cavity Depth. J Dent Res41:591-595, 1962.
24.
Van Huysen, G., and Gurley, W.B.: Histologic Changes in the Teeth of Dogs Following Preparation of Cavities of Various Depths and Their Exposure to Oral Fluids, J Amer Dent Assoc26:87-100. 1939.
25.
El-Kafrawy, A.H., and Mitchell , D.F.: Pulp Reactions to Open Cavities Later Restored with Silicate Cement. J Dent Res42:874-884, 1963.
26.
Lutsky, I., and Farmer, S.G.: A Re-evaluation of the Assumed Sterility of Canine Blood. Lab Anim Care16:472-475, 1966.
27.
Wagner, R.P. , and Kruger, G.O.: Bacteriological Investigations of Bacteremia Following Restorative Dental Procedures Utilizing Blood Sampling Techniques , IADR Preprinted Abstracts, 1963.
28.
Grossman, L.I. : Minimum Number of Microorganisms Needed to Initiate Growth in Culture Media. J Dent Res45:81-85, 1966.