Tooth germs grown on Eagle's medium solidified with agar will differentiate in vitro. Normal development was suppressed by beta-2-thienylalanine (1 or 2 millimols per liter), but it proceeded when phenylalanine and beta-2-thienylalanine were present in equimolar concentrations. This effect may have been due to interference with protein metabolism in the dermal papillar cells.
Get full access to this article
View all access options for this article.
References
1.
Billingham, R.E., and Silvers, W.K.: The Origin and Conservation of Epidermal Specificities, New Eng J Med268:477-480, 1963.
2.
Glasstone, S.: The Development of Tooth Germs In Vitro, J Anat70:260-266, 1936.
3.
Lefkowitz, W.; Bodecker, C.F.; and Mardfin, D.F.: Odontogenesis of the Rat Molar; Prenatal Stage, J Dent Res32 :749-772, 1953.
4.
Hay, M.F. : The Development In Vivo and In Vitro of the Lower Incisors and Molars of the Mouse, Arch Oral Biol3:86-109, 1961.
5.
Glasstone, S. : Regulative Changes in Tooth Germs Grown in Tissue Culture, J Dent Res42:1364-1368, 1963.
6.
Koch, W.E. : In Vitro Development of Tooth Rudiments of Embryonic Mice, Anat Rec152:513-524, 1965.
7.
Glasstone, S. : Morphodifferentiation of Teeth in Embryonic Mandibular Segments in Tissue Culture, J Dent Res46:611-614, 1967.
8.
Huggins, C.B. ; Mccarroll, H.R.; and Dahlberg , A.A.: Transplantation of Tooth Germ Elements and the Experimental Heterotopic Formation of Dentin and Enamel, J Exp Med60:199-210, 1934.
9.
Koch, W.E.: In Vitro Differentiation of Tooth Rudiments of Embryonic Mice: I. Transfilter Interaction of Embryonic Incisor Tissues, J Exp Zool165:155-170, 1967.
10.
Kollar, E.J. : An In Vitro Study of Hair and Vibrissae Development in Embryonic Mouse Skin, J Invest Derm46:254-262, 1966.
11.
Cohen, J.: The Transplantation of Individual Rat and Guinea-pig Whisker Papillae, J Embryol Exp Morph9:117-127, 1961.
12.
Hardy, M.H. : The Development of Mouse Hair In Vitro with Some Observations on Pigmentation, J Anat83:364-384, 1949.
13.
Davidson, P. , and Hardy, M.H.: The Development of Mouse Vibrissae In Vivo and In Vitro, J Anat86:342-356, 1952.
14.
Gruneberg, H.: The Development of Some External Features in Mouse Embryos, J Hered34:89-92, 1943.
15.
Lefkowitz, W., and Swayne, P.: Normal Development of Tooth Buds Cultured In Vitro, J Dent Res37:1100-1114, 1958 .
16.
Rabinowitz, M.; Olson, M.E.; and Green-Berg , D.M.: Independent Antagonism of Amino Acid Incorporation into Proteins, J Biol Chem210:837-849, 1954.
17.
Savchuck, W.B. ; Merriman, M.E.; and Lockhart , W.L.: Assay of Vitamins and Amino Acids with Cultured Tissue Cells and Antimetabolites, Appl Microbiol12: 316-320, 1964.
18.
Hruban, Z.; Villa-Trevino, S.; and Farber, E.: Effects of Some Phenylalanine Analogs upon Protein Metabolism In Vivo, Lab Invest14:468-474, 1965.
19.
Caviness, V.S. , JR.: The Effect of Beta-2-thienylalanine upon RNA Synthesis in the Macrophage, Exp Cell Res44:234-240, 1966.
20.
Caviness, V.S. , JR.: The Effect of Beta-2-thienylalanine upon Protein Synthesis in the Macrophage, Exp Cell Res44:287-294, 1966.
21.
Kollar, E.J. : The Inhibition of Vibrissae Development In Vitro by Beta-2-thienylalanine, J Invest Derm, to be published.
22.
Wilde, C.E. , JR.: The Urodele Neuroepithelium : II. The Relationship Between Phenyl Alanine Metabolism and the Differentiation of Neural Crest Cells, J Morph97:313-344, 1955.
23.
Pourtois, M. : Comportement en Culture In Vitro des Ebauches Dentaires de Rongeurs Prelevees aux Stades de Predifferenciation, J Embryol Exp Morph12:391-405, 1964.
