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Abstract

The aim of this study was to investigate the response of human periodontal ligament (hPDL) fibroblasts to an intermittent compressive force and its effect on the expression of SOST, POSTN, and TGFB1. A computerized cell compressive force loading apparatus was introduced, and hPDL cells were subjected to intermittent compressive force. The changes in messenger RNA (mRNA) and protein expression were monitored by real-time polymerase chain reaction and Western blot analysis, respectively. An increased expression of SOST, POSTN, and TGFB1 was observed in a time-dependent fashion. Addition of cycloheximide, a transforming growth factor (TGF)–β inhibitor (SB431542), or a neutralizing antibody against TGF-β1 attenuated the force-induced expression of SOST and POSTN as well as sclerostin and periostin, indicating a role of TGF-β1 in the pressure-induced expression of these proteins. Enzyme-linked immunosorbent assay analysis revealed an increased level of TGF-β1 in the cell extracts but not in the medium, suggesting that intermittent compressive force promoted the accumulation of TGF-β1 in the cells or their surrounding matrix. In conclusion, an intermittent compressive force regulates SOST/POSTN expression by hPDL cells via the TGF-β1 signaling pathway. Since these proteins play important roles in the homeostasis of the periodontal tissue, our results indicate the importance of masticatory forces in this process.

Keywords

intermittent compressive force periodontal ligament cells sclerostin periostin transforming growth factor β1 mechanotransduction

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References

Afanador

Yokozeki

Oba

Kitase

Takahashi

Kudo

Moriyama

. 2005. Messenger RNA expression of periostin and Twist transiently decrease by occlusal hypofunction in mouse periodontal ligament. Arch Oral Biol. 50(12):1023–1031.

Andreasen

Kristerson

. 1981. The effect of limited drying or removal of the periodontal ligament: periodontal healing after replantation of mature permanent incisors in monkeys. Acta Odontol Scand. 39(1):1–13.

Arnsdorf

Tummala

Jacobs

. 2009. Non-canonical Wnt signaling and N-cadherin related beta-catenin signaling play a role in mechanically induced osteogenic cell fate. PLoS One. 4(4):e5388.

Burger

Klein-Nulend

van der Plas

Nijweide

. 1995. Function of osteocytes in bone—their role in mechanotransduction. J Nutr. 125(7 Suppl):2020S-2023S.

Chien

. 2007. Mechanotransduction and endothelial cell homeostasis: the wisdom of the cell. Am J Physiol Heart Circ Physiol. 292(3):H1209–H1224.

Docheva

Padula

Popov

Weishaupt

Prägert

Miosge

Hickel

Böcker

Clausen-Schaumann

Schieker

. 2010. Establishment of immortalized periodontal ligament progenitor cell line and its behavioural analysis on smooth and rough titanium surface. Eur Cell Mater. 19:228–241.

Duncan

Turner

. 1995. Mechanotransduction and the functional response of bone to mechanical strain. Calcif Tissue Int. 57(5):344–358.

Fill

Toogood

Major

Carey

. 2012. Analytically determined mechanical properties of, and models for the periodontal ligament: critical review of literature. J Biomech. 45(1):9–16.

Horiuchi

Amizuka

Takeshita

Takamatsu

Katsuura

Ozawa

Toyama

Bonewald

Kudo

. 1999. Identification and characterization of a novel protein, periostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor beta. J Bone Miner Res. 14(7):1239–1249.

10.

Kuchler

Schwarze

Dobsak

Heimel

Bosshardt

Kneissel

Gruber

. 2014. Dental and periodontal phenotype in sclerostin knockout mice. Int J Oral Sci. 6(2):70–76.

11.

Lehnen

Gotz

Baxmann

Jager

. 2012. Immunohistochemical evidence for sclerostin during cementogenesis in mice. Ann Anat. 194(5):415–421.

12.

Lindskog

Pierce

Blomlof

Hammarstrom

. 1985. The role of the necrotic periodontal membrane in cementum resorption and ankylosis. Endod Dent Traumatol. 1(3):96–101.

13.

Loots

Keller

Leupin

Murugesh

Collette

Genetos

. 2012. TGF-beta regulates sclerostin expression via the ECR5 enhancer. Bone. 50(3):663–669.

14.

Luckprom

Kanjanamekanant

Pavasant

. 2011. Role of connexin43 hemichannels in mechanical stress–induced ATP release in human periodontal ligament cells. J Periodontal Res. 46(5):607–615.

15.

Luckprom

Wongkhantee

Yongchaitrakul

Pavasant

. 2010. Adenosine triphosphate stimulates RANKL expression through P2Y1 receptor-cyclo-oxygenase-dependent pathway in human periodontal ligament cells. J Periodontal Res. 45(3):404–411.

16.

Liu

Cui

Hasegawa

Hongo

Feng

Sun

Kudo

Amizuka

. 2014. Histochemical examination of cathepsin K, MMP1 and MMP2 in compressed periodontal ligament during orthodontic tooth movement in periostin deficient mice. J Mol Histol. 45(3):303–309.

17.

Naka

Yokose

. 2011. Spatiotemporal expression of sclerostin in odontoblasts during embryonic mouse tooth morphogenesis. J Endod. 37(3):340–345.

18.

Nguyen

Tang

Nguyen

Alliston

. 2013. Load regulates bone formation and sclerostin expression through a TGFbeta-dependent mechanism. PLoS One. 8(1):e53813.

19.

Nishimura

. 2009. Integrin-mediated transforming growth factor–beta activation, a potential therapeutic target in fibrogenic disorders. Am J Pathol. 175(4):1362–1370.

20.

Pattamapun

Tiranathanagul

Yongchaitrakul

Kuwatanasuchat

Pavasant

. 2003. Activation of MMP-2 by Porphyromonas gingivalis in human periodontal ligament cells. J Periodontal Res. 38(2):115–121.

21.

Pavasant

Yongchaitrakul

. 2011. Role of mechanical stress on the function of periodontal ligament cells. Periodontol 2000. 56(1):154–165.

22.

Rios

Xie

Giannobile

Bonewald

Conway

Feng

. 2008. Periostin is essential for the integrity and function of the periodontal ligament during occlusal loading in mice. J Periodontol. 79(8):1480–1490.

23.

Robling

Niziolek

Baldridge

Condon

Allen

Alam

Mantila

Gluhak-Heinrich

Bellido

Harris

. 2008. Mechanical stimulation of bone in vivo reduces osteocyte expression of Sost/sclerostin. J Biol Chem. 283(9):5866–5875.

24.

Romanos

Asnani

Hingorani

Deshmukh

. 2014. PERIOSTIN: role in formation and maintenance of dental tissues. J Cell Physiol. 229(1):1–5.

25.

Saito

Salmon

Amorim

Ambrosano

Casati

Sallum

Nociti

Silvério

. 2014. Characterization of highly osteoblast/cementoblast cell clones from a CD105-enriched periodontal ligament progenitor cell population. J Periodontol. 85(6):e205–211.

26.

Semenov

Tamai

. 2005. SOST is a ligand for LRP5/LRP6 and a Wnt signaling inhibitor. J Biol Chem. 280(29):26770–26775.

27.

Shiga

Kapila

Zhang

Hayami

Kapila

. 2003. Ascorbic acid induces collagenase-1 in human periodontal ligament cells but not in MC3T3-E1 osteoblast-like cells: potential association between collagenase expression and changes in alkaline phosphatase phenotype. J Bone Miner Res. 18(1):67–77.

28.

Tabata

Hongo

Sasaki

Hasegawa

de Freitas

Yamada

Yamamoto

Suzuki

Yamamoto

Oda

. 2014. Altered distribution of extracellular matrix proteins in the periodontal ligament of periostin-deficient mice. Histol Histopathol. 29(6):731–742.

29.

Rhee

Condon

Bivi

Allen

Dwyer

Stolina

Turner

Robling

Plotkin

. 2012. Sost downregulation and local Wnt signaling are required for the osteogenic response to mechanical loading. Bone. 50(1):209–217.

30.

Wen

Chau

Jackson-Boeters

Elliott

Daley

Hamilton

. 2010. TGF-β1 and FAK regulate periostin expression in PDL fibroblasts. J Dent Res. 89(12):1439–1443.

31.

Wilde

Yokozeki

Terai

Kudo

Moriyama

. 2003. The divergent expression of periostin mRNA in the periodontal ligament during experimental tooth movement. Cell Tissue Res. 312(3):345–351.

32.

Wongkhantee

Yongchaitrakul

Pavasant

. 2008. Mechanical stress induces osteopontin via ATP/P2Y1 in periodontal cells. J Dent Res. 87(6):564–568.

33.

Tay

Leong

Tan

Liao

Tan

. 2010. Mechanical behavior of human mesenchymal stem cells during adipogenic and osteogenic differentiation. Biochem Biophys Res Commun. 393(1):150–155.

34.

Zhu

Hsu

Hollis

. 2014. Increased number of chews during a fixed–amount meal suppresses postprandial appetite and modulates glycemic response in older males. Physiol Behav. 133:136–140.

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Mechanical Force–induced TGFB1 Increases Expression of SOST/POSTN by hPDL Cells

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