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Abstract

The effect of chronic fluoride (F) exposure from the drinking water on parameters related to glucose homeostasis was investigated. Wistar rats were randomly distributed into 2 groups (diabetic [D] and nondiabetic [ND]; n = 54 each). In D, diabetes was induced with streptozotocin. Each group was further divided into 3 subgroups (0, 10, or 50 mgF/L in drinking water). After 22 days of treatment, plasma and liver samples were collected. No alterations in glycemia, insulinemia, K_ITT, and HOMA2-IR (homeostasis model assessment 2 of insulin resistance) were seen for ND. F-exposure of D rats led to significantly lower insulinemia, without alterations in glycemia (increased %S). Proteomic analysis detected 19, 39, and 16 proteins differentially expressed for the comparisons D0 vs. D10, D0 vs. D50, and D10 vs. D50, respectively. Gene Ontology with the most significant terms in the comparisons D0 vs. D10, D0 vs. D50, and D50 vs. D10 were organic acid metabolic process and carboxylic acid metabolic process, organic acid metabolic process, and cellular ketone metabolic process. Analysis of subnetworks revealed that proteins with fold changes interacted with GLUT4 in comparison D0 vs. D10. Among these proteins, ERj3p was present in D10. Upregulation of this protein in the presence of F might help to explain the higher %S found in these animals. These data suggest that fluoride might enhance glucose homeostasis in diabetes and identify specific biological mechanisms that merit future studies.

Keywords

water fluoridation proteomics liver glucose intolerance glucose

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References

Ates

Cayli

Yucel

Altinoz

Kocak

Durak

Turkoz

Yologlu

. 2007. Central nervous system protection by resveratrol in streptozotocin-induced diabetic rats. J Clin Neurosci. 14(3):256–260.

Bauer-Mehren

. 2013. Integration of genomic information with biological networks using Cytoscape. Methods Mol Biol. 1021:37–61.

Bindea

Galon

Mlecnik

. 2013. CluePedia Cytoscape plugin: pathway insights using integrated experimental and in silico data. Bioinformatics. 29(5):661–663.

Bindea

Mlecnik

Hackl

Charoentong

Tosolini

Kirilovsky

Fridman

Pagès

Trajanoski

Galon

. 2009. ClueGO: a Cytoscape plug-in to decipher functionally grouped gene ontology and pathway annotation networks. Bioinformatics. 25(8):1091–1093.

Bonora

Moghetti

Zancanaro

Cigolini

Querena

Cacciatori

Corgnati

Muggeo

. 1989. Estimates of in vivo insulin action in man: comparison of insulin tolerance tests with euglycemic and hyperglycemic glucose clamp studies. J Clin Endocrinol Metab. 68(2):374–378.

Bradford

. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72:248–254.

Buzalaf

Levy

. 2011. Fluoride intake of children: considerations for dental caries and dental fluorosis. Monogr Oral Sci. 22:1–19.

Buzalaf

Whitford

. 2011. Fluoride metabolism. Monogr Oral Sci. 22:20–36.

Chiba

Colombo

Shirakashi

da Silva

Saliba

Moimaz SA

Saliba

Garbin CA

Antoniali

Sumida

. 2012. NaF treatment increases TNF-a and resistin concentrations and reduces insulin signal in rats. J Fluorine Chem. 136:3–7.

10.

Chiba

Colombo

Shirakashi

de Souza

Gomes WD

Saliba

Moimaz SA

Saliba

Garbin CA

Silva

Sumida

. 2010. Insulin signal decreases in muscle but not in the liver of castrated male rats from chronic exposure to fluoride. Fluoride. 43(1):25–30.

11.

Clayton

Collins

. 2014. Policy: NIH to balance sex in cell and animal studies. Nature. 509(7500): 282–283.

12.

de la Sota

Puche

Rigalli

Fernández

Benassati

Boland

. 1997. Changes in bone mass and in glucose homeostasis in subjects with high spontaneous fluoride intake. Medicina (B Aires). 57(4):417–420.

13.

Ding

Song

Malik

Liu

. 2006. Sex differences of endogenous sex hormones and risk of type 2 diabetes: a systematic review and meta-analysis. JAMA. 295(11):1288–1299.

14.

Dunipace

Brizendine

Zhang

Wilson

Miller

Katz

Warrick

Stookey

. 1995. Effect of aging on animal response to chronic fluoride exposure. J Dent Res. 74(1):358–368.

15.

Kammoun

Chabanon

Hainault

Luquet

Magnan

Koike

Ferré

Foufelle

. 2009. GRP78 expression inhibits insulin and ER stress–induced SREBP-1c activation and reduces hepatic steatosis in mice. J Clinl Investig. 119(5):1201–1215.

16.

Komorowsky

Brosius

III Pennathur

Kretzler

. 2012. Perspectives on systems biology applications in diabetic kidney disease. J Cardiovasc Translat Res. 5(4):491–508.

17.

Leite

Gualiume Vaz Madureira

Lobo J

Barbosa da Silva

Pereira HA

Silva

Fernandes M

Martini

Zucki

Sumida

Rigalli

Buzalaf

. 2014. Proteomic analysis of gastrocnemius muscle in rats with streptozotocin-induced diabetes and chronically exposed to fluoride. PLoS One. 9(9):e106646.

18.

Levy

Matthews

Hermans

. 1998. Correct homeostasis model assessment (HOMA) evaluation uses the computer program. Diabetes Care. 21(12):2191–2192.

19.

Lupo

Buzalaf

Rigalli

. 2011. Effect of fluoridated water on plasma insulin levels and glucose homeostasis in rats with renal deficiency. Biol Trace Element Res. 140(2):198–207.

20.

Meisinger

Thorand

Schneider

Stieber

Doring

Lowel

. 2002. Sex differences in risk factors for incident type 2 diabetes mellitus: the MONICA Augsburg cohort study. Arch Intern Med. 162(1):82–89.

21.

Millan

. 2013. Visualization and analysis of biological networks. Methods Mol Biol. 1021:63–88.

22.

Nakatsuka

Wada

Iseda

Teshigawara

Higashio

Murakami

Kanzaki

Inoue

Terami

Katayama

. 2012. Vaspin is an adipokine ameliorating ER stress in obesity as a ligand for cell-surface GRP78/MTJ-1 complex. Diabetes. 61(11):2823–2832.

23.

Njolstad

Arnesen

Lund-Larsen

. 1998. Sex differences in risk factors for clinical diabetes mellitus in a general population: a 12-year follow-up of the Finnmark Study. Am J Epidemiol. 147(1):49–58.

24.

Nordquist

Sjoquist

. 2009. Improvement of insulin response in the streptozotocin model of insulin-dependent diabetes mellitus: insulin response with and without a long-acting insulin treatment. Animal. 3(5):685–689.

25.

Orchard

. 2012. Molecular interaction databases. Proteomics. 12(10):1656–1662.

26.

Ostrowski

Kawata

Schullery

Denisenko

Higaki

Abrass

Bomsztyk

. 2001. Insulin alters heterogeneous nuclear ribonucleoprotein K protein binding to DNA and RNA. Proc Natl Acad Sci USA. 98(16):9044–9049.

27.

Pereira

Leite

Ade L

Charone

Lobo

Cestari

Peres-Buzalaf

Buzalaf

. 2013. Proteomic analysis of liver in rats chronically exposed to fluoride. PLoS One. 8(9):e75343.

28.

Pessan

Toumba

Buzalaf

. 2011. Topical use of fluorides for caries control. Monogr Oral Sci. 22:115–132.

29.

Petersen

Dufour

Savage

Bilz

Solomon

Yonemitsu

Cline

Befroy

Zemany

Kahn

. 2007. The role of skeletal muscle insulin resistance in the pathogenesis of the metabolic syndrome. Proc Natl Acad Sci USA. 104(31):12587–12594.

30.

Ramires

Maia

Rigolizzo

Ddos S

Lauris

Buzalaf

. 2006. External control over the fluoridation of the public water supply in Bauru, SP, Brazil. Rev Saude Publ. 40(5):883–889.

31.

Richter

Hargreaves

. 2013. Exercise, GLUT4, and skeletal muscle glucose uptake. Physiol Rev. 93(3):993–1017.

32.

Rigalli

Alloatti

Menoyo

Puche

. 1995. Comparative study of the effect of sodium fluoride and sodium monofluorophosphate on glucose homeostasis in the rat. Arzneimittelforschung. 45(3):289–292.

33.

Rigalli

Ballina

Roveri

Puche

. 1990. Inhibitory effect of fluoride on the secretion of insulin. Calcif Tiss Int. 46(5):333–338.

34.

Taves

. 1968. Separation of fluoride by rapid diffusion using hexamethyldisiloxane. Talanta. 15(9):969–974.

35.

Thorand

Baumert

Kolb

Meisinger

Chambless

Koenig

Herder

. 2007. Sex differences in the prediction of type 2 diabetes by inflammatory markers: results from the MONICA/KORA Augsburg case-cohort study, 1984–2002. Diabetes Care. 30(4):854–860.

36.

Trivedi

Mithal

Gupta

Godbole

. 1993. Reversible impairment of glucose tolerance in patients with endemic fluorosis. Fluoride Collaborative Study Group. Diabetologia. 36(9):826–828.

37.

Willett

Dietz

Colditz

. 1999. Guidelines for healthy weight. N Engl J Med. 341(6):427–434.

38.

Yang

Zhang

Wang

Liu

. 2010. Insulin stimulates osteoblast proliferation and differentiation through ERK and PI3K in MG-63 cells. Cell Biochem Funct. 28(4):334–341.

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Low-Level Fluoride Exposure Increases Insulin Sensitivity in Experimental Diabetes

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