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Abstract

Bone marrow–derived mesenchymal stem/progenitor cells (BMSCs) are commonly used in regeneration therapy. The current primary source of BMSCs is the iliac crest; however, the procedure is associated with various burdens on the patient, including the risk of pain and infection. Hence, the possibility to collect BMSCs from other, more accessible, sources would be an attractive approach. It is well known that stem cells migrate from surrounding tissues and play important roles in wound healing. We thus hypothesized that stem/progenitor cells could be isolated from granulation tissue in the dental socket, and we subsequently collected granulation tissue from dog dental socket 3 d after tooth extraction. After enzyme digestion of the collected tissue, the cells forming colonies constituted the dental socket–derived stem/progenitor cells (dDSCs). Next, dDSCs were compared with dog BMSCs (dBMSCs) for phenotype characterization. A flow cytometric analysis showed that dDSCs were positive for CD44, CD90, and CD271 but negative for CD34 and CD45, similar to dBMSCs. dDSCs also exhibited osteogenic, adipogenic, and chondrogenic differentiation ability, similar to dBMSCs, with a higher capacity for colony formation, proliferation, and motility than dBMSCs. In addition, an in vivo ectopic bone formation assay showed that dDSCs and dBMSCs both induced hard tissue formation, although only dDSCs formed a fibrous tissue-like structure connected to the newly formed bone. Finally, we tested the ability of dDSCs to regenerate periodontal tissue in a one-wall defect model. The defects in the dDSC-transplanted group (β-TCP/PGA/dDSCs) were regenerated with cementum-like and periodontal ligament-like tissues and alveolar bone, whereas only bony tissue was observed in the control group (β-TCP/PGA). In conclusion, we identified and characterized a population of stem/progenitor cells in granulation tissue obtained from the dental socket that exhibited several characteristics similar to those of BMSCs. Dental sockets could therefore be a novel source for isolating stem/progenitor cells from bone.

Keywords

stem cells granulation tissue dental socket bone marrow–derived stem cells wound healing dental tissue–derived mesenchymal stem cells

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References

Alge

Zhou

Adams

Wyss

Shadday

Woods

. (2010). Donor-matched comparison of dental pulp stem cells and bone marrow-derived mesenchymal stem cells in a rat model. J Tissue Eng Regen Med 4:73-81.

Carvalho

Ramalho-Santos

(2013). How can ethics relate to science? The case of stem cell research. Eur J Hum Genet 21:591-595.

Catacchio

Berardi

Reale

De Luisi

Racanelli

Vacca

. (2013). Evidence for bone marrow adult stem cell plasticity: properties, molecular mechanisms, negative aspects, and clinical applications of hematopoietic and mesenchymal stem cells transdifferentiation. Stem Cells Int 2013:589139.

Evans

Kaufman

(1981). Establishment in culture of pluripotential cells from mouse embryos. Nature 292:154-156.

Friedenstein

Gorskaja

Kulagina

(1976). Fibroblast precursors in normal and irradiated mouse hematopoietic organs. Exp Hematol 4:267-274.

Hara

Ono

Eguchi

Kubota

Pham

Sonoyama

. (2013a). miRNA-720 controls stem cell phenotype, proliferation and differentiation of human dental pulp cells. PLoS One 8:e83545.

Hara

Ono

Kubota

Sonoyama

Oida

Hattori

. (2013b). Novel chondrogenic and chondroprotective effects of the natural compound harmine. Biochimie 95:374-381.

Iwata

Yamato

Tsuchioka

Takagi

Mukobata

Washio

. (2009). Periodontal regeneration with multi-layered periodontal ligament-derived cell sheets in a canine model. Biomaterials 30:2716-2723.

Karp

Leng Teo

(2009). Mesenchymal stem cell homing: the devil is in the details. Cell Stem Cell 4:206-216.

10.

Kawamoto

Shimizu

(2000). A method for preparing 2- to 50-micron-thick fresh-frozen sections of large samples and undecalcified hard tissues. Histochem Cell Biol 113:331-339.

11.

Klein

Simons

(2011). Universal patterns of stem cell fate in cycling adult tissues. Development 138:3103-3111.

12.

Martin

(1981). Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc Natl Acad Sci U S A 78:7634-7638.

13.

Mazzini

Mareschi

Ferrero

Vassallo

Oliveri

Boccaletti

. (2006). Autologous mesenchymal stem cells: clinical applications in amyotrophic lateral sclerosis. Neurol Res 28:523-526.

14.

Moviglia

Fernandez Vina

Brizuela

Saslavsky

Vrsalovic

Varela

. (2006). Combined protocol of cell therapy for chronic spinal cord injury: report on the electrical and functional recovery of two patients. Cytotherapy 8:202-209.

15.

Boucher

Koh

Sastry

Chase

Lakshmipathy

. (2008). PDGF, TGF-beta, and FGF signaling is important for differentiation and growth of mesenchymal stem cells (MSCs): transcriptional profiling can identify markers and signaling pathways important in differentiation of MSCs into adipogenic, chondrogenic, and osteogenic lineages. Blood 112:295-307.

16.

Ono

Kubota

Fujisawa

Sonoyama

Kawaki

Akiyama

. (2008). Promotion of hydroxyapatite-associated, stem cell-based bone regeneration by CCN2. Cell Transplant 17:231-240.

17.

Ono

Inkson

Kilts

Young

(2011). WISP-1/CCN4 regulates osteogenesis by enhancing BMP-2 activity. J Bone Miner Res 26:193-208.

18.

Pittenger

Mackay

Beck

Jaiswal

Douglas

Mosca

. (1999). Multilineage potential of adult human mesenchymal stem cells. Science 284:143-147.

19.

Sasaki

Abe

Fujita

Ando

Inokuma

Shimizu

(2008). Mesenchymal stem cells are recruited into wounded skin and contribute to wound repair by transdifferentiation into multiple skin cell type. J Immunol 180:2581-2587.

20.

Schuldiner

Itskovitz-Eldor

Benvenisty

(2003). Selective ablation of human embryonic stem cells expressing a “suicide” gene. Stem Cells 21:257-265.

21.

Sonoyama

Liu

Fang

Yamaza

Seo

Zhang

. (2006). Mesenchymal stem cell-mediated functional tooth regeneration in swine. PLoS One 1:e79.

22.

Takahashi

Yamanaka

(2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126:663-676.

23.

Tamaki

Nakahara

Ishikawa

Sato

(2013). In vitro analysis of mesenchymal stem cells derived from human teeth and bone marrow. Odontology 101:121-132.

24.

Tang

Xie

Pan

Wang

(2006). Mesenchymal stem cells participate in angiogenesis and improve heart function in rat model of myocardial ischemia with reperfusion. Eur J Cardiothorac Surg 30:353-361.

25.

Ueda

Fujisawa

Ono

Hara

Pham

Nakajima

. (2014). A short-term treatment with tumor necrosis factor-alpha enhances stem cell phenotype of human dental pulp cells. Stem Cell Res Ther 5:31.

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Mesenchymal Stem/Progenitor Cell Isolation from Tooth Extraction Sockets

Abstract

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