The acquired enamel pellicle (AEP) is a thin acellular film that forms on tooth surfaces upon exposure to the oral environment. It consists predominantly of salivary proteins, but also includes non-salivary-derived proteins, carbohydrates, and lipids. Since it is the interface between teeth and the oral environment, the AEP plays a key role in the maintenance of oral health by regulating processes including lubrication, demineralization, and remineralization and shaping the composition of early microbial flora adhering to tooth surfaces. Knowledge of the 3D structure of the AEP and how that correlates with its protective functions may provide insight into several oral pathological states, including caries, erosion, and periodontal disease. This review intends to update readers about the latest discoveries related to the formation, ultrastructure, composition, and functions of the AEP, as well as the future of pellicle research, with particular emphasis on the emerging role of proteomic and microscopy techniques in oral diagnosis and therapeutics.
AmaechiBTHighamSMEdgarWMMilosevicA (1999). Thickness of acquired salivary pellicle as a determinant of the sites of dental erosion. J Dent Res78:1821-1828.
2.
BaekJHKrasievaTTangSAhnYKimCSVuD. (2009). Optical approach to the salivary pellicle. J Biomed Opt14:044001.
3.
BergICRutlandMWArnebrantT (2003). Lubricating properties of the initial salivary pellicle—an AFM study. Biofouling19:365-369.
CardenasMElofssonULindhL (2007). Salivary mucin MUC5B could be an important component of in vitro pellicles of human saliva: an in situ ellipsometry and atomic force microscopy study. Biomacromolecules8:1149-1156.
6.
CarlénABorjessonACNikdelKOlssonJ (1998). Composition of pellicles formed in vivo on tooth surfaces in different parts of the dentition, and in vitro on hydroxyapatite. Caries Res32:447-455.
7.
CheaibZLussiA (2011). Impact of acquired enamel pellicle modification on initial dental erosion. Caries Res45:107-112.
8.
DeimlingDHannigCHoth-HannigWSchmitzPSchulte-MontingJHannigM (2007). Non-destructive visualisation of protective proteins in the in situ pellicle. Clin Oral Investig11:211-216.
9.
FeatherstoneJDBehrmanJMBellJE (1993). Effect of whole saliva components on enamel demineralization in vitro. Crit Rev Oral Biol Med4:357-362.
10.
FinkeMParkerDMJandtKD (2002). Influence of soft drinks on the thickness and morphology of in situ acquired pellicle layer on enamel. J Colloid Interface Sci251:263-270.
11.
GibbonsRJHayDIChildsWCDavisG (1990). Role of cryptic receptors (cryptitopes) in bacterial adhesion to oral surfaces. Arch Oral Biol35(Suppl):107S-114S.
12.
GregoireSXiaoJSilvaBBGonzalezIAgidiPSKleinMI. (2011). Role of glucosyltransferase B in interactions of Candida albicans with Streptococcus mutans and with an experimental pellicle on hydroxyapatite surfaces. Appl Environ Microbiol77:6357-6367.
13.
Hahn BergICLindhLArnebrantT (2004). Intraoral lubrication of PRP-1, statherin and mucin as studied by AFM. Biofouling20:65-70.
14.
HannigCHannigM (2009). The oral cavity—a key system to understand substratum-dependent bioadhesion on solid surfaces in man. Clin Oral Investig13:123-139.
15.
HannigCHannigMAttinT (2005). Enzymes in the acquired enamel pellicle. Eur J Oral Sci113:2-13.
16.
HannigCRuggeriAAl-KhayerBSchmitzPSpitzmullerBDeimlingD. (2008a). Electron microscopic detection and activity of glucosyltransferase B, C, and D in the in situ formed pellicle. Arch Oral Biol53:1003-1010.
17.
HannigCSpitzmullerBAl-AhmadAHannigM (2008b). Effects of Cistus-tea on bacterial colonization and enzyme activities of the in situ pellicle. J Dent36:540-545.
18.
HannigCSpitzmüllerBHannigM (2009a). Transaminases in the acquired pellicle. Arch Oral Biol54:445-448.
19.
HannigCBerndtDHoth-HannigWHannigM (2009b). The effect of acidic beverages on the ultrastructure of the acquired pellicle—an in situ study. Arch Oral Biol54:518-526.
20.
HannigCSpitzmüllerBHannigM (2009c). Characterisation of lysozyme activity in the in situ pellicle using a fluorimetric assay. Clin Oral Investig13:15-21.
21.
HannigCSpiesBSpitzmullerBHannigM (2010a). Efficacy of enzymatic mouth rinses for immobilisation of protective enzymes in the in situ pellicle. Arch Oral Biol55:1-6.
22.
HannigCSpitzmullerBLuxHCAltenburgerMAl-AhmadAHannigM (2010b). Efficacy of enzymatic toothpastes for immobilisation of protective enzymes in the in situ pellicle. Arch Oral Biol55:463-469.
23.
HannigM (1999). Ultrastructural investigation of pellicle morphogenesis at two different intraoral sites during a 24-h period. Clin Oral Investig3:88-95.
24.
HannigMBalzM (2001). Protective properties of salivary pellicles from two different intraoral sites on enamel erosion. Caries Res35:142-148.
25.
HannigMJoinerA (2006). The structure, function and properties of the acquired pellicle. Monogr Oral Sci19:29-64.
26.
HannigMHessNJHoth-HannigWDe VreseM (2003). Influence of salivary pellicle formation time on enamel demineralization—an in situ pilot study. Clin Oral Investig7:158-161.
27.
HannigMDobertAStiglerRMullerUProkhorovaSA (2004). Initial salivary pellicle formation on solid substrates studied by AFM. J Nanosci Nanotechnol4:532-538.
28.
HaraATZeroDT (2010). The caries environment: saliva, pellicle, diet, and hard tissue ultrastructure. Dent Clin North Am54:455-467.
29.
HaraATAndoMGonzalez-CabezasCCuryJASerraMCZeroDT (2006). Protective effect of the dental pellicle against erosive challenges in situ. J Dent Res85:612-616.
30.
HaukiojaALoimarantaVTenovuoJ (2008). Probiotic bacteria affect the composition of salivary pellicle and streptococcal adhesion in vitro. Oral Microbiol Immunol23:336-343.
31.
HayDI (1973). The interaction of human parotid salivary proteins with hydroxyapatite. Arch Oral Biol18:1517-1529.
32.
HayDIMorenoEC (1979). Differential adsorption and chemical affinities of proteins for apatitic surfaces. J Dent Res58(Spec Iss B):930-942.
33.
HelmerhorstEJAlaglASSiqueiraWLOppenheimFG (2006). Oral fluid proteolytic effects on histatin 5 structure and function. Arch Oral Biol51:1061-1070.
34.
IontchevaIOppenheimFGTroxlerRF (1997). Human salivary mucin MG1 selectively forms heterotypic complexes with amylase, proline-rich proteins, statherin, and histatins. J Dent Res76:734-743.
35.
JensenJLLamkinMSOppenheimFG (1992). Adsorption of human salivary proteins to hydroxyapatite: a comparison between whole saliva and glandular salivary secretions. J Dent Res71:1569-1576.
36.
JoinerAMullerDElofssonUMArnebrantT (2004). Ellipsometry analysis of the in vitro adsorption of tea polyphenols onto salivary pellicles. Eur J Oral Sci112:510-515.
37.
JoinerASchwarzAPhilpottsCJCoxTFHuberKHannigM (2008). The protective nature of pellicle towards toothpaste abrasion on enamel and dentine. J Dent36:360-368.
LamkinMSArancilloAAOppenheimFG (1996). Temporal and compositional characteristics of salivary protein adsorption to hydroxyapatite. J Dent Res75:803-808.
40.
LamkinMSMigliariDYaoYTroxlerRFOppenheimFG (2001). New in vitro model for the acquired enamel pellicle: pellicles formed from whole saliva show inter-subject consistency in protein composition and proteolytic fragmentation patterns. J Dent Res80:385-388.
41.
LendenmannUGroganJOppenheimFG (2000). Saliva and dental pellicle—a review. Adv Dent Res14:22-28.
42.
LiJHelmerhorstEJYaoYNunnMETroxlerRFOppenheimFG (2004). Statherin is an in vivo pellicle constituent: identification and immuno-quantification. Arch Oral Biol49:379-385.
43.
LongJRDindotJLZebroskiHKiihneSClarkRHCampbellAA. (1998). A peptide that inhibits hydroxyapatite growth is in an extended conformation on the crystal surface. Proc Natl Acad Sci USA95:12083-12087.
44.
MandelIDBennickA (1983). Quantitation of human salivary acidic proline-rich proteins in oral diseases. J Dent Res62:943-945.
45.
McDonaldEEGoldbergHATabbaraNMendesFMSiqueiraWL (2011). Histatin 1 resists proteolytic degradation when adsorbed to hydroxyapatite. J Dent Res90:268-272.
46.
NomuraYTamakiYTanakaTArakawaHTsurumotoAKirimuraK. (2006). Screening of periodontitis with salivary enzyme tests. J Oral Sci48:177-183.
47.
NyvadBFejerskovO (1987). Transmission electron microscopy of early microbial colonization of human enamel and root surfaces in vivo. Scand J Dent Res95:297-307.
48.
OudhoffMJBolscherJGNazmiKKalayHvan ’t HofWAmerongenAV. (2008). Histatins are the major wound-closure stimulating factors in human saliva as identified in a cell culture assay. FASEB J22:3805-3812.
49.
RajPAJohnssonMLevineMJNancollasGH (1992). Salivary statherin. Dependence on sequence, charge, hydrogen bonding potency, and helical conformation for adsorption to hydroxyapatite and inhibition of mineralization. J Biol Chem267:5968-5976.
50.
RichardsonCFJohnssonMRajPALevineMJNancollasGH (1993). The influence of histatin-5 fragments on the mineralization of hydroxyapatite. Arch Oral Biol38:997-1002.
51.
RudigerSGCarlénAMeurmanJHKariKOlssonJ (2002). Dental biofilms at healthy and inflamed gingival margins. J Clin Periodontol29:524-530.
52.
SchüpbachPOppenheimFGLendenmannULamkinMSYaoYGuggenheimB (2001). Electron-microscopic demonstration of proline-rich proteins, statherin, and histatins in acquired enamel pellicles in vitro. Eur J Oral Sci109:60-68.
53.
SiqueiraWLDawesC (2011). The salivary proteome: challenges and perspectives. Proteomics Clin Appl5:575-579.
54.
SiqueiraWLOppenheimFG (2009). Small molecular weight proteins/peptides present in the in vivo formed human acquired enamel pellicle. Arch Oral Biol54:437-444.
55.
SiqueiraWLHelmerhorstEJZhangWSalihEOppenheimFG (2007a). Acquired enamel pellicle and its potential role in oral diagnostics. Ann NY Acad Sci1098:504-509.
56.
SiqueiraWLZhangWHelmerhorstEJGygiSPOppenheimFG (2007b). Identification of protein components in in vivo human acquired enamel pellicle using LC-ESI-MS/MS. J Proteome Res6:2152-2160.
57.
SiqueiraWLMargolisHCHelmerhorstEJMendesFMOppenheimFG (2010). Evidence of intact histatins in the in vivo acquired enamel pellicle. J Dent Res89:626-630.
58.
SiqueiraWLBakkalMXiaoYSuttonJNMendesFM (2012). Quantitative proteomics analysis of the effect of fluoride on the acquired enamel pellicle. Plos ONE7:e42204.
59.
Sonju ClasenABHannigMSkjorlandKSonjuT (1997). Analytical and ultrastructural studies of pellicle on primary teeth. Acta Odontol Scand55:339-343.
60.
TabaMKinneyJKimASGiannobileWV (2005). Diagnostic biomarkers for oral and periodontal diseases. Dent Clin North Am49:551-571, vi.
61.
TabakLA (1995). In defense of the oral cavity: structure, biosynthesis, and function of salivary mucins. Annu Rev Physiol57:547-564.
62.
TabakLALevineMJMandelIDEllisonSA (1982). Role of salivary mucins in the protection of the oral cavity. J Oral Pathol11:1-17.
63.
TamakiNTadaTMoritaMWatanabeT (2002). Comparison of inhibitory activity on calcium phosphate precipitation by acidic proline-rich proteins, statherin, and histatin-1. Calcif Tissue Int71:59-62.
64.
TeixeiraEHNapimogaMHCarneiroVAde OliveiraTMCunhaRMHavtA. (2006). In vitro inhibition of streptococci binding to enamel acquired pellicle by plant lectins. J Appl Microbiol101:111-116.
65.
Vacca SmithAMBowenWH (2000). In situ studies of pellicle formation on hydroxyapatite discs. Arch Oral Biol45:277-291.
66.
van der MeiHCWhiteDJKamminga-RaskerHJKnightJBaigAASmitJ. (2002). Influence of dentifrices and dietary components in saliva on wettability of pellicle-coated enamelin vitro and in vivo. Eur J Oral Sci110:434-438.
VitorinoRLoboMJDuarteJRFerrer-CorreiaAJDominguesPMAmadoFM (2005). The role of salivary peptides in dental caries. Biomed Chromatogr19:214-222.
69.
VitorinoRde Morais GuedesSFerreiraRLoboMJDuarteJFerrer-CorreiaAJ. (2006). Two-dimensional electrophoresis study of in vitro pellicle formation and dental caries susceptibility. Eur J Oral Sci114:147-153.
70.
VitorinoRCalheiros-LoboMJWilliamsJFerrer-CorreiaAJTomerKBDuarteJA. (2007). Peptidomic analysis of human acquired enamel pellicle. Biomed Chromatogr21:1107-1117.
71.
VukosavljevicDCustodioWSiqueiraWL (2011). Salivary proteins as predictors and controls for oral health. J Cell Commun Signal5:271-275.
72.
WhittakerCJKlierCMKolenbranderPE (1996). Mechanisms of adhesion by oral bacteria. Annu Rev Microbiol50:513-552.
73.
XieHGibbonsRJHayDI (1991). Adhesive properties of strains of Fusobacterium nucleatum of the subspecies nucleatum, vincentii and polymorphum. Oral Microbiol Immunol6:257-263.
74.
YaoYLamkinMSOppenheimFG (2000). Pellicle precursor protein crosslinking characterization of an adduct between acidic proline-rich protein (PRP-1) and statherin generated by transglutaminase. J Dent Res79:930-938.
75.
YaoYBergEACostelloCETroxlerRFOppenheimFG (2003). Identification of protein components in human acquired enamel pellicle and whole saliva using novel proteomics approaches. J Biol Chem278:5300-5308.
76.
YoungARykkeMRöllaG (1999). Quantitative and qualitative analyses of human salivary micelle-like globules. Acta Odontol Scand57:105-110.
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