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Abstract

Light irradiation activates a range of cellular processes in a variety of cell types, including stem cells, and can promote tissue repair. This study investigated the effects of light-emitting diode (LED) exposure on dental pulp cells (DPCs). Dose response analysis at 20-second intervals up to 120 seconds demonstrated that a LED array emitting 653-nm red light stimulated significantly increased cell growth at 3 and 7 days post-irradiation with 40 (149 mJ/cm²) and 60 (224 mJ/cm²) seconds of radiant exposure. Double-dosing cells at days 1 and 4 of a 7-day culture period with 60-second (224 mJ/cm²) LED exposure significantly increased cell growth compared with a single dosing regime. BrdU analysis demonstrated significantly increased proliferation rates associated with significantly increased ATP, nitric oxide (NO), and mitochondrial metabolic activity. LED-stimulated NO levels were not reduced by inhibition of NO-synthase activity. Light exposure also rescued the inhibition of mitochondrial dysfunction and increased levels of in vitro mineralization compared with control. Media exchange experiments indicated that autocrine signaling was not likely responsible for red-light-induced DPC activity. In conclusion, data analysis indicated that 653-nm LED irradiation promoted DPC responses relevant to tissue repair, and this is likely mediated by increased mitochondrial activity.

Keywords

pulp biology biophotonics photobiomodulation dentin-pulp complex tooth tissue engineering stem cells

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References

Alghamdi

Kumar

Moussa

(2012). Low-level laser therapy: a useful technique for enhancing the proliferation of various cultured cells. Lasers Med Sci 27:237-249.

Ball

Castello

Poyton

(2011). Low intensity light stimulates nitrite-dependent nitric oxide synthesis but not oxygen consumption by cytochrome C oxidase: implications for phototherapy. J Photochem Photobiol B 102:182-191.

Barolet

(2008). Light-emitting diodes (LEDs) in dermatology. Semin Cutan Med Surg 27:227-238.

Chen

Arany

Huang

Tomkinson

Sharma

Kharkwal

. (2011). Low-level laser therapy activates NF-κB via generation of reactive oxygen species in mouse embryonic fibroblasts. PLoS One 6:e22453.

Choi

(2011). Protective effect of diazoxide against antimycin A-induced mitochondrial dysfunction in osteoblastic MC3T3-E1 cells. Toxicol In Vitro 25:1603-1608.

Corti

Chiarion-Sileni

Aversa

Ponzoni

D’Arcais

Pagnutti

. (2006). Treatment of chemotherapy induced oral mucositis with light-emitting diode. Photomed Laser Surg 24:207-213.

Danno

Horio

Imamura

(1992). Infrared radiation suppresses ultraviolet B-induced sunburn-cell formation. Arch Dermatol Res 284:92-94.

Desmet

Paz

Corry

Eells

Wong-Riley

Henry

. (2006). Clinical and experimental applications of NIR-LED photobiomodulation. Photomed Laser Surg 24:121-128.

Ferreira

Silveira

Genovese

de Araújo

Frigo

de Mesquita

. (2006). Effect of GaAIAs laser on reactional dentinogenesis induction in human teeth. Photomed Laser Surg 24:358-365.

10.

Gao

Xing

(2009). Molecular mechanisms of cell proliferation induced by low power laser irradiation. J Biomed Sci 16:4.

11.

Gartland

Orriss

Rumney

Bond

Arnett

Gallagher

(2012). Purinergic signalling in osteoblasts. Front Biosci 17:16-29.

12.

Giusti

JSM

Santos-Pinto

Pizzolitto

Kurachi

Bagnato

(2006). Effectiveness of Photogem^® activated by LED on the decontamination of artificial carious bovine dentin. Laser Phys 16:859-864.

13.

Gregory

Gunn

Peister

Prockop

(2004). An Alizarin red-based assay of mineralization by adherent cells in culture: comparison with cetylpyridinium chloride extraction. Anal Biochem 329:77-84.

14.

Huang

Chen

Carroll

Hamblin

(2009). Biphasic dose response in low level light therapy. Dose Response 7:358-383.

15.

Karu

(2010). Multiple roles of cytochrome C oxidase in mammalian cells under action of red and IR-A radiation. IUBMB Life 62:607-610.

16.

Lizarelli

Miguel

Villa

Filho

Pelino

Bagnato

(2007). Clinical effects of low-intensity laser vs light-emitting diode therapy on dentin hypersensitivity. J Oral Laser Appl 7:1-8.

17.

Matsui

Takeuchi

Tsujimoto

Matsushima

(2008). Effects of Smads and BMPs induced by Ga-Al-As laser irradiation on calcification ability of human dental pulp cells. J Oral Sci 50:75-81.

18.

Menezes

Coulomb

Lebreton

Dubertret

(1998). Non-coherent near infrared radiation protects normal human dermal fibroblasts from solar ultraviolet toxicity. J Invest Dermatol 111:629-633.

19.

Mima

Pavarina

Dovigo

Vergani

Costa

Kurachi

. (2010). Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 109:392-401.

20.

Mosmann

(1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55-63.

21.

Oliveira

Basso

Lins

Kurachi

Hebling

Bagnato

. (2011). In vitro effect of low-level laser on odontoblast-like cells. Lasers Phys Lett 8:155-163.

22.

Patel

Smith

Sloan

Smith

Cooper

(2009). Phenotype and behaviour of dental pulp cells during expansion culture. Arch Oral Biol 54:898-908.

23.

Pretel

Oliveira

Lizarelli

Ramalho

(2009). Evaluation of dental pulp repair using low level laser therapy (688 nm and 785 nm) morphologic study in capuchin monkeys. Lasers Phys Lett 6:149-158.

24.

Roelandts

(2005). A new light on Niels Finsen, a century after his Nobel Prize. Photodermatol Photoimmunol Photomed 21:115-117.

25.

Sacono

Costa

Bagnato

Abreu-e-Lima

(2008). Light-emitting diode therapy in chemotherapy-induced mucositis. Lasers Surg Med 40:625-633.

26.

Saracino

Mozzati

Martinasso

Pol

Canuto

Muzio

(2009). Superpulsed laser irradiation increases osteoblast activity via modulation of bone morphogenetic factors. Lasers Surg Med 41:298-304.

27.

Saura

Tarin

Zaragoza

(2010). Recent insights into the implication of nitric oxide in osteoblast differentiation and proliferation during bone development. Scientific World J 10:624-632.

28.

Toomarian

Fekrazad

Tadayon

Ramezani

Tunér

(2012). Stimulatory effect of low-level laser therapy on root development of rat molars: a preliminary study. Lasers Med Sci 27:537-542.

29.

Yasuhara

Suzawa

Miyamoto

Wang

Takami

Yamada

. (2007). Nitric oxide in pulp cell growth, differentiation, and mineralization. J Dent Res 86:163-168.

30.

Zheng

Wen

Austin

Chen

(2006). Exogenous nitric oxide stimulates cell proliferation via activation of a mitogen-activated protein kinase pathway in ovine fetoplacental artery endothelial cells. Biol Reprod 74:375-382.

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Effects of Red Light-emitting Diode Irradiation on Dental Pulp Cells

Abstract

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