The use of calcium-phosphate casein on hypomineralized molars (molar incisor hypomineralization, MIH) has been proposed but not clinically investigated. Qualitative and quantitative effects of supplementation with a calcium-phosphate casein product on MIH molars were monitored over a period of three years. Molar replicas, minimally invasive biopsies and their SEM microphotographs, plus ESEM/EDX semi-quantitative peaks of elements present in affected enamel were evaluated. Mineralization, morphology, and porosity appeared markedly improved, with calcium and phosphate levels reaching almost normal levels at three years’ follow-up. The hypothesis tested was rejected, since calcium-phosphate casein improved enamel morphology in vivo.
American Academy of Pediatric Dentistry Clinical Affairs Committee (2008). Guidelines on pediatric restorative dentistry. Reference manual. Pediatr Dent30(7 Suppl):163S-169S.
2.
ArnoldWGaenglerP (2007). Quantitative analysis of the calcium and phosphorus content of developing and permanent human teeth. Ann Anat189:183-190.
3.
AzarpazhoohALimebackH (2008). Clinical efficacy of casein derivatives: a systematic review of the literature. J Am Dent Assoc139:915-924.
4.
BertacciAChersoniSDavidsonCLPratiC (2007). In vivo enamel fluid movement. Eur J Oral Sci115:169-173.
5.
BertacciABaroniCMarchionniSGandolfiMGMontanariMPratiC (2008). Clinical management and SEM evaluation of MIH affected teeth. It J Operat Dent6:185-192.
6.
CochraneNJCaiFHuqNLBurrowMFReynoldsEC (2010). New approaches to enhanced remineralization of tooth enamel. J Dent Res DOI: 10.1177/0022034510381502.
7.
FarahRASwainMVDrummondBKCookRAtiehM (2010). Mineral density of hypomineralized enamel. J Dent38:50-58.
FDI Commission on Oral Health Research and Epidemiology (1992). A review of the development defects of enamel index: DDE Index. Int Dent J42:411-426.
10.
JälevikB (2010). Prevalence and diagnosis of molar-incisor-hypomineralization (MIH). A systematic review. Eur Arch Paediatr Dent10:59-64.
11.
JälevikBKlingbergGA (2002). Dental treatment, dental fear and behaviour management problems in children with severe enamel hypomineralization of their permanent first molars. Int J Paediatr Dent12:24-32.
12.
JälevikBNorénJG (2000). Enamel hypomineralization of first permanent molars: a morphological study and survey of possible aetiological factors. Int J Paediatr Dent10:278-289.
13.
JälevikBOdeliusHDietzWNorenJ (2001). Secondary ion mass spectrometry and x-ray microanalysis of hypomineralized enamel in human permanent molars. Arch Oral Biol46:239-247.
14.
KochMJGarcia-GodoyF (2000). The clinical performance of laboratory-fabricated crowns placed on first permanent molars with developmental defects. J Am Dent Assoc131:1285-1290.
15.
LygidakisNA (2010). Treatment modalities in children with teeth affected by molar-incisor enamel hypomineralization (MIH): a systematic review. Eur Arch Paediatr Dent2:65-74.
16.
LygidakisNAChaliasouASiounasG (2003). Evaluation of composite restorations in hypomineralized permanent molar: a four year clinical study. Eur J Paediatr Dent4:143-148.
17.
LygidakisNAWongFVierrouAMAlaluusuaSEspelidI (2010). Best clinical practice guidance for clinicians dealing with children presenting with molar incisors hypomineralization (MIH). An EAPD policy document. Eur Arch Paediatr Dent11:75-81.
18.
MangumJECrombieFAKilpatrickNMantonDJHubbardMJ (2010). Surface integrity governs the proteome of hypomineralized enamel. J Dent Res89:1160-1165.
19.
MejàreIBergmanEGrindefjordM (2005). Hypomineralized molars and incisors of unknown origin: treatment outcome at the age of 18 years. Int J Paediatr Dent15:20-28.
20.
ReynoldsEC (1997). Remineralization of enamel subsurface lesions by casein phosphopeptide-amorphous calcium-phosphate solutions. J Dent Res76:1587-1595.
21.
ReynoldsECCainCJWebberFL (1995). Anticariogenicity of calcium-phosphate complexes of tryptic casein phosphopeptides in the rat. J Dent Res74:1272-1279.
22.
ReynoldsECCainFShenPWalkerGD (2003). Retention in plaque and remineralization of enamel lesions by various forms of calcium in a mouthrinse of sugar-free chewing gum. J Dent Res82:206-211.
23.
SchulzeKABaloochMBaloochGMarshallGWMarshallSJ (2004). Micro-Raman spectroscopic investigation of dental calcified tissues. J Biomed Mater Res69:286-293.
24.
VenezieRDVadiakasGChristensenJRWrightJT (1994). Enamel pre-treatment with sodium hypochlorite to enhance bonding in hypocalcified amelogenesis imperfecta: a case report and SEM analysis. Pediatr Dent6:433-436.
25.
WeerheijmKLDuggalMMejàreIPapagiannoulisLKochGMartensLC. (2003). Judgement criteria for molar-incisors hypomineralization (MIH) in epidemiologic studies; a summary of the European meeting on MIH held in Athens 2003. Eur J Paediatr Dent4:110-113.
26.
WarshawskyH (1989). Organization of crystals of enamel. Anat Rec224:242-262.
27.
WilliamVMesserLBBurrowMF (2006). Molar incisor hypomineralization: review and recommendations for clinical management. Pediatr Dent28:224-232.
