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Abstract

This study examined the role of focal adhesion kinase (FAK) as a mechanoreceptor in human periodontal ligament (hPDL) cells. hPDL cells were obtained from premolars extracted for orthodontic purposes. Mechanical stress was applied in a compressive manner (2 g/cm²) for various time durations (0.5, 2, 6, 24, 48 hrs) with or without a knockdown treatment for FAK. Compressive stimulation increased the level of phosphorylated FAK and prostaglandin E₂ production. The FAK-knockdown cells showed significantly lower prostaglandin E₂ levels than the control cells. Furthermore, compressive stress up-regulated cyclo-oxygenase-2 mRNA, whereas there were no changes observed in the FAK-knockdown cells. These results suggest that FAK regulates the production of prostaglandin E₂ via the transcriptional regulation of COX-2 mRNA in compressive stimulated PDL cells. The FAK-integrin complex plays a role in mechanoreception and mechanotransduction in hPDL cells.

Keywords

focal adhesion kinase mechanical stress periodontal ligament

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References

Ando

Yamamoto

(2009). Vascular mechanobiology: endothelial cell responses to fluid shear stress. Circ J 73:1983-1992.

Aubin

Bonnelye

(2000). Osteoprotegerin and its ligand: a new paradigm for regulation of osteoclastogenesis and bone resorption. Osteoporos Int 11:905-913.

Bershadsky

Balaban

Geiger

(2003). Adhesion-dependent cell mechanosensitivity. Annu Rev Cell Dev Biol 19:677-695.

Bumann

Carvalho

Schwarzer

Yen

(1997). Collagen synthesis from human PDL cells following orthodontic tooth movement. Eur J Orthod 19:29-37.

Chiba

Mitani

(2004). Cytoskeletal changes and the system of regulation of alkaline phosphatase activity in human periodontal ligament cells induced by mechanical stress. Cell Biochem Funct 22:249-256.

Kanzaki

Chiba

Shimizu

Mitani

(2002). Periodontal ligament cells under mechanical stress induce osteoclastogenesis by receptor activator of nuclear factor kappaB ligand up-regulation via prostaglandin E₂ synthesis. J Bone Miner Res 17:210-220.

Katsumi

Orr

Tzima

Schwartz

(2004). Integrins in mechanotransduction. J Biol Chem 279:12001-12004.

Kim

Leucht

Luppen

Park

Beggs

Damsky

. (2007). Reconciling the roles of FAK in osteoblast differentiation, osteoclast remodeling, and bone regeneration. Bone 41:39-51.

Kim

Lee

Nahm

Kang

(2009). Effects of compressive stress on the expression of M-CSF, IL-1b, RANKL and OPG mRNA in periodontal ligament cells. Korean J Orthod 39:248-256.

10.

Knight

McGlashan

Garcia

Jensen

Poole

(2009). Articular chondrocytes express connexin 43 hemichannels and P2 receptors—a putative mechanoreceptor complex involving the primary cilium? J Anat 214:275-283.

11.

Liedert

Kaspar

Blakytny

Claes

Ignatius

(2006). Signal transduction pathways involved in mechanotransduction in bone cells. Biochem Biophys Res Commun 349:1-5.

12.

Mayahara

Kobayashi

Takimoto

Suzuki

Mitsui

Shimizu

(2007). Aging stimulates cyclooxygenase-2 expression and prostaglandin E₂ production in human periodontal ligament cells after the application of compressive force. J Periodontal Res 42:8-14.

13.

Molina

Kabsch

Alonso

Kohl

Komposch

Tomakidi

(2001). Topographic changes of focal adhesion components and modulation of p125FAK activation in stretched human periodontal ligament fibroblasts. J Dent Res 80:1984-1989.

14.

Papachristou

Papachroni

Basdra

Papavassiliou

(2009). Signaling networks and transcription factors regulating mechanotransduction in bone. Bioessays 31:794-804.

15.

Papachroni

Karatzas

Papavassiliou

Basdra

Papavassiliou

(2009). Mechanotransduction in osteoblast regulation and bone disease. Trends Mol Med 15:208-216.

16.

Raisz

Pilbeam

Fall

(1993). Prostaglandins: mechanisms of action and regulation of production in bone. Osteoporos Int 3(Suppl 1):136-140.

17.

Robinson

Chatterjee-Kishore

Yaworsky

Cullen

Zhao

. (2006). Wnt/beta-catenin signaling is a normal physiological response to mechanical loading in bone. J Biol Chem 281:31720-31728.

18.

Rubin

Jacobs

(2006). Molecular pathways mediating mechanical signaling in bone. Gene 367:1-16.

19.

van der Flier

Sonnenberg

(2001). Function and interactions of integrins. Cell Tissue Res 305:285-298.

20.

Wada

Maeda

Tanabe

Tsuda

Yano

Nakamuta

. (2001). Periodontal ligament cells secrete the factor that inhibits osteoclastic differentiation and function: the factor is osteoprotegerin/osteoclastogenesis inhibitory factor. J Periodontal Res 36:56-63.

21.

Yamamoto

Kita

Kimura

Oseko

Terauchi

Takahashi

. (2006). Mechanical stress induces expression of cytokines in human periodontal ligament cells. Oral Dis 12:171-175.

22.

Ziegler

Alonso

Steinberg

Woodnutt

Kohl

Mussig

. (2010). Mechano-transduction in periodontal ligament cells identifies activated states of MAP-kinases p42/44 and p38-stress kinase as a mechanism for MMP-13 expression. BMC Cell Biol 11:10.

FAK Pathway Regulates PGE 2 Production in Compressed Periodontal Ligament Cells

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