Sage Journals: Discover world-class research

Abstract

Important factors involved in odontogenesis in mouse dental papillae disappear between the pre- and post-natal stages of development. Therefore, we hypothesized that certain genes involved in odontogenesis in dental papillae were subject to pre-/post-natal down-regulation. Our goal was to identify, by microarray analysis, which genes were down-regulated. Dental papillae were isolated from embryonic 16-day-, 18-day- (E16, E18), and post-natal 3-day-old (P3) murine first mandibular molar germs and analyzed by microarray. The number of down-regulated genes was 2269 between E16 and E18, and 3130 between E18 and P3. Drastic down-regulation (fold change > 10.0) of Adamts4, Aldha1a2, and Lef1 was observed at both E16 and E18, and quantitative RT-PCR revealed a post-natal reduction in their expression (Adamts4, 1/3; Aldh1a2, 1/13; and Lef1, 1/37). These results suggest that down-regulation of these three genes is an important factor in normal odontogenesis in dental papillae.

Keywords

dental papilla dental pulp odontogenesis gene expression tooth morphogenesis

Get full access to this article

View all access options for this article.

References

Berkovitz

Maden

(1995). The distribution of cellular retinoic acid-binding protein I (CRABPI) and cellular retinol-binding protein I (CRBPI) during molar tooth development and eruption in the rat. Connect Tissue Res 32:191-199.

Bloch-Zupan

Decimo

Loriot

Mark

Ruch

(1994). Expression of nuclear retinoic acid receptors during mouse odontogenesis. Differentiation 57:195-203.

Brown

Knott

Halligan

Yarram

Mansell

Sandy

(2003). Microarray analysis of murine palatogenesis: temporal expression of genes during normal palate development. Dev Growth Differ 45:153-165.

Cho

Lee

Cai

Lee

Kim

Ohshima

. (2007). The primary enamel knot determines the position of the first buccal cusp in developing mice molars. Differentiation 75:441-451.

Hikake

Mori

Iseki

Hagino

Zhang

Takagi

. (2003). Comparison of expression patterns between CREB family transcription factor OASIS and proteoglycan core protein genes during murine tooth development. Anat Embryol (Berl) 206:373-380.

Hiratsuka

Kamino

Nagata

Takahashi

Asai

Ishikawa

. (2002). Microarray analysis of gene expression changes in aging in mouse submandibular gland. J Dent Res 81:679-682.

Hurmerinta

Thesleff

Saxén

(1980). In vitro inhibition of mouse odontoblast differentiation by vitamin A. Arch Oral Biol 25:385-393.

Jernvall

Thesleff

(2000). Reiterative signaling and patterning during mammalian tooth morphogenesis. Mech Dev 92:19-29.

Jones

Fabian

Kramer

(2008). The effect of retinoic acid on mouse mandibular molar development in vitro, using alkaline phosphatase as a molecular indicator of differentiation. S Afr Dent J 63:276, 278-280.

10.

Kollar

Baird

(1970). Tissue interactions in embryonic mouse tooth germs. I. Reorganization of the dental epithelium during tooth-germ reconstruction. J Embryol Exp Morphol 24:159-171.

11.

Kratochwil

Dull

Farinas

Galceran

Grosschedl

(1996). Lef1 expression is activated by BMP-4 and regulates inductive tissue interactions in tooth and hair development. Genes Dev 10:1382-1394.

12.

Kratochwil

Galceran

Tontsch

Roth

Grosschedl

(2002). FGF4, a direct target of LEF1 and Wnt signaling, can rescue the arrest of tooth organogenesis in Lef1(-/-) mice. Genes Dev 16:3173-3185.

13.

Kronmiller

Beeman

Kwiecien

Rollins

(1994). Effects of the intermediate retinoid metabolite retinal on the pattern of the dental lamina in vitro . Arch Oral Biol 39:839-845.

14.

Kuno

Kanada

Nakashima

Fujiki

Ichimura

Matsushima

(1997). Molecular cloning of a gene encoding a new type of metalloproteinase-disintegrin family protein with thrombospondin motifs as an inflammation associated gene. J Biol Chem 272:556-562.

15.

Mic

Haselbeck

Cuenca

Duester

(2002). Novel retinoic acid generating activities in the neural tube and heart identified by conditional rescue of Raldh2 null mutant mice. Development 129:2271-2282.

16.

Mina

Kollar

(1987). The induction of odontogenesis in non-dental mesenchyme combined with early murine mandibular arch epithelium. Arch Oral Biol 32:123-127.

17.

Nakamura

Fujii

Inoki

Sugimoto

Tanzawa

Matsuki

. (2000). Brevican is degraded by matrix metalloproteinases and aggrecanase-1 (ADAMTS4) at different sites. J Biol Chem 275:38885-38890.

18.

Niederreither

McCaffery

Drager

Chambon

Dolle

(1997). Restricted expression and retinoic acid-induced downregulation of the retinaldehyde dehydrogenase type 2 (RALDH-2) gene during mouse development. Mech Dev 62:67-78.

19.

Niederreither

Fraulob

Garnier

Chambon

Dolle

(2002). Differential expression of retinoic acid-synthesizing (RALDH) enzymes during fetal development and organ differentiation in the mouse. Mech Dev 110:165-171.

20.

Palmer

Lumsden

(1987). Development of periodontal ligament and alveolar bone in homografted recombinations of enamel organs and papillary, pulpal and follicular mesenchyme in the mouse. Arch Oral Biol 32:281-289.

21.

Sasaki

Ito

Han

Bringas

Jr Maeda

. (2005). LEF1 is a critical epithelial survival factor during tooth morphogenesis. Dev Biol 278:130-143.

22.

Sone

Nakamura

Maruya

Takahashi

Mizoguchi

Mayanagi

. (2005). Expression of Versican and ADAMTS during rat tooth eruption. J Mol Histol 36:281-288.

23.

Thesleff

(2003). Epithelial-mesenchymal signalling regulating tooth morphogenesis. J Cell Sci 116(Pt 9):1647-1648.

24.

Tortorella

Pratta

Liu

Abbaszade

Ross

Burn

. (2000). The thrombospondin motif of aggrecanase-1 (ADAMTS-4) is critical for aggrecan substrate recognition and cleavage. J Biol Chem 275:25791-25797.

25.

van Genderen

Okamura

Farinas

Quo

Parslow

Bruhn

. (1994). Development of several organs that require inductive epithelial-mesenchymal interactions is impaired in LEF-1-deficient mice. Genes Dev 8:2691-2703.

26.

Westling

Gottschall

Thompson

Cockburn

Perides

Zimmermann

. (2004). ADAMTS4 (aggrecanase-1) cleaves human brain versican V2 at Glu405-Gln406 to generate glial hyaluronate binding protein. Biochem J 377(Pt 3):787-795.

27.

Zhao

McCaffery

Ivins

Neve

Hogan

Chin

. (1996). Molecular identification of a major retinoic-acid-synthesizing enzyme, a retinaldehyde-specific dehydrogenase. Eur J Biochem 240:15-22.

Supplementary Material

Please find the following supplemental material available below.

For Open Access articles published under a Creative Commons License, all supplemental material carries the same license as the article it is associated with.

For non-Open Access articles published, all supplemental material carries a non-exclusive license, and permission requests for re-use of supplemental material or any part of supplemental material shall be sent directly to the copyright owner as specified in the copyright notice associated with the article.

0.00 MB

0.06 MB

Down-regulated Genes in Mouse Dental Papillae and Pulp

Abstract

Keywords

Get full access to this article

References

Supplementary Material