Sage Journals: Discover world-class research

Abstract

Local anesthetics have myotoxic effects and inhibit Ca-ATPase activity and Ca transport in skeletal muscles. Such effects have not been fully elucidated in masticatory muscles. We tested the hypothesis that local anesthetics increase myoplasmic calcium in masticatory muscles by inhibiting Ca-ATPase at a concentration similar to that of dental cartridges. The effects of lidocaine and bupivacaine on Ca-ATPase from rabbit masseter and medial pterygoid muscles were tested with radioisotopic and colorimetric methods. Bupivacaine had an action similar to that of lidocaine on Ca-ATPase activity, but less effect on calcium transport. The pre-exposure of the membranes to the anesthetics enhanced the Ca-ATPase activity in the absence of calcium ionophore, supporting their permeabilizing effect. The results demonstrate that amide-type anesthetics do not inhibit calcium binding, but do reduce calcium transport and enzyme phosphorylation by ATP, and suggest that the myoplasmic calcium increase induced by lidocaine and bupivacaine might promote masticatory muscle contraction and eventual rigidity.

Get full access to this article

View all access options for this article.

References

Alonso GL, Hecht JP (1990). Thermodynamics of Ca²⁺ transport through sarcoplasmic reticulum membranes during the transient state of simulated reactions. J Theoret Biol 147:161–176.

Baginski ES, Foa PP, Zak B (1967). Determination of phosphate: study of labile organic phosphate interference. Clin Chim Acta 15:155–158.

Benoit PW, Belt WD (1970). Destruction and regeneration of skeletal muscle after treatment with a local anaesthetic, bupivacaine (marcaine). J Anat 107(Pt 3):547–556.

Caravaca F, Soler F, Gomez-Fernandez JC, Fernandez-Belda F (1995). Drug action of ritodrine on the sarcoplasmic reticulum Ca²⁺-ATPase from skeletal muscle. Arch Biochem Biophys 318:97–104.

Champeil P, Guillain F, Vénien C, Gingold MP (1985). Interaction of magnesium and inorganic phosphate with calcium-deprived sarcoplasmic reticulum adenosinetriphosphatase as reflected by organic solvent induced perturbation. Biochemistry 24:69–81.

Clarke DM, Loo TW, Inesi G, MacLennan DH (1989). Location of high affinity Ca²⁺-binding sites within the predicted transmembrane domain of the sarcoplasmic reticulum Ca²⁺-ATPase. Nature 339:476–478.

Courtney KR (1980). Structure-activity relations for frequency-dependent sodium channel block in nerve by local anesthetics. J Pharmcacol Exp Ther 213:114–119.

Davidson GA, Berman MC (1988). Calcium dependence during single-cycle catalysis of the sarcoplasmic reticulum ATPase. J Biol Chem 263:11786–11791.

De Meis L (1981). The sarcoplasmic reticulum: transport and energy transduction. New York, USA: J. Wiley & Sons.

10.

Dolwick MF, Bush FM, Seibel HR, Burke GW (1977). Degenerative changes in masseter muscle following injection of lidocaine: a histo-chemical study. J Dent Res 56:1395–1402.

11.

Fabiato A, Fabiato F (1979). Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells. J Physiol (Paris) 75:463–505.

12.

Fraser RDB, Suzuki E (1974). The use of least squares in data analysis. In: Physical principles and techniques of protein chemistry: part C. Leach SJ, editor. NY, USA: Academic Press, pp. 301–355.

13.

Hecht JP, Nikonov JM, Alonso GL (1990). A BASIC program for the numerical solution of the transient kinetics of complex biochemical models. Comput Methods Programs Biomed 33:13–20.

14.

Lacapere J, Guillain F (1990). Reaction mechanism of Ca²⁺ ATPase of sarcoplasmic reticulum. Equilibrium and transient study of phosphorylation with Ca. ATP as substrate. J Biol Chem 265:8583–8589.

15.

Leo AJ (1991). Hydrophobic parameter: measurement and calculation. Methods Enzymol 202:544–591.

16.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951). Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275.

17.

Martinez-Azorín F, Teruel JA, Fernandez-Belda F, Gomez-Fernandez JC (1992). Effect of diethylstilbestrol and related compounds on the Ca(2+)-transporting ATPase of sarcoplasmic reticulum. J Biol Chem 267:11923–11929.

18.

Michelangeli F, Orlowski S, Champeil P, Grimes EA, East J, Lee AG (1990). Effects of phospholipids on binding of calcium to Ca²⁺-Mg²⁺-ATPase. Biochemistry 29:8307–8312.

19.

Okabe E, Kohono H, Kato Y, Odajima C, Ito H (1985). Characterization of the effect of pH on the excitation-contraction coupling system of canine masseter muscle. Jpn J Pharmacol 37:277–283.

20.

Sánchez GA, Takara D, Toma AF, Alonso GL (2004). Characteristics of the sarcoplasmic reticulum Ca²⁺-dependent ATPase from masticatory muscles. J Dent Res 83:557–561.

21.

Sokolove PM, Albuquerque EX, Kauffman FC, Spande TF, Daly JW (1986). Phenolic antioxidants: potent inhibitors of the (Ca²⁺- Mg ²⁺)-A TPase of sarcoplasmic reticulum. FEBS Lett 203: 121–126.

22.

Suko J, Winkler F, Scharinger B, Hellman G (1976). Aspects of the mechanism of action of local anesthetics on the sarcoplasmic reticulum of skeletal muscle. Biochim Biophys Acta 443:571–586.

23.

Takara D, Sánchez GA, Alonso GL (2000). Effect of carticaine on the sarcoplasmic reticulum Ca²⁺-dependent adenosine triphosphatase. Naunyn Schmiedebergs Arch Pharmacol 362:497–503.

24.

Takara D, Sánchez GA, Toma AF, Bonazzola P, Alonso GL (2005). Effect of on the sarcoplasmic reticulum Ca²⁺-d ependent adenosine triphosphatase. II: Cations dependence. Naunyn Schmiedebergs Arch Pharmacol 371:375–382.

25.

Widmer CG, Klugman D, English AW (1997). Anatomical partitioning and nerve branching patterns in the adult rabbit masseter. Acta Anat (Basel) 159:222–232.

26.

Wolosker H, Pacheco AG, De Meis L (1992). Local anesthetics induce fast Ca²⁺ efflux through a nonenergized state of the sarcoplasmic reticulum Ca²⁺-ATPase. J Biol Chem 267:5785–5789.

27.

Yagiela JA, Benoit PW, Buoncristiani RD, Peters MP, Fort NF (1981). Comparison of myotoxic effects of lidocaine with epinephrine in rats and humans. Anesth Analg 60:471–480.

28.

Zink W, Seif C, Bohl JR, Hacke N, Braun PM, Sinner B, et al. (2003). The acute myotoxic effects of bupivacaine and ropivacaine after continuous peripheral nerve blockades. Anesth Analg 97:1173–1179.

Supplementary Material

Please find the following supplemental material available below.

For Open Access articles published under a Creative Commons License, all supplemental material carries the same license as the article it is associated with.

For non-Open Access articles published, all supplemental material carries a non-exclusive license, and permission requests for re-use of supplemental material or any part of supplemental material shall be sent directly to the copyright owner as specified in the copyright notice associated with the article.

0.00 MB

0.63 MB

Local Anesthetics Inhibit Ca-ATPase in Masticatory Muscles

Abstract

Get full access to this article

References

Supplementary Material