Microbial Risk Markers for Childhood Caries in Pediatricians’ Offices

Abstract

Dental caries in pre-school children has significant public health and health disparity implications. To determine microbial risk markers for this infection, this study aimed to compare the microbiota of children with early childhood caries with that of caries-free children. Plaque samples from incisors, molars, and the tongue from 195 children attending pediatricians’ offices were assayed by 74 DNA probes and by PCR to Streptococcus mutans. Caries-associated factors included visible plaque, child age, race, and snacking habits. Species were detected more frequently from tooth than tongue samples. Lactobacillus gasseri (p < 0.01), Lactobacillus fermentum, Lactobacillus vaginalis, and S. mutans with Streptococcus sobrinus (all p < 0.05) were positively associated with caries. By multifactorial analysis, the probiotic Lactobacillus acidophilus was negatively associated with caries. Prevotella nigrescens was the only species (p < 0.05) significantly associated with caries by the ‘false discovery’ rate. Analysis of the data suggests that selected Lactobacillus species, in addition to mutans streptococci, are risk markers for early childhood caries.

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References

Aas JA, Griffen AL, Dardis SR, Lee AM, Olsen I, Dewhirst FE, et al. (2008). Bacteria of dental caries in primary and permanent teeth in children and young adults. J Clin Microbiol 46:1407–1417.

Alaluusua S, Malmivirta R (1994). Early plaque accumulation—a sign for caries risk in young children. Community Dent Oral Epidemiol 22(5 Pt 1):273–276.

Beltran-Aguilar ED, Barker LK, Canto MT, Dye BA, Gooch BF, Griffin SO, et al. (2005). Surveillance for dental caries, dental sealants, tooth retention, edentulism, and enamel fluorosis—United States, 1988–1994 and 1999–2002. MMWR Surveill Summ 54:1–43.

Benjamini Y, Hochberg Y (1995). Controlling the false discovery rate: a practical and powerful approach to multiple testing. J R Statist Soc B 57:289–300.

Caufield PW, Li Y, Dasanayake A, Saxena D (2007). Diversity of lactobacilli in the oral cavities of young women with dental caries. Caries Res 41:2–8.

Chhour KL, Nadkarni MA, Byun R, Martin FE, Jacques NA, Hunter N (2005). Molecular analysis of microbial diversity in advanced caries. J Clin Microbiol 43:843–849.

Corby PM, Lyons-Weiler J, Bretz WA, Hart TC, Aas JA, Boumenna T, et al. (2005). Microbial risk indicators of early childhood caries. J Clin Microbiol 43:5753–5759.

Dewhirst FE, Tamer MA, Ericson RE, Lau CN, Levanos VA, Boches SK, et al. (2000). The diversity of periodontal spirochetes by 16S rRNA analysis. Oral Microbiol Immunol 15:196–202.

Drury TF, Horowitz AM, Ismail AI, Maertens MP, Rozier RG, Selwitz RH (1999). Diagnosing and reporting early childhood caries for research purposes. A report of a workshop sponsored by the National Institute of Dental and Craniofacial Research, the Health Resources and Services Administration, and the Health Care Financing Administration. J Public Health Dent 59:192–197.

10.

Edelstein BL (2000). Access to dental care for Head Start enrollees. J Public Health Dent 60:221–229.

11.

Hellberg S, Sjöström M, Wold S (1986). The prediction of bradykinin potentiating potency of pentapeptides. An example of a peptide quantitative structure-activity relationship. Acta Chem Scand B 40:135–140.

12.

Kamma JJ, Diamanti-Kipioti A, Nakou M, Mitsis FJ (2000). Profile of subgingival microbiota in children with primary dentition. J Periodontal Res 35:33–41.

13.

Kashket S, Zhang J, Van Houte J (1996). Accumulation of fermentable sugars and metabolic acids in food particles that become entrapped on the dentition. J Dent Res 75:1885–1891.

14.

Könönen E, Asikainen S, Jousimies-Somer H (1992). The early colonization of Gram-negative anaerobic bacteria in edentulous infants. Oral Microbiol Immunol 7:28–31.

15.

Kressin RN, Nunn ME, Singh H, Orner MB, Pbert L, Hayes C, et al. (2009). Effects of a practice based intervention on pediatric providers’ counseling about children’s risk for dental decay. Medical Care 11:1121–1128.

16.

Kumar PS, Griffen AL, Moeschberger ML, Leys EJ (2005). Identification of candidate periodontal pathogens and beneficial species by quantitative 16S clonal analysis. J Clin Microbiol 43:3944–3955.

17.

Lee JY, Rozier RG, Norton EC, Kotch JB, Vann WF Jr (2004). The effects of the Women, Infants, and Children’s Supplemental Food Program on dentally related Medicaid expenditures. J Public Health Dent 64:76–81.

18.

Lewis CW, Grossman DC, Domoto PK, Deyo RA (2000). The role of the pediatrician in the oral health of children: a national survey. Pediatrics 106:E84.

19.

Marchant S, Brailsford SR, Twomey AC, Roberts GJ, Beighton D (2001). The predominant microflora of nursing caries lesions. Caries Res 35:397–406.

20.

Milgrom P, Riedy CA, Weinstein P, Tanner AC, Manibusan L, Bruss J (2000). Dental caries and its relationship to bacterial infection, hypoplasia, diet, and oral hygiene in 6- to 36-month-old children. Community Dent Oral Epidemiol 28:295–306.

21.

Mohebbi SZ, Virtanen JI, Vahid-Golpayegani M, Vehkalahti MM (2006). Early childhood caries and dental plaque among 1-3-year-olds in Tehran, Iran. J Indian Soc Pedod Prev Dent 24:177–181.

22.

Munson MA, Banerjee A, Watson TF, Wade WG (2004). Molecular analysis of the microflora associated with dental caries. J Clin Microbiol 42:3023–3029.

23.

Okada M, Soda Y, Hayashi F, Doi T, Suzuki J, Miura K, et al. (2005). Longitudinal study of dental caries incidence associated with Streptococcus mutans and Streptococcus sobrinus in pre-school children. J Med Microbiol 54(Pt 7):661–665.

24.

Paster BJ, Bartoszyk IM, Dewhirst FE (1998). Identification of oral streptococci using PCR-based, reverse-capture, checkerboard hybridization. Methods Cell Sci 20:223–231.

25.

Savino F, Pelle E, Palumeri E, Oggero R, Miniero R (2007). Lactobacillus reuteri (American Type Culture Collection Strain 55730) versus simethicone in the treatment of infantile colic: a prospective randomized study. Pediatrics 119:e124–e130.

26.

Seki M, Yamashita Y, Shibata Y, Torigoe H, Tsuda H, Maeno M (2006). Effect of mixed mutans streptococci colonization on caries development. Oral Microbiol Immunol 21:47–52.

27.

Shiboski CH, Gansky SA, Ramos-Gomez F, Ngo L, Isman R, Pollick HF (2003). The association of early childhood caries and race/ethnicity among California preschool children. J Public Health Dent 63:38–46; erratum in J Public Health Dent 63:264, 2003.

28.

Siegal MD, Marx ML, Cole SL (2005). Parent or caregiver, staff, and dentist perspectives on access to dental care issues for Head Start children in Ohio. Am J Public Health 95:1352–1359.

29.

Socransky SS, Haffajee AD, Smith C, Martin L, Haffajee JA, Uzel NG, et al. (2004). Use of checkerboard DNA-DNA hybridization to study complex microbial ecosystems. Oral Microbiol Immunol 9:352–362.

30.

Tanner AC, Milgrom PM, Kent R Jr, Mokeem SA, Page RC, Liao SI, et al. (2002a). Similarity of the oral microbiota of pre-school children with that of their caregivers in a population-based study. Oral Microbiol Immunol 17:379–387; erratum in Oral Microbiol Immunol 18:338, 2003.

31.

Tanner AC, Milgrom PM, Kent R Jr, Mokeem SA, Page RC, Riedy CA, et al. (2002b). The microbiota of young children from tooth and tongue samples. J Dent Res 81:53–57.

32.

Twetman S, Stecksén-Blicks C (2008). Probiotics and oral health effects in children. Int J Paediatr Dent 18:3–10.

33.

Van Houte J (1993). Microbiological predictors of caries risk. Adv Dent Res 7:87–96.

34.

Wennhall I, Matsson L, Schroder U, Twetman S (2002). Caries prevalence in 3-year-old children living in a low socio-economic multicultural urban area in southern Sweden. Swed Dent J 26:167–172.

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