Influence of Genetic Background on Fluoride Metabolism in Mice

Abstract

A/J and 129P3/J mouse strains have different susceptibilities to dental fluorosis, due to their genetic backgrounds. This study tested whether these differences are due to variations in water intake and/or F metabolism. A/J (susceptible to dental fluorosis) and 129P3/J mice (resistant) received drinking water containing 0, 10, or 50 ppm F. Weekly F intake, excretion and retention, and terminal plasma and femur F levels were determined. Dental fluorosis was evaluated clinically and by quantitative fluorescence (QF). Data were tested by two-way ANOVA. Although F intakes by the strains were similar, excretion by A/J mice was significantly higher due to greater urinary F excretion, which resulted in lower plasma and femur F levels. Compared with 129P3/J mice given 50 ppm F, significantly higher QF scores were recorded for A/J mice. In conclusion, these strains differ with respect to several features of F metabolism, and amelogenesis in the 129P3/J strain seems to be unaffected by high F exposure.

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References

Aoba T, Fejerskov O (2002). Dental fluorosis: chemistry and biology. Crit Rev Oral Biol Med 13:155–170.

Clark DC (1994). Trends in prevalence of dental fluorosis in North America. Community Dent Oral Epidemiol 22:148–152.

Everett ET, McHenry MA, Reynolds N, Eggertsson H, Sullivan J, Kantmann C, et al. (2002). Dental fluorosis: variability among different inbred mouse strains. J Dent Res 81:794–798.

Khan A, Packer NH (2006). Simple urinary sample preparation for proteomic analysis. J Proteome Res 5:2824–2838.

Marthaler TM (2004). Changes in dental caries 1953–2003. Caries Res 38: 173–181.

Mousny M, Banse X, Wise L, Everett ET, Hancock R, Vieth R, et al. (2006). The genetic influence on bone susceptibility to fluoride. Bone 39: 1283–1289.

Taves DR (1968). Determination of submicromolar concentrations of fluoride in biological samples. Talanta 15:1015–1023.

Thylstrup A, Fejerskov O (1978). Clinical appearance of dental fluorosis in permanent teeth in relation to histologic changes. Community Dent Oral Epidemiol 6:315–328.

Whitford GM (1991). Fluoride, calcium and phosphorus metabolism in the rat: comparison of ‘natural ingredient’ with semipurified diets. Arch Oral Biol 36:291–297.

10.

Whitford GM (1996). Metabolism and toxicity of fluoride. Monogr Oral Sci 16(Rev 2):1–153.

11.

Yoder KM, Mabelya L, Robison VA, Dunipace AJ, Brizendine EJ, Stookey GK (1998). Severe dental fluorosis in a Tanzanian population consuming water with negligible fluoride concentration. Community Dent Oral Epidemiol 26:382–393.

12.

Zegarelli EV (1944). Adamantoblastomas in the Slye stock of mice. Am J Pathol 20:23–87.