The incorporation of polymerizable cationic monomers has been attempted to generate dental resinous materials with antibacterial activity. This study tested the hypothesis that a dental adhesive containing a cationic monomer, methacryloxylethyl cetyl dimethyl ammonium chloride (DMAE-CB), would influence biofilm formation and gtf gene expression of Streptococcus mutans. The effect of the photo-polymerized DMAE-CB-incorporated adhesive on in vitro biofilm accumulation was investigated with spectrophotometry and scanning electron microscopy. The relative level of gtf gene expression by Streptococcus mutans in the biofilm was quantified by real-time reverse-transcription polymerase chain-reaction. The DMAE-CB-incorporated adhesive significantly decreased bio-film accumulation on its surface (P < 0.05), and suppressed the expression of gtfB and gtfC of Streptococcus mutans in the biofilm (P < 0.05). The results suggest that the cured DMAE-CB-incorporated adhesive may hamper biofilm accumulation via selective down-regulation of the expression of gtf genes in Streptococcus mutans.
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References
1.
Ajdic D, McShan WM, McLaughlin RE, Savic G, Chang J, Carson MB, et al. -2002-. Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen. Proc Natl Acad Sci USA99:14434–14439.
2.
Atac AS, Cehreli ZC, Sener B -2001-. Antibacterial activity of fifth-generation dentin bonding systems. J Endod27:730–733.
3.
Cury JA, Koo H -2007-. Extraction and purification of total RNA from Streptococcus mutans biofilms. Anal Biochem365:208–214.
4.
Ebi N, Imazato S, Noiri Y, Ebisu S -2001-. Inhibitory effects of resin composite containing bactericide-immobilized filler on plaque accumulation. Dent Mater17:485–491.
5.
Feuerstein O, Matalon S, Slutzky H, Weiss EI -2007-. Antibacterial properties of self-etching dental adhesive systems. J Am Dent Assoc138: 349–354.
6.
Fontana M, Gonzalez-Cabezas C -2000-. Secondary caries and restoration replacement: an unresolved problem. Compend Contin Educ Dent21:15–18, 21–14, 26.
7.
Fujiwara T, Hoshino T, Ooshima T, Hamada S -2002-. Differential and quantitative analyses of mRNA expression of glucosyltransferases from Streptococcus mutans MT8148. J Dent Res81:109–113.
8.
Gibbons RJ -1989-. Bacterial adhesion to oral tissues: a model for infectious diseases. J Dent Res68:750–760.
9.
Goodman SD, Gao Q -2000-. Characterization of the gtfB and gtfC promoters from Streptococcus mutans GS-5. Plasmid43:85–98.
10.
Hamada S, Slade HD -1980-. Biology, immunology, and cariogenicity of Streptococcus mutans.Microbiol Rev44:331–384.
11.
Imazato S -2003-. Antibacterial properties of resin composites and dentin bonding systems. Dent Mater19:449–457.
12.
Imazato S, Torii M, Tsuchitani Y, McCabe JF, Russell RR -1994-. Incorporation of bacterial inhibitor into resin composite. J Dent Res73:1437–1443.
13.
Imazato S, Ehara A, Torii M, Ebisu S -1998-. Antibacterial activity of dentine primer containing MDPB after curing. J Dent26:267–271.
14.
Imazato S, Ebi N, Takahashi Y, Kaneko T, Ebisu S, Russell RR -2003a-. Antibacterial activity of bactericide-immobilized filler for resin-based restoratives. Biomaterials24:3605–3609.
15.
Imazato S, Kinomoto Y, Tarumi H, Ebisu S, Tay FR -2003b-. Antibacterial activity and bonding characteristics of an adhesive resin containing antibacterial monomer MDPB. Dent Mater19:313–319.
16.
Imazato S, Kuramoto A, Takahashi Y, Ebisu S, Peters MC -2006-. In vitro antibacterial effects of the dentin primer of Clearfil Protect Bond. Dent Mater22:527–532.
17.
Imazato S, Tay FR, Kaneshiro AV, Takahashi Y, Ebisu S -2007-. An in vivo evaluation of bonding ability of comprehensive antibacterial adhesive system incorporating MDPB. Dent Mater23:170–176.
18.
Kuramitsu HK -1993-. Virulence factors of mutans streptococci: role of molecular genetics. Crit Rev Oral Biol Med4:159–176.
19.
Li Y, Burne RA -2001-. Regulation of the gtfBC and ftf genes of Streptococcus mutans in biofilms in response to pH and carbohydrate. Microbiology147-Pt 10-:2841–2848.
20.
Loesche WJ -1986-. Role of Streptococcus mutans in human dental decay. Microbiol Rev50:353–380.
21.
Mjör IA, Moorhead JE, Dahl JE -2000-. Reasons for replacement of restorations in permanent teeth in general dental practice. Int Dent J50:361–366.
22.
Montanaro L, Campoccia D, Rizzi S, Donati ME, Breschi L, Prati C, et al. -2004-. Evaluation of bacterial adhesion of Streptococcus mutans on dental restorative materials. Biomaterials25:4457–4463.
23.
Ooshima T, Matsumura M, Hoshino T, Kawabata S, Sobue S, Fujiwara T -2001-. Contributions of three glycosyltransferases to sucrose-dependent adherence of Streptococcus mutans.J Dent Res80:1672–1677.
24.
Shemesh M, Tam A, Feldman M, Steinberg D -2006-. Differential expression profiles of Streptococcus mutans ftf, gtf and vicR genes in the presence of dietary carbohydrates at early and late exponential growth phases. Carbohydr Res341:2090–2097.
25.
Shemesh M, Tam A, Steinberg D -2007-. Differential gene expression profiling of Streptococcus mutans cultured under biofilm and planktonic conditions. Microbiology153-Pt 5-:1307–1317.
26.
Sumitomo T, Nagamune H, Maeda T, Kourai H -2006-. Correlation between the bacterioclastic action of a bis-quaternary ammonium compound and outer membrane proteins. Biocontrol Sci11:115–124.
27.
Svanberg M, Mjör IA, Ørstavik D -1990-. Mutans streptococci in plaque from margins of amalgam, composite, and glass-ionomer restorations. J Dent Res69:861–864.
28.
Tovar JA, Duran C, Rodriguez A, Jaramillo L -2006-. Adhesion of salivary components to Streptococcus mutans peptides. Acta Odontol Latinoam19:53–58.
29.
Xiao YH, Chen JH, Fang M, Xing XD, Wang H, Wang YJ, et al. -2008-. Antibacterial effects of three experimental quaternary ammonium salt -QAS- monomers on bacteria associated with oral infections. J Oral Sci50:323–327.
30.
Yamashita Y, Bowen WH, Burne RA, Kuramitsu HK -1993-. Role of the Streptococcus mutans gtf genes in caries induction in the specific-pathogen-free rat model. Infect Immun61:3811–3817.
31.
Yoshimatsu T, Hiyama K -2007-. Mechanism of the action of didecyldime-thylammonium chloride -DDAC- against Escherichia coli and morphological changes of the cells. Biocontrol Sci12:93–99; erratum in Biocontrol Sci 12:159, 2007.
