Sage Journals: Discover world-class research

Abstract

In adhesive restorations, one major problem is hybrid layer degradation. At present, this deterioration is explained by the activation of the endogenous matrix metalloproteinases (MMPs) present in dentin due to the acidic property of adhesive systems. We hypothesized that self-etching adhesive should also stimulate the expression of MMPs in odontoblasts. In cultured tooth slices, we evaluated the changes in MMP-2 and proMMP-9 expression in the dentin-pulp complex after self-etching adhesive treatment on dentin cavities in immunochemistry and by zymography. The treatment resulted in increased MMP-2 expression in odontoblasts, as shown by immunohistochemistry. Zymography showed increased proMMP-9 and MMP-2 in dentin under self-etching treatment when pulp was present. These results showed that self-etching adhesive stimulates the secretion of MMPs from the dentin-pulp complex and, more precisely, by odontoblasts, suggesting that odontoblasts participate in hybrid layer degradation.

Get full access to this article

View all access options for this article.

References

Bartlett JD, Simmer JP (1999). Proteinases in developing dental enamel. Crit Rev Oral Biol Med 10:425–441.

Bourd-Boittin K, Fridman R, Fanchon S, Septier D, Golberg M, Menashi S (2005). Matrix metalloproteinase inhibition impairs the processing, formation and mineralization of dental tissues during mouse molar development. Exp Cell Res 304: 493–505.

Breschi L, Mazzoni A, Ruggeri A, Cadenaro M, Di Lenarda R, De Stefano Dorigo E (2008). Dental adhesion review: aging and stability of the bonded interface. Dent Mater 24:90–101.

Carrilho MR, Geraldeli S, Tay F, de Goes MF, Carvalho RM, Tjäderhane L et al. (2007). In vivo preservation of the hybrid layer by chlorhexidine. J Dent Res 86:529–533

Chaussain-Miller C, Fioretti F, Goldberg M, Menashi S (2006). The role of matrix metalloproteinases (MMPs) in human caries. J Dent Res 85:22–32.

De Munck J, Van Landuyt K, Peumans M, Poitevin A, Lambrechts P, Braem M, et al. (2005). A critical review of the durability of adhesion to tooth tissue: methods and results. J Dent Res 84:118–132.

Hebling J, Pashley DH, Tjäderhane L, Tay FR (2005). Chlorexhidine arrests subclinical degradation of dentin hybrid layers in vivo. J Dent Res 84:741–746.

Heikinheimo K, Salo T (1995). Expression of basement membrane type IV collagen and type IV collagenases (MMP-2 and MMP-9) in human fetal teeth. J Dent Res 74:1226–1234.

Koshiro K, Inoue S, Tanaka T, Koase K, Fujita M, Hashimoto M, et al. (2004). In vivo degradation of resin-dentin bonds produced by a self-etch vs. a total-etch adhesive system. Eur J Oral Sci 112: 368–375.

10.

Magloire H, Joffre A, Bleicher F (1996). An in vitro model of human dental pulp repair. J Dent Res 75:1971–1978.

11.

Magloire H, Lesage F, Couble ML, Lazdunski M, Bleicher F (2003). Expression and localization of TREK-1 K+ channels in human odontoblasts. J Dent Res 82:542–545.

12.

Martin-De Las Heras S, Valenzuela A, Overhall CM (2000). The matrix metalloproteinase gelatinase A in human dentine. Arch Oral Biol 45:757–765.

13.

Mazzoni A, Pashley DH, Nishitani Y, Breschi L, Mannello F, Tjäderhane L, et al. (2006). Reactivation of inactivated endogenous proteolytic activities in phosphoric acid-etched dentine by etch-and-rinse adhesives. Biomaterials 27:4470–4476.

14.

Mazzoni A, Mannello F, Tay FR, Tonti GAM, Papa S, Mazotti G, et al. (2007). Zymographic analysis and characterization of MMP-2 and -9 forms in human sound dentin. J Dent Res 86:436–440; erratum in J Dent Res 86:792, 2007.

15.

Melin M, Joffre Romeas A, Farges JC, Couble ML, Magloire H, Bleicher F (2000). Effects of TGFbeta1 on dental pulp cells in cultured human tooth slices. J Dent Res 79:1689–1696.

16.

Nagase H, Visse R, Murphy G (2006). Structure and function of metalloproteinases and TIMPs. Cardiovasc Res 69: 562–573.

17.

Nishitani Y, Yoshiyama M, Wadgaonkar B, Breschi L, Mannello F, Mazzoni A, et al. (2006). Activation of gelatinolytic/collagenolytic activity in dentin by self-etching adhesives. Eur J Oral Sci 114:160–166.

18.

Ntayi C, Labrousse AL, Debret R, Birembaut P, Bellon G, Antonicelli F, et al. (2004). Elastin derived peptides upregulate matrix metalloproteinase-2-mediated melanoma cell invasion through elastin-binding protein. J Invest Dermatol 122:256–265.

19.

Palosaari H, Ding Y, Larmas M, Sorsa T, Bartlett JD, Salo T, et al. (2002). Regulation and interactions of MT-1-MMP and MMP-20 in human odontoblasts and pulp tissue in vitro. J Dent Res 81:354–359.

20.

Palosaari H, Pennington CJ, Larmas M, Edwards DR, Tjäderhane L, Salo T (2003). Expression profile of matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs in mature human odontoblasts and pulp tissue. Eur J Oral Sci 111:117–127.

21.

Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM, et al. (2004). Collagen degradation by host-derived enzymes during aging. J Dent Res 83:216–221.

22.

Sulkala M, Wahlgren J, Larmas M, Sorsa T, Teronen O, Salo T, et al. (2001). The effects of MMP inhibitors on human salivary MMP activity and caries progression in rats. J Dent Res 80:1545–1549.

23.

Sulkala M, Pääkkönen V, Larmas M, Salo T, Tjäderhane (2004). Matrix metalloproteinase-13 (MMP-13, collagenase-3) is highly expressed in human tooth pulp. Connect Tissue Res 45:231–237.

24.

Sulkala M, Tervahartiala T, Sorsa T, Larmas M, Salo T, Tjäderhane L (2007). Matrix metalloproteinase-8 (MMP-8) is the major collagenase in human dentin. Arch Oral Biol 52:121–127.

25.

Tay FR, Pashley DH, Loushine RJ, Weller RN, Monticelli F, Osorio R (2006). Self-etching adhesives increase collagenolytic activity in radicular dentin. J Endod 32:862–868.

26.

Tjäderhane L, Salo T, Larjava H, Larmas M, Overall CM (1998a). A novel organ culture method to study the function of human odontoblasts in vitro: gelatinase expression by odontoblasts is differentially regulated by TGF-β1. J Dent Res 77:1486–1496.

27.

Tjäderhane L, Larjava H, Sorsa T, Uitto VJ, Larmas M, Salo T (1998b). The activation and function of host matrix metalloproteinases in dentin matrix breakdown in caries lesions. J Dent Res 77: 1622–1629.

28.

Tjäderhane L, Palosaari H, Sulkala M, Wahlgren J, Salo T (2002). The expression of matrix metalloproteinases (MMPs) in human odontoblasts. Proceedings of the International Conference on the Dentin/Pulp Complex, June 28–30, 2001, Kazusa, Japan. Ishikawa T, Takahashi K, Maeda T, Suda H, Shimono M, Inoue T, editors. Tokyo, Japan: Quintessence Publishing Co., pp. 45–51.

29.

Van Meerbeek B, De Munck J, Yoshida Y, Inoue S, Vargas M, Vijay P, et al. (2003). Buonocore memorial lecture. Adhesion to enamel and dentin: current status and future challenges. Oper Dent 28:215–235.

30.

Visse R, Nagase H (2003). Matrix metalloproteinases and tissue inhibitors of metalloproteinases: structure, function, and biochemistry. Circ Res 92:827–839.

Supplementary Material

Please find the following supplemental material available below.

For Open Access articles published under a Creative Commons License, all supplemental material carries the same license as the article it is associated with.

For non-Open Access articles published, all supplemental material carries a non-exclusive license, and permission requests for re-use of supplemental material or any part of supplemental material shall be sent directly to the copyright owner as specified in the copyright notice associated with the article.

0.00 MB

0.28 MB

Self-etching Increases Matrix Metalloproteinase Expression in the Dentin-Pulp Complex

Abstract

Get full access to this article

References

Supplementary Material