Periodontal Regeneration by FGF-2 (bFGF) in Primate Models

Abstract

We recently demonstrated that a topical application of basic fibroblast growth factor (FGF-2 ; bFGF) to alveolar bone defects in beagle dogs enhanced periodontal regeneration. The purpose of this study was further characterization of the biological effects of FGF-2 in non-human primates. Thirty-two inflamed furcation class II defects were surgically created in 4 male primates. The gelatinous carrier alone or the carrier containing 0.1 or 0.4% human recombinant FGF-2 was topically applied to the defects and compared with no treatment. Eight weeks after application, the periodontal regeneration in those defects was analyzed. In all FGF-2-treated sites, significant periodontal regeneration was dose-dependently observed in greater amounts than in the carrier-treated or non-treated sites. No instances of epithelial down-growth, ankylosis, or root resorption were observed in the FGF-2-treated sites. These results indicate that a topical application of FGF-2 can enhance considerable periodontal regeneration in surgically created furcation class II defects of non-human primates.

Keywords

basic fibroblast growth factor (FGF-2)wound healing periodontal regeneration tissue engineering,primate

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References

Gospodarowicz D. , Massoglia S. , Cheng J. , Fujii DK (1986). Effect of retina-derived basic and acidic fibroblast growth factor and lipoproteins on the proliferation of retina-derived capillary endothelial cells. Exp Eye Res 43:459-476.

Holt SC , Ebersole J. , Felton J. , Brunsvold M. , Kornman KS (1988). Implantation of Bacteroides gingivalis in nonhuman primates initiates progression of periodontitis. Science 239:55-57. Kawaguchi H. , Kurokawa T. , Hanada K. , Hiyama Y. , Tamura M. , Ogata E. , et al. (1994). Stimulation of fracture repair by recombinant human basic fibroblast growth factor in normal and streptozotocin-diabetic rats. Endocrinology 135:774-781.

Langer R. , Vacanti JP (1993). Tissue engineering. Science 260:920-926. Ledoux D. , Gannoun-Zaki L. , Barritault D. (1992). Interactions of FGFs with target cells. Prog Growth Factor Res 4:107-120.

Lynch SE , Williams RC , Polson AM , Howell TH , Reddy MS , Zappa UE , et al. (1989). A combination of platelet-derived and insulin-like growth factors enhances periodontal regeneration. J Clin Periodontol 16:545-548.

Mohammed S. , Pack Arc , Kardos TB (1998). The effect of

transforming growth factor beta one (TGF-β1) on wound healing, with or without barrier membranes, in a class II furcation defect in sheep. J Periodontal Res 33:335-344.

Murakami S. , Takayama S. , Ikezawa K. , Shimabukuro Y. , Kitamura M. , Nozaki T. , et al. (1999). Regeneration of periodontal tissues by basic fibroblast growth factor. J Periodontal Res 34:425-430. Nicosia RF , Bonanno E. , Smith M. , Yurchenco P. (1994). Modulation of angiogenesis in vitro by laminin-entactin complex. Dev Biol 164:197-206.

Park JB , Matsuura M. , Han KY , Norderyd 0 , Lin WL , Genco RJ , et al. (1995). Periodontal regeneration in class III furcation defects of beagle dogs using guided tissue regenerative therapy with platelet-derived growth factor. J Periodontol 66:462-477.

Robson MC , Phillips LG , Lawrence WT , Bishop JB , Youngerman JS , Hayward PG , et al. (1992). The safety and effect of topically applied recombinant basic fibroblast growth factor on the healing of chronic pressure sores. Ann Surg 216:401-408.

10.

Roesel JF , Nanney LB (1995). Assessment of differential cytokine effects on angiogenesis using an in vivo model of cutaneous wound repair. J Surg Res 58:449-459.

11.

Sigurdsson TJ , Lee MB , Kubota K. , Turek TJ , Wozney JM , Wikesjo Ume (1995). Periodontal repair in dogs: recombinant human bone morphogenetic protein-2 significantly enhances periodontal regeneration . J Periodontol 66:131-138.

12.

Szabo S. , Folkman J. , Vattay P. , Morales RE , Pinkus GS , Kato K. ( 1994). Accelerated healing of duodenal ulcers by oral administration of a mutein of basic fibroblast growth factor in rats. Gastroenterology 106:1106-1111.

13.

Tabata Y. , Yamada K. , Miyamoto S. , Nagata I. , Kikuchi H. , Aoyama I. , et al. (1998). Bone regeneration by basic fibroblast growth factor complexed with biodegradable hydrogels. Biomaterials 19 :807-815.

14.

Takayama S. , Murakami S. , Miki Y. , Ikezawa K. , Tasaka S. , Terashima A. , et al. (1997). Effects of basic fibroblast growth factor on human periodontal ligament cells. J Periodontal Res 32:667-675. Yanagisawa-Miwa A. , Uchida Y. , Nakamura F. , Tomaru T. , Kido H. , Kamijo T. , et al. (1992). Salvage of infarcted myocardium by angiogenic action of basic fibroblast growth factor. Science 257:1401-1403.