Cell-surface proteoglycans are involved in lymphocyte migration and activation. This study investigated the expression of syndecan-1, syndecan-4, and glypican in peripheral blood lymphocytes and by lymphocytes in variously inflamed periodontal tissues. Gingival specimens from healthy, gingivitis, or chronic periodontitis sites were stained by means of antibodies against B- and T-lymphocytes and also syndecan-1, syndecan-4, and glypican. Syndecan-1 expression by peripheral blood mononuclear cells (PBMC) from healthy, gingivitis, and chronic periodontitis subjects was assessed by flow cytometry. Syndecan-1 was expressed by B-cells/plasma cells but not T-cells in both gingivitis and chronic periodontitis lesions. Both B-cells/plasma cells and T-cells in gingivitis and chronic periodontitis expressed syndecan-4. Glypican was expressed only by macrophages. Stimulation of PBMC with mitogens and growth factors modulated syndecan-1 expression in both the T- and B-cells. Thus, cell-surface proteoglycan expression by lymphocytes in periodontal inflammation is cell-type-specific and may be modulated by inflammation.
Bernfield M., Kokenyesi R., Kato M., Hinkes MT, Spring J., Gallo RL, et al. (1992). Biology of the syndecans: a family of transmembrane heparan sulfate proteoglycans. Annu Rev Cell Biol8:365-393.
2.
Carey DJ (1997). Syndecans: multifunctional cell-surface co-receptors . Biochem J327:1-16.
3.
Couchman JR, Woods A. (1993). Structure and biology of pericellular proteoglycans . In: Cell surface and extracellular glycoconjugates, Roberts DD, Mecham RP , editors. San Diego, CA: Academic Press, Inc., pp. 33-82.
4.
David G., Bernfield M. (1998). The emerging roles of cell surface heparan sulfate proteoglycans. Matrix Biol17 :461-463.
5.
Dhodapkar MV, Sanderson RD (1999). Syndecan-1 (CD 138) in myeloma and lymphoid malignancies: a multifunctional regulator of cell behavior within the tumor microenvironment . Leuk Lymphoma34:35-43.
6.
Dhodapkar MV, Abe E., Theus A., Lacy M., Langford JK, Barlogie B., et al. (1998). Syndecan-1 is a multifunctional regulator of myeloma pathobiology: control of tumor cell survival, growth, and bone cell differentiation. Blood91:2679-2688.
7.
Gallagher JT (1989). The extended family of proteoglycans: social residents of the pericellular zone. Curr Opin Cell Biol1 :1201-1218.
8.
Hodgkin PD, Lee JH, Lyons AB (1996). B cell differentiation and isotype switching is related to division cycle number. J Exp Med184:277-281.
9.
Ishihara M., Fedarko NS, Conrad HE (1987). Involvement of phosphatidyl inositol and insulin in the coordinate regulation of proteoheparan sulfate metabolism and hepatocyte growth. J Biol Chem262:4708-4716.
10.
Jackson DG (1997). Human leucocyte heparan sulphate proteoglycans and their roles in inflammation. Biochem Soc Trans25:220-224.
11.
Kelly T., Borset M., Abe E., Gaddy-Kurten D., Sanderson RD (2000). Matrix metallo-proteinases in multiple myeloma . Leuk Lymphoma37:273-281.
12.
Kim CW, Goldberger OA, Gallo RL, Bernfield M. (1994). Members of the syndecan family of heparan sulfate proteoglycans are expressed in distinct cell-, tissue-, and development-specific patterns. Mol Biol Cell5:797-805.
13.
Lander AD, Stipp CS, Ivins JK (1996). The glypican family of heparan sulfate proteoglycans: major cell-surface proteoglycans of the developing nervous system. Perspect Dev Neurobiol3:347-358.
14.
Liu W., Litwack ED, Stanley MJ, Langford JK, Lander AD, Sanderson RD (1998). Heparan sulfate proteoglycans as adhesive and anti-invasive molecules. Syndecans and glypican have distinct functions. J Biol Chem273:22825-22832.
15.
McFall AJ, Rapraeger AC (1997). Identification of an adhesion site within the syndecan-4 extracellular protein domain. J Biol Chem272:12901-12904.
16.
Page RC, Offenbacher S., Schroeder HE, Seymour GJ, Kornman KS (1997). Advances in the pathogenesis of periodontitis: summary of developments, clinical implications and future directions. Periodontol200014:216-248.
17.
Rapraeger AC (1993). The coordinated regulation of heparan sulfate, syndecans and cell behavior. Curr Opin Cell Biol5:844-853.
18.
Ridley RC, Xiao H., Hata H., Woodliff J., Epstein J., Sanderson RD (1993). Expression of syndecan regulates human myeloma plasma cell adhesion to type I collagen. Blood81:767-774.
19.
Sanderson RD, Lalor P., Bernfield MB (1989). Lymphocytes express and lose syndecan at specific stages of differentiation. Cell Regul1:27-35.
20.
Selvan RS, Ihrcke NS, Platt JL (1996). Heparan sulfate in immune responses. Ann NYAcad Sci797:127-139.
21.
Seymour GJ (1991). Importance of the host response in the periodontium . J Clin Periodontol18:421-426.
22.
Smith KG, Hewitson TD, Nossal GJ, Tarlinton DM (1996). The phenotype and fate of the antibody-forming cells of the splenic foci. Eur J Immunol26:444-448.
23.
Stanley MJ, Liebersbach BF, Liu W., Anhalt DJ, Sanderson RD (1995). Heparan sulfate-mediated cell aggregation. Syndecans-1 and -4 mediate intercellular adhesion following their transfection into human B-lymphoid cells. J Biol Chem10(270):5077-5083.
24.
Stashenko P. (1982). Regulatory effect of monocytes on T cell proliferative responses to oral microbial antigens. Infect Immun38:938-947.
25.
Turner CA Jr, Mack DH, Davis MM (1994). Blimp-1, a novel zinc finger-containing protein that can drive the maturation of B-lymphocytes into immunoglobulin-secreting cells. Cell77 :297-306.
26.
Woods A., Oh ES, Couchman JR (1998). Syndecan proteoglycans and cell adhesion. Matrix Biol17:477-483.
27.
Worapamorn W., Li H., Haase HR, Pujic Z., Girjes AA, Bartold PM (2000). Cell surface proteoglycan expression by human periodontal cells. Connect Tissue Res41:57-68.
28.
Wrenshall LE, Carlson A., Cerra FB, Platt JL (1994). Modulation of cytolytic T cell responses by heparan sulfate. Transplantation57:1087-1094.
29.
Yamashita Y., Oritani K., Miyoshi EK, Wall R., Bernfield M., Kincade PW (1999). Syndecan-4 is expressed by B-lineage lymphocytes and can transmit a signal for formation of dendritic processes. J Immunol162:5940-5948.
30.
Zimmermann P., David G. (1999). The syndecans, tuners of transmembrane signaling . FASEB J13(Suppl):S91-S100.
