Gelatinase A (MMP-2) in Developing Tooth Tissues and Amelogenin Hydrolysis

Abstract

Matrix metalloproteinases (MMPs) are thought to play important roles during enamel and dentin biomineralization. Previously, membrane type-1 matrix metalloproteinase (MT1-MMP) was localized to the plasma membranes of ameloblasts and odontoblasts of the developing tooth. The best-characterized function of MT1-MMP is to initiate the activation of gelatinase A (MMP-2). Thus, we hypothesized that gelatinase A may also be expressed by developing tooth tissues. A full-length porcine gelatinase A mRNA was isolated by RT-PCR homology cloning of an enamel-organ-specific cDNA library. Northern blot and in situ hybridization analyses demonstrated gelatinase A expression in developing tooth tissues. Immunohistochemical analysis localized gelatinase A close to the plasma membrane of these tissues. Furthermore, recombinant gelatinase A was demonstrated to cleave recombinant amelogenin into several fragments of differing molecular masses. Thus, gelatinase A is expressed by developing tooth tissues along with its activator MT1-MMP and may, therefore, play an important role during tooth development.

Keywords

biomineralization MMP-2 amelogenin enamel dentin cDNA

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References

Aoba T. , Tanabe T. , Moreno EC (1987). Proteins in the enamel fluid of immature porcine teeth. J Dent Res 66:1721-1726.

Birkedal-Hansen H. , Moore WG , Bodden MK , Windsor LJ , Birkedal-Hansen B. , DeCarlo A. , et al. (1993). Matrix metalloproteinases: a review. Crit Rev Oral Biol Med 4:197-250.

Caron C. , Xue J. , Bartlett JD (1998). Expression and localization of membrane type 1 matrix metalloproteinase in tooth tissues. Matrix Biol 17:501-511.

Fincham AG , Moradian-Oldak J. (1996). Comparative mass spectrometric analyses of enamel matrix proteins from five species suggest a common pathway of post-secretory proteolytic processing. Connect Tissue Res 35:151-156.

Heikinheimo K. , Salo T. ( 1995). Expression of basement membrane type IV collagen and type IV collagenases (MMP-2 and MMP-9) in human fetal teeth. J Dent Res 74:1226-1234.

Hu CC , Bartlett JD , Zhang CH , Qian Q. , Ryu OH , Simmer JP (1996). Cloning, cDNA sequence, and alternative splicing of porcine amelogenin mRNAs. J Dent Res 75:1735-1741.

Hu JC , Zhang C. , Sun X. , Yang Y. , Cao X. , Ryu 0 , et al. (2000). Characterization of the mouse and human PRSS17 genes, their relationship to other serine proteases, and the expression of PRSS17 in developing mouse incisors. Gene 251:1-8.

Sahlberg C. , Reponen P. , Tryggvason K. , Thesleff I. (1992). Association between the expression of murine 72 kDa type IV collagenase by odontoblasts and basement membrane degradation during mouse tooth development. Arch Oral Biol 37:1021-1030.

Sahlberg C. , Reponen P. , Tryggvason K. , Thesleff I. (1999). Timp-1, -2 and -3 show coexpression with gelatinases A and B during mouse tooth morphogenesis. Eur J Oral Sci 107:121-130.

10.

Sato H. , Takino T. , Okada Y. , Cao J. , Shinagawa A. , Yamamoto E. , et al. (1994). A matrix metalloproteinase expressed on the surface of invasive tumour cells. Nature 370:61-65.

11.

Sato H. , Takino T. , Kinoshita T. , Imai K. , Okada Y. , Stetler-Stevenson WG , et al. (1996). Cell surface binding and activation of gelatinase A induced by expression of membrane-type-1-matrix metalloproteinase (MT1-MMP). FEBS Lett 385:238-240.

12.

Simmer JP , Lau EC , Hu CC , Aoba T. , Lacey M. , Nelson D. , et al. (1994). Isolation and characterization of a mouse amelogenin expressed in Escherichia coli. Calcif Tissue Int 54:312-319.

13.

Smith CE , Pompura JR , Borenstein S. , Fazel A. , Nanci A. (1989). Degradation and loss of matrix proteins from developing enamel. Anat Rec 224:292-316.

14.

Yamakoshi Y. , Tanabe T. , Fukae M. , Shimizu M. (1994). Porcine amelogenins. Calcif Tissue Int 54:69-75.