The Interactions of Human Neutrophils with the Constituents of an Experimental Dental Biofilm

Abstract

Despite the antibacterial properties of neutrophils, their ability to prevent colonization of the dental biofilm by pathogenic bacteria is limited. The present study examined the ability of human neutrophils to attach to an experimental dental biofilm and tested their antibacterial functions following adhesion. Neutrophil adhesion was greatest to hydroxyapatite (HA) in the absence of biofilm. Among the biofilms, glucosyltransferase or fructosyltransferase adsorbed onto saliva-coated HA showed the highest adhesion of cells. The adhesion of neutrophils was directly related to their initial concentration in the solution and to the duration of incubation. Plasma was found to reduce neutrophil attachment significantly, while stimulation of the cells had no effect. Stimulation of attached neutrophils induced superoxide secretion with levels significantly lower than that secreted by suspended cells. The presence of neutrophils on the biofilm reduced the number and the viability of Streptococcus mutans attached to the beads. The present findings suggest that neutrophils are able to attach to dental biofilms and that the attached neutrophils retained their antibacterial activity.

Keywords

neutrophils dental biofilm-experimental dental plaque host defense.

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References

Bauer WH (1946). The supporting tissue of tooth in acute secondary agranulocytosis (arsphenamin neutropenia). J Dent Res 25 :501-508.

Bignold LP (1986). Crawling-like movements of polymorphonuclear leukocytes in plasma are not a good index of their motility in microporous cellulose acetate membrane. Cell Biol Int Rep 10:535-543.

Cohen DW , Morris AL (1961). Periodontal manifestations of cyclic neutropenia . J Periodontol 32:159-168.

Dung TZ , Liu AH (1999). Molecular pathogenesis of root dentin caries. Oral Dis 5:92-99.

Erard JC , Miyasaki KT , Wolinsky LE (1989). Detachment of oral bacteria from saliva-coated hydroxyapatite by human polymorphonuclear leukocytes. J Periodontol 60:211-216.

Falck P. (1995). Characterization of human neutrophils adherent to organic polymers. Biomaterials 16:61-66.

Forrester JV , Lackie JM (1984). Adhesion of neutrophil leukocytes under conditions of flow. J Cell Sci 70:93-110.

Gold OG , Jordan HV , Van Houte J. (1973). A selective medium for Streptococcus mutans. Arch Oral Biol 18:1357-1364.

Kinoshita Y. , Miller ME , Wuest CJ (1989). Adherence assay of 51-chromium-labeled human polymorphonuclear leukocytes using glass beads. J Immunol Meth 124:29-33.

10.

Laurent F. , Benoliel AM , Capo C. , Bongrand P. (1991). Oxidative metabolism of polymorphonuclear leukocytes: modulation by adhesive stimuli. J Leukocyte Biol 49:217-226.

11.

Mishkin DJ , Akers JO , Darby CP (1976). Congenital neutropenia. Report of a case and a biorationale for dental management. Oral Surg Oral Med Oral Pathol 42:738-745.

12.

Miyasaki KT (1991). The neutrophil: mechanism of controlling periodontal bacteria. J Periodontol 62:761-774.

13.

Passo SA , Tsai CC , McArthur WP , Leifer C. , Taichman NS (1980). Interaction of inflammatory cells and oral microorganisms. IX. The bactericidal effects of human polymorphonuclear leukocytes on isolated plaque microorganisms. J Periodont Res 15:470-482.

14.

Pernu HE , Pajari UH , Lanning M. (1996). The importance of regular dental treatment in patients with cyclic neutropenia. Follow-up of 2 cases. J Periodontol 67:454-459.

15.

Schilling KM , Blitzer MH , Bowen WH (1989). Adherence of Streptococcus mutans to glucans formed in situ in salivary pellicles. J Dent Res 68(Spec Iss):1678-1680.

16.

Schroeder HE , Attstrom R. (1980). Pocket formation: an hypothesis. In: The borderline between caries and periodontal diseases. Lehner T , Cimasoni G , editors. London: Academic Press, pp. 99-123.

17.

Shapira L. , Borinski R. , Sela MN , Soskolne A. (1991). Superoxide formation and chemiluminescence of peripheral polymorphonuclear leukocytes in rapidly progressive periodontitis patients. J Clin Periodontol 18:44-48.

18.

Shapira L. , Takashiba S. , Kalmar JR , Van Dyke TE , Barak V. , Soskolne WA (1993). Rapid fluorometric quantification of monocyte attachment in tissue culture wells. J Immunol Meth 165:93-98.

19.

Shapira L. , Gordon B. , Warbington M. , Van Dyke TE (1994). Priming effect of Porphyromonas gingivalis lipopolysaccharide on superoxide production by neutrophils from healthy and rapidly progressive periodontitis subjects. J Periodontol 65:129-133.

20.

Steinberg D. , Beeman D. , Bowen WH (1992). The effect of delmopinol on glucosyltransferase adsorbed onto saliva coated hydroxyapatite. Arch Oral Biol 37:33-38.

21.

Steinberg D. , Kopec LK , Bowen WH (1993). Adhesion of actinomyces isolates to experimental pellicles. J Dent Res 72:1015-1020.

22.

Steinberg D. , Poran S. , Shapira L. (1999). The effect of extracellular polysaccharides from Streptococcus mutans on the bactericidal activity of human neutrophils. Arch Oral Biol 44:437-444.

23.

Tanigawa T. , Kotake Y. , Tanigawa M. , Reinke LA (1995). Mutual contact of adherent polymorphonuclear leukocytes inhibits their generation of superoxide. Free Radic Res 22:361-373.

24.

Venkitaraman AR , Vacca-Smith AM , Kopec LK , Bowen WH (1995). Characterization of glucosyltransferaseB, GtfC, and GtfD in solution and on the surface of hydroxyapatite. J Dent Res 74:1695-1701.

25.

Waldrop TC , Anderson DC , Hallmon WW , Schmalstieg FC , Jacob RL (1987). Periodontal manifestations of heritable Mac-1, LFA-1 deficiency syndrome. Clinical, histopathological and molecular characteristics . J Periodontol 58:400-416.