Enamelysin is a recently isolated member of the matrix metalloproteinase (MMP) family of extracellular matrix (ECM)-degrading enzymes. Here we describe the isolation and characterization of the mouse enamelysin cDNA. Expression of mouse enamelysin was detectable only in ameloblasts and odontoblasts of developing teeth. Characterization of mouse enamelysin demonstrated that it is highly conserved in both its sequence content and pattern of expression relative to the porcine, human, and bovine homologues previously described.
a 21 kDa and 25 kDa bovine enamel metalloproteinase. Eur J Oral Sci106:345-349.
2.
Fukae M., Tanabe T., Uchida T., Lee SK, Ryu OH, Murakami C., et al. (1998). Enamelysin (matrix metalloproteinase-20): localization in the developing tooth and effects of pH and calcium on amelogenin hydrolysis. J Dent Res77:1580-1588.
3.
Llano E., Pendas AM, Knauper V., Sorsa T., Salo T., Salido E., et al. (1997). Identification and structural and functional characterization of human enamelysin (MMP-20). Biochemistry36:15101-15108.
4.
Massova I., Kotra LP, Fridman R., Mobashery S. (1998). Matrix metalloproteinases: structures, evolution, and diversification. FASEB J12:1075-1095.
5.
Matrisian LM (1992). The matrix-degrading metalloproteinases. Bioessays14:455-463.
6.
Ryu OH, Fincham AG, Hu CC, Zhang C., Qian Q., Bartlett JD, et al. (1999). Characterization of recombinant pig enamelysin activity and cleavage of recombinant pig and mouse amelogenins. J Dent Res78:743-750.
7.
Sambrook J., Fritsch EF, Maniatis T. (1989). Molecular cloning-a laboratory manual. 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
8.
Simmer JP, Fincham AG (1995). Molecular mechanisms of dental enamel formation . Crit Rev Oral Biol Med6:84-108.
9.
Smith CE, Pompura JR, Borenstein S., Fazel A., Nanci A. (1989). Degradation and loss of matrix proteins from developing enamel. Anat Rec224:292-316.
10.
Snead ML, Bringas P. Jr, Bessem C., Slavkin HC (1984). De novo gene expression detected by amelogenin gene transcript analysis. Dev Biol104:255-258.
11.
Stetler-Stevenson WG, Aznavoorian S., Liotta LA (1993). Tumor cell interactions with the extracellular matrix during invasion and metastasis. Ann Rev Cell Biol9:541-573.
12.
Woessner JF Jr (1991). Matrix metalloproteinases and their inhibitors in connective tissue remodeling. FASEB J5:2145-2154.
13.
Woessner JF (1998). Role of matrix proteases in processing enamel proteins. Connect Tissue Res39:373-377.