Optical Scattering Power for Characterization of Mineral Loss

Abstract

Mineral loss in early caries cannot be measured without invasive procedures. To quantify mineral loss without sectioning the tooth, one must determine the optical scattering of the enamel. Using enamel white-spot lesions, we hypothesize that the optical scattering power (Sp) of the demineralized enamel would provide a quantitative estimate of mineral loss. Enamel slabs were demineralized to produce artificial white spots. The data were acquired by means of a Charge-Coupled Device (CCD) camera and image-processing software. For the purpose of comparison, mineral loss (ΔZ) of the demineralized samples was determined by the use of a microhardness approach after the samples were sectioned. The scattering power correlated well with AZ (r² = 0.82). In contrast, simple reflectance of the demineralized samples correlated poorly with AZ (r² = 0.22). The validity of using scattering power to measure demineralization has been confirmed by a three-dimensional Monte Carlo Simulation.

Keywords

optical scattering power enamel caries white spot lesion mineral loss.

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References

Arends J. , Christoffersen J. (1986). The nature of early caries lesions in enamel. J Dent Res 65:2-11.

Arends J. , ten Bosch JJ (1986). In vivo de- and remineralization of dental enamel . In: Factors Relating to Demineralisation and Remineralisation of the Teeth. Leach SA , editor. Oxford, England: IRL Press Limited, pp.1-11.

Brinkman J. , ten Bosch JJ , Borsboom PC (1988). Optical quantitation of natural caries in smooth surfaces of extracted teeth. Caries Res 22:257-262.

Featherstone JD , ten Cate JM , Shariati M. , Arends J. (1983). Comparison of artificial caries-like lesions by quantitative microradiography and microhardness profiles. Caries Res 17:385-391.

Fried D. , Glena RE , Featherstone Jdb , Seka W. ( 1995). Nature of light scattering in dental enamel and dentin at visible and near-infrared wavelengths. Appl Optics 34:1278-1285.

Kerr DA , Ash MM Jr, Millard HD , editors (1983). Oral diagnosis. 6th ed. St. Louis : C.V. Mosby.

Kortüm G. (1969). Reflectance spectroscopy. Principles, methods, applications. New York: Springer-Verlag .

0gaard B. , Rølla G. , Arends J. (1988). Orthodontic appliances and enamel demineralization. Part 1. Lesion development. Am J Orthod Dentofac Orthop 94:68-73.

Primrose J. , Geddes DA , Weetman DA (1989). Development of a screening test for the determination of the cariogenic potential of foods. Caries Res 23:165-167.

10.

Spitzer D. , ten Bosch JJ (1977). Luminescence quantum yields of sound and carious dental enamel. Calcif Tissue Res 24:249-251.

11.

Star WM , Marijnissen JP , van Gemert MJ (1988). Light dosimetry in optical phantoms and in tissues: I. Multiple flux and transport theory. Phys Med Biol 33:437-454.

12.

ten Bosch JJ , Coops JC (1995). Tooth color and reflectance as related to light scattering and enamel hardness. J Dent Res 74:374-380.

13.

ten Bosch JJ , van der Mei HC , Borsboom PC (1984). Optical monitor of in vitro caries: a comparison with chemical and microradiographic determination of mineral loss in early lesions. Caries Res 18:540-547.

14.

van de Hulst HC (1980). Multiple light scattering: tables, formulas, and application. Vol. 1. New York: Academic Press.

15.

Wang L. , Jacques SL , Zheng L. (1995). MCML-Monte Carlo modeling of light transport in multi-layered tissues. Comput Meth Programs Biomed 47:131-146.

16.

Wilson BC , Adam G. ( 1983). A Monte Carlo model for the absorption and flux distributions of light in tissue. Med Phy 10:824-830.

17.

Zijp JR , ten Bosch JJ , Groenhuis RA (1995). HeNe-laser light scattering by human dental enamel . J Dent Res 74:1891-1898.