Increased Creatine Kinase Brain Isoenzyme Concentration in Cerebrospinal Fluid with Meningitis

Abstract

CPK-BB (CK-BB) isoenzyme is an intracellular enzyme released in various neurologic conditions, including central nervous system (CNS) infections. Activity of CK-BB in cerebrospinal fluid (CSF) was determined in 80 children by electrophoresis and densitometry. The possible correlation between CNS infection and CK concentrations was assessed. Significantly elevated concentrations of CK activity (P <0.01) in the CSF were found in children with bacterial meningitis as compared with children with either aseptic meningitis or normal CSF findings. The data suggest the possibility of utilizing CSF CK activity to differentiate between bacterial and viral meningitis in situations where a routine CSF examination is inconclusive.

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References

1. Sacher RA , McPherson RA . Enzymes useful in clinical chemistry diagnosis: creatine kinase. In: Widmann's Clinical Interpretation of Laboratory Tests, 10th ed., Philadelphia: FA Davis Company; 1991.

2. Bakay RAE , Ward AA . Enzymatic changes in serum and cerebrospinal fluid in neurological injury. J Neurosurg. 1983;58:27-37.

3. Hans P , Albert A , Franssen C , et al. Improved outcome prediction based on CSF exterpolated creatine kinase BB isoenzyme activity and other risk factors in severe head injury. JNeurosurg. 1989;71:54-58.

4. Hans P , BornJD , ChapelleJP , et al. Creatine kinase isoenzymes in severe head injury.JNeurosurg. 1983;58:689-692.

5. Pfeiffer FE , Homburger HA , Yanagihara T . Creatine kinase BB isoenzyme in CSF in neurological diseases. Measurement by radioimmunoassay. Arch Neurol. 1983;40:169-172.

6. Worley G , Lipman B , Gewolb IH , et al. Creatine kinase brain isoenzyme: relationship of cerebrospinal fluid concentration to the neurologic condition of newborns and cellular localization in the human brain. Pediatrics. 1985;76:15-21.

7. De Praeter C , Vanhaesebrouck P , Govaert P , et al. Creatine kinase isoenzyme BB concentrations in the cerebrospinal fluid of newborns: relationship to short-term outcome. Pediatrics. 1991;88:1204-1210.

8. Chapelle JP , Heusghem C . Further heterogeneity demonstrated for serum creatine kinase isoenzyme MM. Clin Chem. 1980;26:457-462.

9. Thompson RJ , GrahamJG , McQueen INF , et al. Radioimmunoassay of brain type creatine kinase-BB isoenzyme in human tissues and in serum of patients with neurological disorders. Neurol Sci. 1980;47:241-251.

10.

10. Cho HW , Prilipko L , Meltzer HY , Keller L . Isoenzymes of creatine phosphokinase in white blood cells. Experientia. 1977;33:166-167.