Comparative studies of LFA‐1/ICAM‐1 interaction by micropipette and flow chamber techniques

Abstract

Interaction of lymphocyte function‐associated antigen‐1 (LFA‐1) with intercellular adhesive molecule‐1 (ICAM‐1) is important in a number of cellular events, including inflammation, adhesion, transendothelial migration. The aim of this work was to study comparatively the adhesive interaction between LFA‐1 and ICAM‐1 by a micropipette technique and a flow chamber method, and also to explore the effects of tumor necrosis factor (TNF‐α), phytohemagglutinin (PHA), and tetramethylpyrazine (TMP) on this interaction. The adhesion probability (Pa) between a lymphocyte cell line SKW‐3 expressing LFA‐1 and a red blood cell (RBC) coated with soluble ICAM‐1 was approached by the micropipette technique, while the flow chamber allowed to observe the firm adhesion of SKW‐3 on human umbilical vein endothelial cells (HUVECs). Experimental results show that PHA stimulation of lymphocytes resulted in significant increases in the adhesion probability (Pa) and in number of firmly adhered lymphocytes to HUVECs, but TMP treatment could significantly inhibit such increases.