The Liver: New Functional,Prognostic and Diagnostic Tests

Sherlock S . Disease of the Liver and Biliary System. Oxford: Blackwell Scientific Publications, 1987.

Johnson PJ . Role of the standard ‘liver function tests' in current clinical practice. Ann Clin Biochem 1989; 25: 463–71.

Galambos JT Wills CE . Relationship between 505 paired liver tests and biopsies in 242 obese patients. Gastroenterology 1978; 74: 1191–5.

Van Ness MM Diehl AM . Is liver biopsy useful in the evaluation of patients with chronically elevated liver enzymes? Ann Intern Med 1989; 111: 473–8.

Laker MF . Liver function tests. BMJ 1990; 301: 250–1.

Sallie R Tredger JM Williams R . Drugs and the liver. Part 1: Testing liver function. Biopharm Drug Dispos 1991; 12: 251–9.

Tang HS Hu OY . Assessment of liver function using a novel galactose single point method. Digestion 1992; 52: 222–31.

Bergstrom M Soderman C Eriksson LS . A simplified method to determine galactose elimination capacity in patients with liver disease. Scand J Clin Lab Invest 1993; 53: 667–70.

Tygstrup N . Determination of the hepatic elimination capacity (L_m) of galactose by single injection. Scand J Clin Invest 1966; 18: 118–25.

Reichen J Widmer T Cotting J . Accurate prediction of death by serial determination of galactose elimination capacity in primary biliary cirrhosis: A comparison with the Mayo model. Hepatology 1991; 14: 504–10.

Reichen J Bianchi L Frei PC Male PJ Lavanchy D Schmid M . Efficacy of steroid withdrawal and low-dose interferon treatment in chronic active hepatitis B. Results of a randomised multicentre trial. Swiss Association for the study of the liver. J Hepatol 1994; 20: 168–74.

Schneider JF Baker AL Haines NW Hatfield G Boyer JL . Aminopyrine N-demethylation: a prognostic test of liver function in patients with alcoholic liver disease. Gastroenterology 1980; 79: 1145–50.

Hepner GW Vesell ES . Assessment of aminopyrine metabolism in man by breath analysis after oral administration of ¹⁴C-aminopyrine. Effects of phenobarbital, disulfiram and portal cirrhosis. N Engl J Med 1974; 291: 1384–8.

Merkel C Bolognesis M Bellon S Bianco S Honisch B Lampe H Aminopyrine breath test in the prognostic evaluation of patients with cirrhosis. Gut 1992; 33: 836–42.

Caesar J Shaldon S Chiandussi L Guevara L Sherlock S . The use of indocyanine green in measurement of hepatic blood flow and as a test of hepatic function. Clin Sci 1961; 21: 43–57.

MacMathuna P Westaby D Williams R . Portal hypertension: Pathophysiology, diagnosis and treatment. In: Millward-Sadler GH Wright R Arthur MJP , eds. Wright's Liver and Biliary Disease, 3rd edn. London: WB Saunders, 1992: 1296–322.

Ikeda K Saitoh S Koida I Arase Y Tsubota A Chayama K A multivariate analysis of risk factors for hepatocellular carcinogenesis: A prospective observation of 795 patients with viral and alcoholic cirrhosis. Hepatology 1993; 18: 47–53.

Sugimura T Tsuji Y Sakamoto M Kotoh K Fukutomi T Sakai H Long-term prognosis and prognostic factors of liver cirrhosis in the 1980s. J Gastroenterol Hepatol 1994; 9: 154–61.

Pujol A Pujol J Graus F Rimola A Peri J Mercader JM Hyperintense globus pallidus on T1-weighted MRI in cirrhotic patients is associated with severity of liver failure. Neurology 1993; 43: 65–9.

Stremmel W Wojdat R Groteguth R Zoedler M Ebener T Niederau C Liver function tests in a clinical comparison. Z Gastroenterol 1992; 30: 784–90.

Ott P Keiding S Bass L . Plasma elimination of indocyanine green in the intact pig after bolus injection and during constant infusion: Comparison of spectrophotometry and high pressure liquid chromatography. Hepatology 1993; 18: 1504–15.

Oellerich M Raude E Burdelski M Schultz M Schmidt FW Ringe B Monoethylglycinexylidide formation kinetics: A novel approach to the assessment of liver function. J Clin Chem Clin Biochem 1987; 25: 845–53.

Cakaloglu Y Tredger JM Devlin J Williams R . Importance of cytochrome P450IIIA activity in determining dosage and blood levels of FK506 and cyclosporin in liver transplant recipients. Hepatology 1994; 20: 309–16.

Tanaka K Oda Y Asada A Fujimori M Funae Y . Metabolism of lidocaine by rat pulmonary cytochrome P450. Biochem Pharmacol 1994; 47: 1061–6.

Sallie R Tredger JM Williams R . Extrahepatic production of the lignocaine metabolite monoethylglycinexylidide (MEGX). Biopharm Drug Dispos 1992; 13: 555–8.

Oellerich M Schultz E Polzien F Ringe B Armstrong VW Hartman H Influence of gender on the monoethylglycinexylidide test in normal subjects and liver donors. Ther Drug Monit 1994; 16: 225–31.

Oellerich M Burdelski M Lautz H-U Binder L Pichlmayr R . Predictors of one year pretransplant survival in patients with cirrhosis. Hepatology 1991; 14: 1029–34.

Burdelski M Oellerich M Duwel J Raith H Scheruhn M Ringe B Pre- and post-transplant assessment of liver function in paediatric liver transplantation. Eur J Pediatr 1992; 151 (suppl 1): S39–43.

Oellerich M Burdelski M Ringe B Wittekind C Lamesch P Lautz HU Functional state of the donor liver and early outcome of transplantation. Transplant Proc 1991; 23: 1575–8.

Reding R Wallemacq P de Ville de Goyet J Lerut J Hausleithner V Feyaerts A The unreliability of the lidocaine/monoethylglycinexylidide test for assessment of liver donors. Transplantation 1993; 56: 323–6.

Adam R Azoulay D Astarcioglu I Chiche L Bao YM Bonhomme L Limits of the MEGX test in the selection of liver grafts for transplantation. Transplant Proc 1993; 25: 1653–4.

Meyer-Wyss B Renner E Luo H Scholer A . Assessment of lidocaine metabolite formation in comparison with other quantitative liver function tests. J Hepatol 1993; 19: 133–9.

Arrigoni A Gindro T Aimo G Cappello N Meloni A Benedetti P Monoethylglycinexylidide test: A prognostic indicator of survival in cirrhosis. Hepatology 1994; 20: 383–7.

Ozawa K Aoyama H Yasuda K Shimahara Y Nakatani T Tanaka J Metabolic abnormalities associated with postoperative organ failure: A redox theory. Arch Surg 1983; 118: 1245–51.

Yamamoto Y Ozawa K Okamoto R Kiuchi T Maki A Lin H Prognostic implications of postoperative suppression of arterial ketone body ratio: Time factor involved in the suppression of hepatic mitochondrial oxidation-reduction state. Surgery 1990; 170: 289–94.

Scaiola A MacMathuna P Langley PG Gove CD Hughes RD Williams R . Determination of the ketone body ratio in fulminant hepatic failure. Hepatogastroenterologia 1990; 37: 413–6.

Ozawa K . Living Related Donor Liver Transplantation: Assessment of Graft Viability Based On the Redox Theory. Basel: Karger, 1994.

Matsushita K Kawasaki S Makuuchi M . Arterial ketone body ratio in liver surgery. Hepatology 1994; 20: 331–5.

Fromm H Albert MB . Serum bile acid assays for liver disease: When and how to use them. Gastroenterology 1987; 92: 829–30.

Renner E Wietholtz H Huguenin P Arnaud MJ Preisig R . Caffeine: A model compound for measuring liver function. Hepatology 1984; 4: 38–46.

Cheng WS Murphy TL Smith MT Cooksley WG Halliday JW Powell LW . Dose-dependent pharmacokinetics of caffeine in humans: Relevance as a test of quantitative liver function. Clin Pharmacol Ther 1990; 47: 516–24.

Nagel RA Hayllar KM Tredger JM Williams R . Caffeine clearance and galactose elimination capacity as prognostic indicators in fulminant hepatic failure. Eur J Gastroenterol Hepatol 1991; 3: 907–13.

McFarlane BM . Hepatocellular membrane antigens. In: McFarlane IG Williams R , eds. The Molecular Basis of Autoimmune Hepatitis. Austin, Texas: RG Landes, 1996: 75–104.

Kudo M Todo A Ikekubo K Yamamoto K Vera DR Stadalnik RC . Quantitative assessment of hepatocellular function through in vivo radio-receptor imaging with technetium 99m galactosyl human serum albumin. Hepatology 1993; 17: 814–9.

Davis M . Drugs and abnormal liver function tests. Adv Drug React Bull 1989; 139: 520–3.

Beckett GJ Hayes JD . Glutathione S-transferases: biomedical applications. Adv Clin Chem 1993; 30: 281–380.

Biotrin International. An Evaluation of Hepkit, an EIA for Glutathione-S-transferase, As an Aid in the Diagnosis of Rejection in Liver Transplant Recipients. Dublin: Biotrin International.

Murray JM Rowlands BJ Trinick TR . Indocyanine green clearance and hepatic function during and after prolonged anaesthesia: Comparison of halothane with isoflurane. Br J Anaesth 1992; 68: 168–71.

Beckett GJ Foster GR Hussey AJ Oliveira DBG Donovan JW Prescott LF Plasma glutathione S-transferase and F protein are more sensitive than alanine aminotransferase as markers of paracetamol (acetaminophen)-induced liver damage. Clin Chem 1989; 35: 2186–9.

Hida T Kuwabara M Ariyoshi Y Takahashi T Sugiyura T Hosoda K Serum gluathione S-transferase-pi level as a tumour marker for non-small cell lung cancer. Potential predictive value in chemotherapeutic response. Cancer 1994; 73: 1377–82.

Hirata S Odajima T Kohama G Ishigaki S Niitsu Y . Significance of glutathione S-transferase-pi as a tumor marker in patients with oral cancer. Cancer 1992; 70: 2381–7.

Muraca M Fevery J Blanckaert N . Analytic aspects and clinical interpretations of serum bilirubins. Semin Liver Dis 1988; 8: 137–47.

Lauff JJ Kasper HE Ambrose RT . Separation of bilirubin species in serum and bile by high performance liquid chromatography. J Chromatogr 1981; 226: 629–39.

Weiss JS Gauham A Lauff JJ Sundberg MW Jatlow P Boyer JL The clinical importance of a protein-bound fraction of serum bilirubin in patients with hyperbilirubinaemia. N Engl J Med 1983; 309: 147–50.

Soltes L Sebile B Fugedi A . High performance liquid chromatographic determination of bilirubin on serum proteins. Clin Chim Acta 1991; 201: 35–44.

Singh J Bowers LD . Quantitative fractionation of serum bilirubin species by reversed phase high performance liquid chromatography. J Chrom Biomed Appl 1986; 380: 321–30.

Sundberg MW Lauff JJ Weiss JS Dappen GM Wu T-W Spayd RW Estimation of unconjugated, conjugated and “δ” bilirubin fractions in serum by use of two coated thin films. Clin Chem 1984; 30: 1314–7.

Brett EM Hicks JM Powers DM Rand RN . Delta bilirubin in serum of pediatric patients: Correlations with age and disease. Clin Chem 1984; 30: 1561–4.

Koprowski H Steplewski Z Mitcell K Herlyn M Herlyn D Fuhrer P . Colorectal carcinoma antigens detected by hybridoma antibodies. Somatic Cell Genet 1979; 5: 957–72.

Albert MB Steinberg WM Henry JP . Elevated serum levels of tumour marker CA19-9 in acute cholangitis. Dig Dis Sci 1988; 33: 1223–5.

Haglund C Roberts PJ Jalanko H Kuusela P . Tumour markers CA19-9 and CA50 in digestive tract malignancies. Scand J Gastroenterol 1992; 27: 169–74.

Collazos J Genolla J Ruibal A . CA19-9 in non-neoplastic liver diseases. A clinical and laboratory study. Clin Chim Acta 1992; 210: 145–51.

Ramage JK Donaghy A Farrant JM Iorns R Williams R . Serum tumour markers for the diagnosis of cholangiocarcinoma in primary sclerosing cholangitis. Gastroenterology 1995; 108: 865–9.

Maddrey WC Boitnott JK Bedine MS Weber FL Mezey E White Rl Corticosteroid therapy of alcoholic hepatitis. Gastroenterology 1978; 75: 193–9.

Dickson ER Grambsch PM Fleming TR Fisher LD Langworthy A . Prognosis in primary biliary cirrhosis: Model for decision making. Hepatology 1989; 10: 1–7.

O'Grady JG Alexander GJM Hayllar KM Williams R . Early indicators of prognosis in fulminant hepatic failure. Gastroenterology 1989; 97: 437–45.

Harrison PM O'Grady JG Keays RT Alexander GJM Williams R . Serial prothrombin time as prognostic indicator in paracetamol induced fulminant hepatic failure. BMJ 1990; 301: 964–6.

Moia M Martinelli I Gridelli B Langer M Galmarini D Manucci PM . Prognostic value of haemostatic parameters after liver transplantation. J Hepatol 1992; 15: 125–8.

Bernuau J Samuel D Durand F Saliba F Bourliere M Adam R Criteria for emergency liver transplantation in patients with acute viral hepatitis and factor V below 50% of normal: A prospective study. Hepatology 1991; 14: 49A.

Pereira LMMB Langley PG Bird GLA Hayllar KM Tredger JM Williams R . Coagulation factors V and VIII in relation to severity and outcome in acute alcoholic hepatitis. Alcohol Alcohol 1992; 27: 55–61.

Bilik R Superina RA Poon AO . Coagulation plasma factor levels are early indicators of graft non-function following liver transplantation in children. J Pediatr Surg 1992; 27: 302–6.

Patrassi GM Sartori MT Viero M Boeri G Simioni P Bassi N Protein C, factor VII and prothrombin time as early markers of liver function recovery or failure after liver transplantation. Blood Coagul Fibrionolysis 1993; 4: 863–7.

Violi F Ferro D Basili D Cimminiello C Saliola M Vezza E Prognostic value of clotting and fibrinolytic systems in a follow-up of 165 liver cirrhotic patients. Hepatology 1995; 22: 96–100.

Schuppan D . Structure of the extracellular matrix in normal and fibrotic liver: Collagens and glycoproteins. Semin Liver Dis 1990; 10: 1–10.

Galambos MR Collins DC Galambos JT . A radioimmunoassay procedure for type III procollagen: Its use in the detection of hepatic fibrosis. Hepatology 1985; 5: 38–42.

Gabrielli GB Corrocher R . Hepatic fibrosis and its serum markers: A review. Dig Dis 1991; 9: 303–16.

Savolainen E-R Goldberg B Leo MA Velez M Lieber CS . Diagnostic value of serum procollagen peptide measurements in alcoholic liver disease. Alcohol Clin Expl Res 1984; 8: 384–9.

Niemela O Risteli L Sotaniemi EA Risteli J . Aminoterminal propeptide of type III procollagen in serum in alcoholic liver disease. Gastroenterology 1983; 85: 254–9.

Torres-Salinas M Pares A Caballeria J Jimenez W Heredia D Bruguera M Serum procollagen type III peptide as a marker of hepatic fibrogenesis in alcoholic hepatitis. Gastroenterology 1986; 90: 1241–6.

Nouchi T Worner TM Sato S Lieber CS . Serum procollagen type III N-terminal peptides and laminin P-1 peptide in alcoholic liver disease. Alcohol Clin Expl Res 1987; 11: 287–91.

Lotterer E Gressner AM Kropf J Grobe E von Knebel D Bircher J . Higher levels of serum aminoterminal type III procollagen peptide and laminin in alcoholic than in non-alcoholic cirrhosis of equal severity. J Hepatol 1992; 14: 71–7.

Tanaka Y Minato Y Hasumura Y Takeuchi J . Evaluation of hepatic fibrosis by serum proline and aminoterminal type III procollagen peptide levels in alcoholic patients. Dig Dis Sci 1986; 31: 712–7.

Colombo M Annoni G Donato MF Conte D Martines D Zaramella MG Serum type III procollagen peptide in alcoholic liver disease and idiopathic haemochromatosis: Its relationship to hepatic fibrosis, activity of the disease and iron overload. Hepatology 1985; 5: 475–9.

Bentsen KD Henriksen JH Bendtsen F Horslev-Petersen K Lorenzen I . Splanchnic and renal extraction of circulating type III procollagen aminoterminal propeptide in patients with normal liver function and in patients with alcoholic cirrhosis. Hepatology 1990; 11: 957–63.

Trinchet J-C Hartmann DJ Pateron D Munz-Gotheil C Callard P Ville G Serum type I collagen and N-terminal peptide of type III procollagen in patients with alcoholic liver disease. Relationship to liver histology. Alcohol Clin Expl Res 1992; 16: 342–6.

Wiegand K Zaugg PY Frei A Zimmermann A . Long-term follow-up of serum N-terminal propeptide of collagen type III levels in patients with chronic liver disease. Hepatology 1984; 4: 835–8.

Annoni G Colombo M Canteluppi MC Khlat B Lampertico P Rojkind M . Serum type III procollagen peptide and laminin (Lam-PI) detect alcoholic hepatitis in chronic alcohol abusers. Hepatology 1989; 9: 693–7.

Gabrielli GB Faccioli G Casaril M Capra F Bonazzi L Falezza G Procollagen III peptide and fibronectin in alcohol-related chronic liver disease: Correlations with morphological features and biochemical tests. Clin Chim Acta 1989; 79: 315–22.

van Zanten RAA van Leeuwen RE Wilson JHP . Serum procollagen III N-terminal peptide and laminin P1 fragment concentrations in alcoholic liver disease and primary liver cirrhosis. Clin Chim Acta 1988; 177: 141–6.

Bolarin DM Savolainen E-R Kivirikko KI . Enzymes of collagen synthesis and type III procollagen amino-pro-peptide in serum from Nigerians with hepatocellular carcinoma and other malignant diseases. Int J Cancer 1982; 29: 401–5.

Savolainen E-R Miettinen TA Pikkarainen P Salaspuro MP Kivirikko KI . Enzymes of collagen synthesis and type III procollagen aminopeptide in the evaluation of D-penicillamine and medroxy-progesterone treatments of primary biliary cirrhosis. Gut 1983; 24: 136–42.

Babbs O Smith A Hunt LP Rowan BP Haboubi NY Warnes TW . PIIINP: A marker of disease activity and prognosis in primary biliary cirrhosis. Lancet 1988; i: 1021–4.

Beukers R van Zanten RAA Schalm SW . Serial determination of type III procollagen aminopropeptide serum levels in patients with histologically progressive and non-progressive primary biliary cirrhosis. J Hepatol 1992; 14: 22–9.

Nyberg A Lindqvist U Engstrom-Laurent A . Serum hyaluron and aminoterminal propeptide of type III procollagen in primary biliary cirrhosis: Relation to clinical symptoms, liver histopathology and outcome. J Int Med 1992; 231: 485–91.

McCullough AJ Stassen WN Wiesner RH Czaja AJ . Serum type III procollagen peptide concentration in severe chronic active hepatitis: Relationship to cirrhosis and disease activity. Hepatology 1987; 7: 49–54.

Trinchet JC Hartmann DJ Pateron D Laarif M Gallard P Ville G Serum type I collagen and N-terminal peptide of type III procollagen in chronic hepatitis. Relationship to liver histology and conventional liver tests. J Hepatol 1991; 12: 139–44.

Misaki M Shima T Yano Y Sumita Y Kano U Murata T Basement membrane related and type III procollagen related antigens in serum of patients with chronic viral liver disease. Clin Chem 1990; 36: 522–4.

Murawaki Y Ikuta Y Koda M Kawasaki H . Serum type III procollagen peptide, type IV collagen 7S domain, central triple-helix of type IV collagen and tissue inhibitor of metalloproteinases in patients with chronic viral liver disease. Relationship to liver histology. Hepatology 1994; 20: 780–7.

Muller A Krombholz B Pott G Machnik G Vollandt R Reinhardt M Collagen peptidase and type III procollagen peptide serum levels in chronic liver diseases. Clin Chim Acta 1991; 197: 59–66.

Collazos J Diaz F . Role of the measurement of serum procollagen type III N-terminal peptide in the evaluation of liver diseases. Clin Chim Acta 1994; 227: 37–43.

Raedsch R Stiehl A Sieg A Walker S Kommerell B . Biliary excretion of procollagen type III peptide in healthy humans and in patients with alcoholic cirrhosis of the liver. Gastroenterology 1983; 85: 1265–70.

Sakakihara K Ooshima A Igarashi S Sakakihara J . Immunolocalization of type III collagen and procollagen in cirrhotic liver using monoclonal antibodies. Virchows Arch (A) 1986; 409: 37–46.

Pihlajanremi T Myllyla R Kivirikko KI . Prolyl 4-hydroxylase and its role in collagen synthesis. J Hepatol 1991; 13 (suppl 3): S2–S7.

Mann SW Fuller GC Rodil JV Vidins EI . Hepatic prolyl hydroxylase and collagen synthesis in patients with alcoholic liver disease. Gut 1979; 20: 825–32.

Mezey E Potter JJ Iber FL Maddrey WC . Hepatic collagen proline hydroxylase activity in alcoholic hepatitis: Effect of D-penicillamine. J Lab Clin Med 1979; 93: 92–100.

Mephie JL . Hepatic prolyl hydroxylase activity in human liver disease. Hepatogastroenterology 1980; 27: 277–82.

Nagai Y Yoshiba M . Studies on serum immunoreactive prolyl 4-hydroxylase in liver diseases—its elevation both in hepatocellular damage and cholestatic diseases. Clin Chim Acta 1988; 175: 27–36.

Testa R Bindi P Risso D Borzone S Caglieris S Bardellini E Serum prolyl hydroxylase as an index of fibrogenic activity in chronic active hepatitis and cirrhosis. Eur J Gastroenterol Hepatol 1993; 5: 103–7.

Kropf J Gressner AM Negwer A . Efficacy of serum laminin measurement for diagnosis of fibrotic liver diseases. Clin Chem 1988; 34: 2026–30.

Gressner AM Tittor N . Serum laminin in fibrotic and cirrhotic liver diseases. Protides Biol Fluids Proc Colloq 1986; 34: 597–600.

Collazos J . Serum concentrations of laminin in cirrhosis. Gut 1993; 34: 974–6.

Gressner AM . The predictive value of serum laminin for portal hypertension in chronic liver diseases. Hepatogastroenterology 1988; 35: 95–100.

Niemela O Risteli L Sotaniemi EA Risteli J . Type IV collagen and laminin related antigens in human serum in alcoholic liver disease. Eur J Clin Invest 1985; 15: 132–7.

Naveau S Poynard T Abella A Pignon J-P Poitrine A Agostini H Prognostic value of serum fibronectin concentration in alcoholic cirrhotic patients. Hepatology 1985; 5: 819–23.

Rajabi M Dean DD Woessner JF . High levels of serum collagenase in premature labour. A potential biochemical marker. Obstet Gynecol 1987; 69: 179–86.

Murawaki Y Koda M Yamada S Kawasaki H Shima H Burkhardt H . Serum collagenase activity in patients with chronic liver disease. J Hepatol 1993; 18: 328–34.

Poynard T Aubert A Bedossa P Alella A Naveau S Parof F A simple biological index for detection of alcoholic liver disease in drinkers. Gastroenterology 1991; 100: 1397–402.

Teare JP Sherman D Greenfield SM Simpson J Bray G Catterall AP Comparison of serum procollagen III peptide concentrations and PGA index for assessment of hepatic fibrosis. Lancet 1993; 342: 895–8.

Tygstrup N Winkler K . Galactose blood clearance as a measure of hepatic blood flow. Clin Sci 1958; 17: 1–9.

Molino G Cavanna A Avagnina P Ballarè M Torchio M . Hepatic clearance of D-Sorbitol. Non-invasive test for evaluating functional liver plasma flow. Dig Dis Sci 1987; 32: 753–8.

Keiding S . Editorial. Can intrahepatic shunting be measured? Dig Dis Sci 1991; 9: 1185–8.

Pulsion Medizintechnik. The Volumetric Pilot for Physiological Therapy: COLD Z-021. Munich: Pulsion Medizintechnik.

Potter JM Hichman PE Lynch SV Henderson A Wright M Balderson G Use of monoethylglycinexylidide as a liver function test in the liver transplant recipient. Transplantation 1993; 56: 1385–8.

Jalan R Plevris JN Jalan AR Finlayson ND Hayes PC . A pilot study of indocyanine green clearance as an early predictor of graft function. Transplantation 1994; 58: 196–200.

Doyle HR Marino IR Jabbour N Zetti G McMichael S Early death or retransplantation in adults after orthotopic liver transplantation. Can outcome be predicted? Transplantation 1994; 57: 1028–36.

Devlin J Palmer RM Gonde CE O'Grady J Heaton N Tan KC Nitric oxide generation. A predictive parameter of acute allograft rejection. Transplantation 1994; 58: 592–5.

Adams DH Wang L Hubscher SG Neuberger JM . Hepatic endothelial cells. Targets in liver allograft rejection. Transplantation 1989; 47: 479–82.

Gozzo ML Avolio A Forni F Agnes S Colacicco L Barbaresi G Enzymatic determinations in acute rejection after liver transplantation: Preliminary report on necrosis index. Clin Chim Acta 1993; 214: 175–84.

Adolf J Martin WG Muller DF Beckurts KT Schneider-Eicke J Wittekind C The effect of acute cellular rejection on liver function following orthotopic liver transplantation. Quantitative functional studies with the ¹⁴C aminopyrine breath test. D Med Wochenschr 1992; 117: 1823–8.

Tilg H Nordberg J Vogel W Luger TA Herold M Aulitzky WE Circulating serum levels of interleukin 6 and C-reactive protein after liver transplantation. Transplantation 1992; 54: 142–6.

Lalli E Meliconi R Conte R Mancini A Uguccioni M Stefanini GF Serum markers of immune activation and liver allograft rejection. Dig Dis Sci 1992; 37: 1116–20.

Kita Y Iwaki Y Demetris AJ Starzl TE . Evaluation of sequential serum interleukin 6 levels in liver allograft recipients. Transplantation 1994; 57: 1037–41.

Hamilton G Prettenhofer M Zommer A Hofbauer S Gotzinger P Gnant FX Intraoperative course and prognostic significance of endotoxin, tumor necrosis factor alpha and interleukin 6 in liver transplant recipients. Immunobiology 1991; 182: 425–39.

Holt DW Johnston A Roberts NB Tredger JM Trull AK . Association of Clinical Biochemists Cyclosporin Task Force report. Ann Clin Biochem 1994; 31: 420–46.

Tredger JM Wong PYN . Immunosuppressive drug monitoring and drug interactions. In: Williams R Portmann B Tan KC , eds. The Practice of Liver Transplantation. Edinburgh: Churchill-Livingstone, 1995: 211–34.

Koneru B Leevy CB Klein KM Zweil KM Wilson DJ . Indocyanine green clearance in the evaluation of liver donors. Transpl Proc 1993; 25: 1919–20.

Devlin J Dunne JB Sherwood RA Chambers SM Heaton N Tan KC Relationship between early liver graft variability and enzyme levels in effluent preservation solution. Transplantation 1995; 60: 627–30.

Bronsther OL Rao PN Pinna A Snyder J Cowan S Kramer D Effluent levels of hyaluronic acid can predict ultimate graft outcome after clinical liver transplantation: A prospective series. Transpl Proc 1993; 25: 1538–40.

Nusinovici V Crubille C Opolon P Touboul J-P Darnis F Caroli J . Fulminant hepatitis: An experience based on 137 cases. Gastroenterol Clin Biol 1977; 1: 875–86.

Ramsøe K Andreason PB Ranek L . Functioning liver mass in uncomplicated and fulminant acute hepatitis. Scand J Gastroenterol 1980; 15: 65–72.

Saunders J Wright N Lewis KO . Predicting outcome of paracetamol poisoning by using ¹⁴C-aminopyrine breath test. BMJ 1980; i: 279–80.

Lake JR Sussman NL . Determining prognosis in patients with fulminant hepatic failure: When you absolutely, positively have to know the answer. Hepatology 1995; 21: 879–82.

Pauwels A Mostefa KN Florent C Levy VG . Emergency liver transplantation for acute liver failure. Evaluation of London and Clichy criteria. J Hepatol 1993; 17: 124–7.

Pereira LMMB Langley PG Hayllar KM Tredger JM Williams R . Coagulation factor V and VIII/V ratio as predictors of outcome in paracetamol-induced fulminant hepatic failure: Relation to other prognostic indicators. Gut 1992; 33: 98–102.

Shiffman ML Fisher RA Sanyal AJ Edinboro LE Luketic VA Purdum PP Hepatic lidocaine metabolism and complications of cirrhosis. Implications for assessing patient priority for hepatic transplantation. Transplantation 1993; 55: 830–5.

Pugh RNH Murray-Lyon IM Dawson JL Pietroni MC Williams R . Transections of the oesophagus for bleeding varices. Br J Surg 1973; 60: 646–9.

Albers I Hartmann H Bircher J Creutzfeldt W . Superiority of the Child—Pugh classification to quantitative liver function tests for assessing prognosis of liver cirrhosis. Scand J Gastroenterol 1989; 24: 269–76.

Beuers U Jager F Wahllander A Ansari H Kirsch CM . Prognostic value of the intravenous ¹⁴C-aminopyrine breath test compared to the Child Pugh score and serum bile acids in 84 cirrhotic patients. Digestion 1991; 50: 212–8.

Christensen E Neuberger J Crowe J Altman DG Popper H Portmann B Beneficial effect of azathioprine and prediction of prognosis in primary biliary cirrhosis. Final results of an international trial. Gastroenterology 1985; 89: 1084–91.

Wiesner RH Grambsch PB Dickson ER Ludwig J MacCarty RL Hunter EB . Primary sclerosing cholangitis: Natural history, prognostic factors and survival analysis. Hepatology 1989; 10: 430–6.

Farrant JM Hayllar KM Wilkinson ML Karani J Portmann B Westaby D Natural history and prognostic variables in primary sclerosing cholangitis. Gastroenterology 1991; 100: 1710–7.

Sherman DIN Williams R . Liver damage: Mechanisms and management. Br Med Bull 1994; 50: 1124–38.

Scheuer PJ Lefkowitch JH In: Major problems in pathology. Liver Biopsy Interpretation, 5th edn, Vol. 31. London: WB Saunders, 1994.

MacSween RNM Anthony PP Scheuer PJ Burt AD Portmann BC , eds. Pathology of the Liver. Edinburgh: Churchill Livingstone, 1994.

Johnson PJ McFarlane IG . The Laboratory Investigation of Liver Disease. London: Baillière Tindall, 1989.

Burditt LJ Johnson M Johnson PJ Williams R . Detection of hepatocellular carcinoma-specific alpha-fetoprotein by isoelectric focusing. Cancer 1994; 74: 25–9.

Suzuki H Iino S Endo Y Torii M Miki K . Tumor-specific alkaline phosphatase in hepatoma. Ann NY Acad Sci 1975; 259: 307–20.

Sawubo N Nakagen M Ozaki K Wakabayashi T Toya D Hattori N Clinical evaluation of specific γGTP isoenzyme in patients with hepatocellular carcinoma. Cancer 1983; 51: 327–31.

Liebman HA Furie BC Tong MJ Blanchard RA Lo KJ Lee SD Des-γ-carboxy (abnormal) prothrombin as a serum marker of primary hepatocellular carcinoma. N Engl J Med 1984; 310: 1427–31.

Deacon A . The measurement of 5-hydroxyindoleacetic acid in urine. Ann Clin Biochem 1994; 31: 215–32.

Duffy MJ . New cancer markers. Ann Clin Biochem 1989; 25: 379–87.

Beastall GH Cook B Rustin GJS . A review of the role of the established tumour markers. Ann Clin Biochem 1991; 28: 5–18.

Fremont S Champigneulle B Gerard P Felden F Lambert D Gueant JL Blood transcobalamin levels in malignant hepatoma. Tumor Biol 1991; 12: 353–9.

Ishak KG Sharp HL . Metabolic errors and liver disease. In: MacSween RNM Anthony PP Scheuer PJ Burt AD Portmann BC , eds. Pathology of the Liver. Edinburgh: Churchill Livingstone, 1994: 123–218.

Kalsheker NA . Molecular pathology of α₁-antitrypsin deficiency and its significance to clinical medicine. Q J Med 1994; 87: 653–8.

Lomas DA Evans DLI Finch J Carrell RW . The mechanism of Z alpha-1-antitrypsin accumulation in the liver. Nature 1992; 357: 605–7.

Psacharopoulous HT Mowat AP Cook PJL Carlisle PA Portmann B Rodeck CH . Outcome of liver disease associated with α₁-antitrypsin deficiency (PiZ). Arch Dis Child 1983; 58: 882–7.

Allen RC Harley RA Talamo RC . A new method for determination of alpha-1-antitrypsin phenotypes using isoelectric focusing on polyacrylamide gel slabs. Am J Chem Pathol 1974; 62: 732–9.

Lai EC Kao FT Law ML Woo SLC . Assignment of the alpha-1-antitrypsin gene and a sequence related gene to human chromosome 14 by molecular hybridization. Am J Hum Genet 1983; 35: 385–92.

Gregersen N Winter V Petersen KB Koch J Kolvraa S Rudiger N Detection of point mutations in amplified single copy genes by biotin-labelled oligonucleotides: Diagnosis of variants of alpha-1-antitrypsin. Clin Chim Acta 1989; 182: 151–64.

Chambers SM Norman MR . Genotyping of alpha-1-antitrypsin genes by polymerase chain reaction. Proc Natl Meeting Assoc Clin Biochem 1992: 43.

Abbott CM McMahon CJ Whitehouse DB Povey S . Prenatal diagnosis of α₁antitrypsin using polymerase chain reaction. Lancet 1988; i: 763–4.

Hejtmancik JF Ward PA Mansfield T Sifers RN Harris S Cox DW . Prenatal diagnosis of α₁-antitrypsin deficiency by RFLPs and comparison with oligonucleotide probe analysis. Lancet 1986; ii: 767–9.

Chambers SM Norman MR . Genotyping of alpha-1-antitrypsin genes by polymerase chain reaction. Proc Natl Meeting Assoc Clin Biochem 1994: 50.

Newton CR Graham A Heptinstall LE Powell SJ Summers C Kalsheker N Analysis of point mutation in DNA. The amplification refractory mutation system (ARMS). Nuc Acid Res 1989; 17: 2503–16.

Searle J Kerr JFR Halliday JW Powell LW . Iron storage disease. In: MacSween RNM Anthony PP Scheuer PJ Burt AD Portmann BC , eds. Pathology of the Liver. Edinburgh: Churchill Livingstone, 1994.

Frydman M Bonné-Tamir B Farrer LA Conneally PM Magazanik A Ashbel A Assignment of the gene for Wilsons disease to chromosome 13. Proc Natl Acad Sci USA 1985; 82: 1819–21.

Petrukhin K Fischer SG Pirastu M Tanzi RE Chernov I Devoto M Mapping, cloning and genetic characterisation of the region containing the Wilson disease gene. Nature Genet 1993; 5: 338–43.

Tanzi RE Petrukhin K Chernov I Pellequer JL Wasco W Ross B The Wilson disease gene is a copper transporting ATPase with homology to the Menkes disease gene. Nature Genet 1993; 5: 344–50.

Bull PC Thomas GR Rommens JM Forbes JR Cox DW . The Wilson disease gene is a putative copper transporting P-type ATPase similar to the Menkes gene. Nature Genet 1993; 5: 327–37.

Da Costa CM Baldwin D Portmann B Lolin Y Mowat AP Mieli-Vergani G . Value of urinary copper excretion after penicillamine challenge in the diagnosis of Wilson's disease. Hepatology 1992; 15: 609–15.

Salaspuro M . Characteristics of laboratory markers of alcohol related organ damage. Scand J Gastroenterol 1989; 24: 769–80.

Panteghini M Falsetti F Chiari E Malchiodi A . Determination of aspartate aminotransferase isoenzymes in hepatic diseases—preliminary findings. Clin Chim Acta 1983; 128: 133–40.

Kamicke W Fujikawa M Koseki M Sumimura J Miyata M Kawashima Y Different patterns of leakage of cytosolic and mitochondrial enzymes. Clin Chim Acta 1989; 185: 265–70.

Nalpas B Poupon RE Vassault A Hauzanneau P Sage Y Schellenberg F Evaluation of mAST/tAST ratio as a marker of alcohol misuse in a non-selected population. Alcohol Alcohol 1989; 24: 415–9.

Nalpas P Vassault A Charpin S Lacour B Berthelot P . Serum mitochondrial aspartate aminotransferase as a marker of chronic alcoholism: Diagnostic value and interpretation in a liver unit. Hepatology 1986; 4: 608–14.

Kamimoto Y Horiuchi S Tanase S Morino Y . Plasma clearance of intravenously injected aspartate aminotransferase isozymes: Evidence for preferential uptake by sinusoidal liver cells. Hepatology 1985; 5: 367–75.

Benamouzig R Boutron A Goenner S Ink O Buffet C . Serum mitochondrial aspartate aminotransferase in extra-hepatic cholestasis: Evolution after bile duct endoscopic clearance. Eur J Gastroenterol Hepatol 1991; 3: 551–4.

Lauterburg BH Liang D Schwarzenbach FA Breen KJ . Mitochondrial dysfunction in alcoholic patients as assessed by breath analysis. Hepatology 1993; 17: 418–22.

Stibler H Kjellin KG . Isoelectric focusing and electrophoresis of the CSF proteins in tremor of different origins. J Neurol Sci 1976; 30: 269–85.

Stibler H Borg S . Carbohydrate composition of serum transferrin in alcoholic patients. Alcohol Clin Expl Res 1986; 10: 61–4.

Stibler H Borg S Joustra M . Micro anion exchange chromatography of carbohydrate-deficient transferrin in relation to alcohol consumption. Alcohol Clin Expl Res 1986; 10: 535–44.

Storey EL Mack U Powell LW Halliday JW . Use of chromatofocusing to detect a transferrin variant in serum of alcoholic subjects. Clin Chem 1985; 31: 1543–5.

Stibler H . Carbohydrate-deficient transferrin in serum: A new marker of potentially harmful alcohol consumption reviewed. Clin Chem 1991; 37: 2029–37.

Stibler H Borg S . The value of carbohydrate-deficient transferrin as a marker of high alcohol consumption. In: Kuriyama K Takada A Ishii H , eds. Biomedical and Social Aspects Of Alcohol and Alcoholism, Vol. 5. Amsterdam: Elsevier Science Publishers BV, 1988: 393–8.

Takase S Takada A Tsutsumi M Matsuda Y . Biochemical markers of chronic alcoholism. Alcohol 1985; 2: 405–10.

Yamauchi M Hirakawa J Maezawa Y Nishikawa F Mizuhara Y Ohata M Serum level of carbohydrate-deficient transferrin as a marker of alcoholic liver disease. Alcohol Alcohol 1994; suppl 1B: 3–8.

Behrens U Worner T Lieber CS . Changes in carbohydrate deficient transferrin levels after alcohol withdrawal. Alcohol Clin Expl Res 1988; 12: 539–44.

Stibler H Borg S . Glycoprotein glycosyltransferase activities in serum in alcohol-abusing patients and healthy controls. Scand J Clin Lab Invest 1991; 51: 43–51.

Guasch R Renau-Piqueras J Guerri C . Chronic ethanol consumption induces accumulation of proteins in the liver Golgi apparatus and decreases galactosyltransferase activity. Alcohol Clin Expl Res 1992; 16: 942–8.

Stibler H Hultvrantz R . Carbohydrate deficient transferrin (CDT) in patients with liver diseases. Alcohol Clin Expl Res 1987; 11: 468–73.

Conigrave KM Saunders JB Whitfield JB . Diagnostic tests for alcohol consumption. Alcohol Alcohol 1995; 30: 13–26.

Davis VE Brown H Huff JA Cashaw JL . The alteration of serotonin metabolism to 5-hydroxytryptophol by ethanol ingestions in man. J Lab Clin Med 1967; 69: 132–40.

Voltaire A Beck O Borg S . Urinary 5-hydroxytryptophol: A possible marker of recent alcohol consumption. Alcohol Clin Expl Res 1992; 16: 281–5.

Maezawa Y Yamamuchi M Toda G . Association between restriction fragment length polymorphism of the human cytochrome P450IIE1 gene and susceptibility to alcoholic liver cirrhosis. Am J Gastroenterol 1994; 89: 561–5.

Zuckerman AJ Thomas HC . Viral Hepatitis: Scientific Basis and Clinical Management. Edinburgh: Churchill-Livingstone, 1993.

Saleh MG Tibbs CJ Koskinas J Pereira LMMB Bomford AB Portmann B Hepatic and extrahepatic C virus replication in relation to response to interferon therapy. Hepatology 1994; 20: 1399–404.

De Bisceglie A . Epidemiology and diagnosis of hepatitis D. In: Zuckerman AJ Thomas HC , eds. Viral Hepatitis: Scientific Basis and Clinical Management. Edinburgh: Churchill-Livingstone 1993: 351–61.

O'Donohue J Williams R . Review Article: Primary Biliary Cirrhosis. Q J Med 1996; 89: 5–15.

Basscndine MF Yeaman SJ . Serological markers of primary biliary cirrhosis: Diagnosis, prognosis and subsets [Editorial]. Hepatology 1992; 15: 545–7.

International Working Party Report. Terminology of chronic hepatitis. Am J Gastroenterol 1995; 90: 181–9.

Farrant JM Doherty DG Donaldson PT Vaughan RW Hayllar KM Welsh KI Amino acid substitutions at position 38 of the DRβ polypeptide confer susceptibility to and protection from primary sclerosing cholangitis. Hepatology 1992; 16: 390–5.

Johnson PJ McFarlane IG . Meeting report: International Autoimmune Hepatitis Group. Hepatology 1993; 18: 998–1005.

Pessayre D Larrey D . Acute and chronic drug-induced hepatitis. Baillières Clin Gastroenterol 1988; 2: 385–422.

Zimmerman HJ . Hepatotoxicity: the Adverse Effects of Drugs and Other Chemicals on the Liver. New York: Appleton-Century-Crofts, 1978.

Kenna JG Neuberger J Williams R . Evidence for expression in human liver of halothane-induced neoantigens recognised by antibodies in sera from patients with halothane hepatitis. Hepatology 1988; 8: 1635–41.

Neuberger J Williams R . Immune mechanisms in tienilic acid associated hepatotoxicity. Gut 1989; 30: 515–9.

Neuberger J Kenna JG Nouri-Aria K Williams R . Antibody mediated hepatocyte injury in α-methyl dopa induced hepatotoxicity. Gut 1985; 26: 1233–6.

Leeder JS Riley RJ Cook VA Spielberg SP . Human anti-cytochrome P450 antibodies in aromatic anticonvulsant-induced hypersensitivity reactions. J Pharmacol Expl Ther 1992; 263: 360–7.

Homberg JC Stelly M Andreis I Abuaf N Saadoun F André J . A new anti-mitochondrial antibody (anti-M6) in iproniazid-induced hepatitis. Clin Expl Immunol 1982; 47: 93–103.

Kappus H Remmer H . Irreversible protein binding of ¹⁴C-imipramine with rat and human liver microsomes. Biochem Pharmacol 1975; 24: 1079–85.

Pessayre D Larrey D Funck-Brentano C Benhamou JP . Drug interactions and hepatitis induced by some macrolide antibiotics. J Antimicrob Chemother 1985; 16 (suppl A): 181–94.

Gluud C Bahnsen M Bennett P Dietrichson O Henriksen JH Johnson SG Oral testosterone load related to liver function in man with alcoholic cirrhosis. Scand J Gastroenterol 1983; 18: 391–4.

Kadohara M Kawasaki H Hirayama C . Serum bile acids and oral ursodeoxycholic acid tolerance test in the diagnosis of oesophageal varices. Gastroenterol Jpn 1987; 22: 614–20.

Donaldson BW Gopinath R Wanless IR Phillips MJ Cameron R Roberts EA The role of transjugular biopsy in fulminant hepatic failure: Relation to other prognostic indicators. Hepatology 1993; 18: 1370–4.