The Abbott TDx™ is frequently used for therapeutic drug monitoring of phenytoin concentrations, but reagent costs are high. In an attempt to reduce these costs, we investigated the preparation of in-house reagents. A commercial antiserum was available at reasonable cost and the fluorescent tracer was easily prepared. We describe the preparation of these reagents and their application to the TDx™ system. Substantially reduced costs were obtained using the in-house reagents.
WilsonJFTsanaclisLMPerrettJEWilliamsJWicksJFCRichensAPerformance of techniques for measurement of therapeutic drugs in serum. A comparison based on external quality assessment data. Ther Drug Monit1992; 14: 98–106
3.
SungENeeleyWEA cost-effective system for performing therapeutic drug assays 1. Optimization of the theophylline assay. Clin Chem1985; 31: 1210–15
4.
GoochJCCaldwellRTurnerGJColbertDLCost effective EMIT assays, for drugs of abuse in urine, using the Eppendorf EPOS analyser. J Immunoassay1992; 13: 85–96
5.
ColbertDLMeasurement of Phenytoin in serum using in-house reagents employing the Syva enzyme multiplied immunoassay technique principle. Ther Drug Monit1992; 14: 386–90
6.
McGregorARCrookall-GreeningJOLandonJSmithDSPolarisation fluoroimmunoassay of Phenytoin. Clin Chim Acta1978; 83: 161–6
7.
PourfarzanehMWhiteGWLandonJSmithDSCortisol directly determined in serum by fluoroimmunoassay with magnetizable solid phase. Clin Chem1980; 26: 730–3
8.
EreminSACoxinREColbertDLLandonJSmithDSUrinary continine fluoroimmunoassay for smoking status screening adapted to an automated analyser. Analyst1992; 117: 697–9
9.
FrettholdDPrimidone, phenobarbital, Phenytoin, and carbamazepine in plasma using resin extraction and reversed-phase HPLC. In: SunshineEJatlowPI, eds. Methodology for Analytical Toxicology, Vol II. Florida, USA: CRC Press, 1982: 81–5
