17-oxogenic steroids have been analysed by gas chromatography (GC) with capillary column and flame ionization detector. The best separation of steroid products was gained after ethyloxime-trimethylsilyl ether formation. The value of the GC method as a screening procedure for patients with suspected adrenal disease is illustrated in selected cases where the diagnosis was equivocal from data derived by RIA analysis of plasma steroids.
RuddBT. Urine 17-oxogenic and 17 oxosteroids. Ann Clin Biochem1983; 20, 65–71.
2.
HonourJWPriceDATaylorDFMarsdenHBGrantDB. Steroid Biochemistry of virilising adrenal tumours in childhood. Eur J Paediatr1984; 142, 165–9.
3.
HonourJWDillonMJShackletonCHL. Analysis of steroids in urine for differentiation of pseudohypoaldosteronism and aldosterone biosynthetic defect. J Clin Endocrinol Metab1982; 54, 325–51.
4.
ShackletonCHLTaylorNFHonourJW. An Atlas of Gas Chromatographic Profiles of Neutral Steroids in Health and Disease. Delft: Packard-Becker B. V., 1980.
5.
FewJD. A method for the analysis of urinary 17-hydroxycorticosteroids. J Endocrinol1961; 21, 31–46.
6.
MetcalfeJD. A rapid method for measuring 17-hydroxycorticosteroids in urine. J Endocrinol1963; 26, 415–23.
7.
MakinHLJ. The gas liquid chromatography of steroid formates: An application in congenital adrenal hyperplasia. J Endocrinol1970; 47, 55–64.
8.
TrochaPd'AmatoNA. A method of screening urinary steroids by gas chromatography. Clin Chem1978; 24, 193–9.
9.
CrookD.The routine measurement of urinary 17 oxosteroids and 17 oxogenic steroids using gas liquid chromatography. Clin Chim Acta1972; 40, 43–51.
10.
EveridgeJ.A case of pseudohermaphroditism and adrenalism. Proc Royal Soc Med1945; 38, 649.
11.
RatcliffeWAMacClureJPAuldWHHonourJWFraserRRatcliffeJG. Precocious pseudopuberty due to rare form of congenital adrenal hyperplasia. Ann Clin Biochem1972; 19, 145–50.
12.
HonourJWAndersonJNShackletonCHL. Difficulties in the diagnosis of congenital adrenal hyperplasia in early infancy: The 11β-hydroxylase deficiency. Acta endocrinologica1983; 103, 101–9.
CorrallRJMWakelinKO'HareJPO'BrienIADIsmailAAAHonourJW. 5α reductase deficiency: Diagnosis via abnormal levels of reduced testosterone derivatives. Acta endocrinologica1984; 107, 538–43.
15.
BrooksRV. Fractionation of urinary steroids prior to gas chromatography. Mem Soc Endocr1967; 16, 143–54.
16.
MakinHLJTraffordDJH. Gas-liquid chromatographic fractionation of the urinary 17-oxo and 17-oxogenic steroids; a simple qualitative aid in the diagnosis and treatment of adrenocortical dysfunction. Clin Chim Acta1971; 32, 299–302.
17.
TraffordDJHMakinHLJ. Rapid quantitative gas-chromatographic fractionation of the major urinary 17-oxosteroids and 17-oxogenic steroids. Clin Chim Acta1872; 40, 421–30.
18.
PettittBRKingGS. The electron impact mass spectra of some clinically important androsterols and androsterones as their formate derivatives. Biomed Mass Spectrom1979; 6, 162–4.
19.
TaylorNFCurnowDHShackletonCHL. Analysis of glucocorticoid metabolites in the neo natal period: Catabolism of cortisone acetate by an infant with 21 hydroxylase deficiency. Clin Chim Acta1978; 85, 219–29.
20.
HonourJWShackletonCHL. Differences in metabolism of corticosterone by the newborn and adult human. Biochem Soc Trans1980; 6, 775–8.