A high-pressure liquid chromatography procedure for the measurement of free catecholamines in urine is described. ‘On-line’ sample preparation using a cartesian robot combined with the ASTED™ process ensures minimal operator intervention and reduced costs.
References
1.
StenstromGSjogrenBWaldenstromJ.Excretion of adrenaline, noradrenaline, vanilmandelic acid and metanephrines in 64 patients with phaeochromocytoma. Acta Med Scand1983; 214: 145–52.
DavidsonDFFitzpatrickJ.A simple, optimised and rapid assay for urinary free catecholamines by HPLC with electrochemical detection. Ann Clin Biochem1985; 22: 297–303.
4.
WesterinkBHCBoskerFJOHanlonJF. Use of alumina, Sephadex G10, and ion exchange columns to purify samples for determination of epinephrine, norepinephrine, dopamine, homovanillic acid and 5-hydroxyindoleacetic acid in urine. Clin Chem1982; 28: 1745–8.
5.
PeastonRT. Routine determination of urinary free catecholamines by high performance liquid chromatography with electrochemical detection. J Chromatogr1988; 424: 263–72.
6.
WuAHBGarnetTG. Preparation of urine samples for liquid chromatographic determination of catecholamines: Bonded phase phenylboronic acid, cation exchange resin and alumina adsorbents compared. Clin Chem1985; 31: 298–302.
7.
WeickerHFeraudiMHaegeleHPlutoR.Electrochemical detection of catecholamines in urine and plasma after separation with HPLC. Clin Chim Acta1984; 141: 17–25.
8.
CapmannTLStrasserJM. Rapid extraction of urinary catecholamines and separation by liquid chromatography with electrochemical detection. J Liquid Chromatogr1984; 2: 402–3.
9.
MacdonaldIALakeDM. An improved technique for extracting catecholamines from body fluids. J Neurosci Methods1985; 13: 239–48.
10.
CooperJDHTurnellDC. Automatic preparation of human serum samples for analysis of the drug enoximone and its sulphoxide metabolite using high-performance liquid chromatography. J Chromatogr1986; 380: 109–16.
11.
CooperJDHTurnellDC. Automated sequential process for preparing samples for analysis by high-performance liquid chromatography. J Chromatogr1987; 395: 613–21.
12.
TurnellDCCooperJDHGreenBHughesGWrightDJ. Totally automated assay for cortisol and associated glucocorticoids in serum with use of ASTED sample preparation. Clin Chem1988; 34: 1816–20.
13.
CooperJDHTurnellDCGreenBVarillonF.Automated sequential trace enrichment of dialysates and robotics: A technique for the preparation of biological samples prior to HPLC. J Chromatogr1988; 456: 53–69.
14.
TurnellDCCooperJDH. A concurrent process for the automatic preparation of biological samples combined with high-pressure liquid chromatographic analysis. J Automatic Chem1986; 8: 151–4.
MoyerTPJiangNTyceGMAnalysis for urinary catecholamines by liquid chromatography with amperometric detection: Methodology and clinical interpretation of results. Clin Chem1979; 25: 256–63.
