Abstract
HPLC measurement of the major physiological form of thiamin, thiamin diphosphate, in erythrocytes (ETDP) has been assessed as an indicator of thiamin status and compared with erythrocyte transketolase activity (ETKA) before and after activation with exogenous thiamin diphosphate (TDP effect). The comparison was made by following the response of the parameters to incubation of erythrocytes with thiamin and by measurements in alcoholic, elderly and student groups.
All parameters were responsive to the thiamin content of their environment, but ETDP was at least as sensitive as TDP effect and more sensitive than ETKA in reflecting thiamin uptake and utilisation by erythrocytes. There was a better correlation between ETDP and ETKA than between ETDP and TDP effect. All the groups showed a significant positive correlation between ETDP and ETKA, but only the elderly and the alcoholic groups showed a significant negative correlation between ETDP and TDP effect. The lack of correlation between ETDP and TDP effect in the student group may be due to the relative imprecision of TDP effect measurements at the lower end of the reference range.
ETDP is more stable than ETKA in frozen erythrocytes, is easier to standardise and is not affected by various factors which can influence the enzyme assay. Measurement of ETDP is, therefore, recommended for first line assessment of thiamin status.