This paper describes a highly sensitive small scale enzyme immunoassay for human chorionic gonadotropin (hCG) in pregnancy urine using a micro-transferable plastic bead (MTPB) as solid phase.
Results of the present method for determination of 40 samples of pregnancy urine correlated well with those of the commercial radioimmunoassay and a better precision was achieved. This solid phase method may be widely applied to the immunoassay of small volumes.
References
1.
ImagawaMIshikawaEYoshitakeSA sensitive and specific sandwich enzyme immunoassay for human thyroid-stimulating hormone. Clin Chim Acta1982; 126: 227–36.
2.
ImagawaMHashidaSIshikawaEComparison of β-D-galactosidasc from Escherichia Coli and horseradish peroxidase as labels of anti-human ferritin Fab′ by sandwich enzyme immunoassay technique. J Biochem1984; 96: 659–64.
3.
SuzukiNKonishiEFujitaIIshikawaE. A highly sensitive colorimetric sandwich enzyme immunoassay for human chorionic gonadotropin using specific antibodies against the carboxy-terminal portion of the β-subunit. Clin Chim Acta1985; 150: 247–53.
4.
RuanK-HHashidaSIshikawaE.A micro-scale preparation of affinity-purified Fab′-enzyme conjugates with high purity for enzyme immunoassay. Anal Letts1984; 17 (B18): 2075–90.
5.
VaitukaitisJRobbinsJBNieschlaGERossGT. A method for producing specific antisera with small doses of immunogen. J Clin Endocrinol1971; 33: 988–91.
6.
IshikawaEImagawaMHashidaSEnzyme-labeling of antibodies and their fragments for enzyme immunoassay and immunohistochemical staining. J Immunoassay1983; 4 (3): 209–27.
7.
HashidaSImagawaMInoueSMore useful maleimide compounds for the conjugation of Fab′ to horseradish peroxidase through thiol groups in the hinge. J Appl Biochem1984; 6: 56–63.
8.
ZaitsuKOhkurtaY. New fluorogenic substrates for horseradish peroxidase. Rapid and sensitive assays for hydrogen peroxide and the peroxidase. Anal Biochem1980; 109: 109–13.