Oestradiol in serum was determined with a simple enhanced chemiluminescent enzyme immunoassay. The assay is based on oestradiol labelled with horseradish peroxidase and the IgG fraction of an oestradiol antiserum coated on a black polystyrene microtitre plate. The enzyme activity of bound label was determined using a p-hydroxycinnamic acid-enhanced chemiluminescent reaction.
The assay was sensitive (1·8 fmol/well), precise (intra- and inter-assay CV 4–10% and 8–12%, respectively for sample concentration in the range 122–1330 pmol/L) and showed good agreement with conventional radioimmunoassays (r=0·99).
References
1.
HaningRVLevinRMBehrmanHRPlasma estradiol window and urinary estriol glucuronide determination for monitoring menopropin induction of ovulation. Obstet Gynecol1979; 54: 442–7.
2.
Riad-FahmyDReadGFJoyceBGWalkerRF. Steroid immunoassays in endocrinology. In: VollerABartlettABidwellD, eds. Immunoassays for the 80's. Lancaster: MTP Press, 1980, 205–61.
3.
JoshiUMShahHPSankolliGM. Penicillinase as a marker in enzyme-linked immunosorbent assay for steroid hormones. J Steroid Biochem1983; 19: 419–21.
4.
RodaAGirottiSPiacentiniALDevelopment of a sensitive direct luminescent enzyme immunoassay for plasma estradiol-17β. Anal Biochem1986; 156: 267–73.
5.
AucouturierPPreud'hommeJLLubocinnskyB. Fluorescence polarisation immunoassay of estradiol. Diagn Immun1983; 1: 310–4.
6.
KimJBBarnardGJCollinsWPMeasurement of plasma estradiol-17β by solid-phase chemiluminescence immunoassay. Clin Chem1982; 28: 1120–4.
7.
De BoeverJKohenFVandekerckhoveD. Solid-phase chemiluminescence immunoassay for plasma estradiol-17β during gonadotropin therapy compared with two radioimmunoassays. Clin Chem1983; 29: 2068–72.
8.
De BoeverJKohenFUsanachittCDirect chemiluminesccnce immunoassay for estradiol in serum. Clin Chem1986; 32: 1895–900.
9.
NicolasJCBoussiouxAMBoularanAMBioluminescent assay of femtomole levels of estrone and estradiol. Anal Biochem1983; 135: 141–5.
ThorpeGHGKrickaLJMoseleySBWhiteheadTP. Phenols as enhancers of the chemiluminescent horseradish peroxidase-luminol-hydrogen peroxide reaction: Application in luminescence monitored enzyme immunoassays. Clin Chem1985; 31: 1335–41.
12.
WangHXGeorgeJThorpeGHGEnhanced luminescence enzyme immunoassay for factor VIII related antigen. J Clin Pathol1985; 38: 317–9.
13.
PesceMAStrandeCS. A new micromethod for determination of protein in cerebrospinal fluid and urine. Clin Chem1973; 19: 1265–7.
14.
Van WeemenBKBoschAMGDawsonECSchuursAHWM. Enzyme immunoassay of steroids. Possibilities and pitfalls. J Steroid Biochem1979; 11: 147–51.
15.
SufiSBDonaldsonAJeffcoateSL. Method Manual of WHO Reagent Program. Special Program of Research in Human Reproduction. WHO, Geneva, 1985.
16.
KandeelFRRuddBTMorrisRButtWR. A simple method for counting antibody bound labelled steroids. Steroids1976; 28: 755–62.
17.
KandeelFRG. Development and diagnostic applications of hormone radioimmunoassays in gynaecological endocrinology, PhD Thesis, 1979, University of Birmingham.
18.
MertensRLiedtkeRJBatjerJD. Evaluation of a radioimmunoassay for estradiol in unextracted serum. Clin Chem1983; 29: 1961–3.
19.
MucchielliAAusselCMasseyeffR. Binding of estradiol to horseradish peroxidase and horse heart microperoxidase. Biochem Biophys Res Commun1980; 94: 894–900.
