A spectrophotometric method for the simultaneous calculation of the concentrations of oxyhaemoglobin, methaemoglobin and bilirubin in cerebrospinal fluid is described, based on the first and second derivative spectrum between 350 and 650 nm. Specificity compares favourably with other methods currently in use. The proposed method minimises the effect of turbidity and permits the measurement of concentrations below 0–3 μmol/L.
References
1.
MilianGChirayM.Xantochromie du liquide cephalorachidien. Bull Soc Anat de Paris1902; 4: 550–2.
2.
BarrowsLJHunterFTBankerBQThe nature and clinical significance of pigments in the cerebrospinal fluid. Brain1955; 78: 59–80.
3.
van der MeulenJ. P. Cerebrospinal fluid xanthochromia: an objective index. Neurology (Minneap.)1966; 16: 170–8.
4.
KronholmVLintrupJ. Spectrophotometric investigation of the cerebrospinal fluid in the near-ultraviolet region. Acta Psychial Neurol Scand1960; 35: 314–29.
5.
KronholmV.Speclropholometric investigation of spinal fluid. Wld Neurol, 1960; 2: 435–9.
6.
WalgrcnNGBergströmK. Determination of haem derivatives in the cerebrospinal fluid: a semi-quantitative method. J Neurol Neurosurg Psychial, 1983; 46: 653–8.
7.
VermeulenMvan GijnJBlijenbergB G. Spectrophotometric analysis of CSF after subarachnoid hemorrhage: limitations in the diagnosis of rebleeding. Neurology (NY)1983: 33: 112–4.
8.
KjellinKGSöderströmC E. Diagnostic significance of CSF spectrophotometry in cerebrovascular diseases. J Neurol Sci1974; 23: 359–69.
9.
KjellinKGSteinerL. Spectrophotometry of cerebrospinal fluid in subacute and chronic subdural hacmatomas. J Neurol Neurosurg Psychial1974; 37: 1121–7.
10.
NorrvingBOlssonJ E. The diagnostic value of spcctrophotometric analysis of the cerebrospinal fluid in cerebral hematomas. J Neurol Sci1979; 44: 105–14.
AmazonKSoloniFRywlinA M. Separation of bilirubin from hemoglobin by recording derivative spectrophotometry. Am J Clin Path1981; 75: 519–23.
13.
ParksJWorthHGJCarboxyhemoglobin determination by second derivative spectroscopy. Clin Chem1985; 31: 279–81.
14.
MerrickMFPardueH L. Evaluation of absorption and first- and second-derivative spectra for simultaneous quantification of bilirubin and hemoglobin. Clin Chem1986; 32: 598–602.
15.
WeiserWEPardueH L. Evaluation of multi-wavelength derivative spectra for quantitative applications in clinical chemistry. Clin Chem1983; 29: 1673–7.
16.
SanderinkGJCMvan RijnHJMQuantitative measurement of plasma hemoglobin by second derivative spectrophotometry. Clin Chim Acta1985; 146: 65–73.
17.
van RijnHJMSanderinkGJCMPlasma hemoglobin determination by second derivative spectrophotometry. Isr J Clin Biochem Lab Sci1985; 4: 30.
18.
van KampenEJZijlstraW G.Spectrophotometry of hemoglobin derivatives. Adv Clin Chem1983; 23: 199–257.
19.
WinkelmanJCannonDCJacobsS L. Liver function tests, including bile pigments. In: Henry RJ, Cannon DC, Winkelman JW, eds. Clinical Chemistry, Principles and Technics, 2nd edn.Harper and Row, 1974; 1003–109.
